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anti-Human MCL-1 antibody for Immunoprecipitation

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • bcl2-L-3
  • BCL2L3
  • EAT
  • mcl-1
  • Mcl-1
  • MCL1-ES
  • mcl1/EAT
  • MCL1L
  • MCL1S
  • TM
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
325
106
72
10
9
5
4
3
3
2
1
1
1
Host
Rabbit
221
93
20
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
6
5
5
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
280
144
115
86
83
53
33
27
26
22
7
6
1
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Catalog No. ABIN222889
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000890107 ABIN967637 IP WB Mouse IgG1, kappa C-Term Log in to see 22-Mcl 6
0.000890107 ABIN126984 IHC (p) IP WB Rabbit Log in to see Polyclonal
0.000890107 ABIN967399 IHC (f) IHC (fro) IP WB Rabbit Ig Fraction AA 121-139 Log in to see Polyclonal 6
0.000890107 ABIN180387 IHC (p) IP WB Mouse IgG1 AA 1-327, N-Term Log in to see RC13
0.000890107 ABIN1873661 IHC IP WB Rabbit IgG Log in to see Polyclonal
0.000890107 ABIN605039 IHC (p) IP WB Rabbit Log in to see Polyclonal
0.000890107 ABIN4333160 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 3
0.000890107 ABIN386368 IP WB Biotin Mouse IgG1, kappa AA 1-327 Log in to see RC13
0.000890107 ABIN233420 IP WB Mouse IgG1, kappa AA 1-327 Log in to see
0.000890107 ABIN599894 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 1-327 Log in to see
0.000890107 ABIN386369 IP WB Biotin Mouse IgG1, kappa AA 1-327 Log in to see RC13
0.000890107 ABIN285856 IP IHC WB Rabbit Log in to see Polyclonal
0.000890107 ABIN209035 IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 Log in to see
0.000890107 ABIN2311215 IP IHC ELISA WB Mouse IgG1 kappa AA 1-327 Log in to see 0-N-401
0.000890107 ABIN4333156 ELISA IP WB Mouse IgG AA 206-226 Log in to see RB0961-RB0962
0.000890107 ABIN2737149 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.000890107 ABIN4904325 FACS ICC IF IHC IP WB Rabbit IgG Log in to see
0.000890107 ABIN1912584 IP Rabbit IgG AA 125-175 Log in to see Polyclonal
0.000890107 ABIN1912586 IP WB Rabbit IgG AA 75-125 Log in to see Polyclonal
0.000890107 ABIN2311192 IP IHC ELISA WB Mouse IgG1 AA 1-327 Log in to see 2B2-53

Top referenced anti-MCL-1 antibody for Immunoprecipitation

Similar anti-MCL-1 Antibodies

Application / Reactivity Human Monkey Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 274 Antibodies 5 Antibodies 72 Antibodies 50 Antibodies
Immunoprecipitation (IP) 26 Antibodies 3 Antibodies 9 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 86 Antibodies 1 Antibodies 17 Antibodies 14 Antibodies
Immunohistochemistry (IHC) 116 Antibodies 3 Antibodies 23 Antibodies 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(325), (106), (72), (10), (9), (5), (4), (3), (3), (2), (1), (1), (1)
Host Rabbit
(221), (93), (20), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(280), (144), (115), (86), (83), (53), (33), (27), (26), (22), (7), (6), (1)
Pubmed 2 references available
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) Images
Blocking Peptide Blocking peptide for this product available: ABIN412827
Specificity Detects 43 kDa Mcl-1
Purification Affinity purified
Immunogen Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
Isotype IgG

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) Images back to top
Antigen
Alternative Name Mcl-1 (MCL1 Antibody Abstract)
Background Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
Synonyms: MCL1L, mcl1/EAT, MGC1839, MGC104264, TM, MCL1S, EAT
Gene ID 4170
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling References for anti-MCL-1 Antibody (ABIN222889) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipitation (10-20 μg/mL), and Immunohistochemistry (10-20 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details References for anti-MCL-1 Antibody (ABIN222889) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-MCL-1 Antibody (ABIN222889)

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
Product cited in:

Xu, Wells, Padilla, Kato, Kim, Lin: "A signaling pathway consisting of miR-551b, catalase and MUC1 contributes to acquired apoptosis resistance and chemoresistance." in: Carcinogenesis, Vol. 35, Issue 11, pp. 2457-66, 2014

Wang, Chen, Zeng, Bai, Tesfaigzi, Belinsky, Lin: "Akt-mediated eminent expression of c-FLIP and Mcl-1 confers acquired resistance to TRAIL-induced cytotoxicity to lung cancer cells." in: Molecular cancer therapeutics, Vol. 7, Issue 5, pp. 1156-63, 2008

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN222889) back to top
Supplier Images
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (ABIN222889) Western blot analysis of Mcl-1 in Ramos cell lysate.