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anti-Mouse (Murine) MCL-1 antibody for Immunohistochemistry (Frozen Sections)

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • bcl2-L-3
  • BCL2L3
  • EAT
  • mcl-1
  • Mcl-1
  • MCL1-ES
  • mcl1/EAT
  • MCL1L
  • MCL1S
  • TM
Epitope
AA 2-307
32
23
22
15
15
13
13
13
11
10
9
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
291
101
68
9
8
5
4
3
2
2
1
1
Host
Rabbit
204
76
18
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
11
9
9
6
6
5
3
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
244
117
102
57
55
31
25
22
21
7
7
6
1
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Catalog No. ABIN1869118
$ 396.00
Plus shipping costs $45.00

Similar anti-MCL-1 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 33 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 63 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Epitope AA 2-307
(32), (23), (22), (15), (15), (13), (13), (13), (11), (10), (9), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(291), (101), (68), (9), (8), (5), (4), (3), (2), (2), (1), (1)
Host Rabbit
(204), (76), (18), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(11), (9), (9), (6), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(244), (117), (102), (57), (55), (31), (25), (22), (21), (7), (7), (6), (1)
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FGLRRNAVI GLNLYCGGAS LGAGGGSPAG ARLVAEEAKA RREGGGEAAL LPGARVVARP PPVGAEDPDV TASAERRLHK SPGLLAVPPE EMAASAAAAI VSPEEELDGC EPEAIGKRPA VLPLLERVSE AAKSSGADGS LPSTPPPPEE EEDDLYRQSL EIISRYLREQ ATGSKDSKPL GEAGAAGRRA LETLRRVGDG VQRNHETAFQ GMLRKLDIKN EGDVKSFSRV MVHVFKDGVT NWGRIVTLIS FGAFVAKHLK SVNQESFIEP LAETITDVLV RTKRDWLVKQ RGWDGFVEFF HVQDLEG
Specificity It has been selected for its ability to recognize MCL1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen MCL1 (AA 2-307)
Isotype IgG

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) (MCL1 Antibody Abstract)
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant MCL1 (Phe2~Gly307) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (AA 2-307) (ABIN1869118) anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 2-307) antibody