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anti-Human RAF1 antibody for Enzyme Immunoassay

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • 6430402F14Rik
  • AA990557
  • Afr-1
  • Afr1
  • AFR1
  • BB129353
  • c-raf
  • c-Raf
  • CRAF
  • craf
  • Craf1
  • D830050J10Rik
  • mKIAA0854
  • NS5
  • Raf
  • raf
  • RAF
  • Raf-1
  • RAF1
  • raf1
  • v-Raf
Epitope
pSer296
74
62
56
39
37
33
27
27
26
26
23
23
21
20
19
17
17
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
791
338
322
18
18
10
5
4
4
2
2
2
1
1
Host
Rabbit
742
41
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
12
12
12
12
12
12
12
12
12
Application
Enzyme Immunoassay (EIA)
463
355
207
182
132
79
40
32
17
16
12
2
2
2
Supplier
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Catalog No. ABIN358264
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087859354 ABIN358313 EIA Rabbit Ig pThr269 Log in to see Polyclonal 2
0.00087859354 ABIN358378 EIA Rabbit Ig pSer259 Log in to see Polyclonal 5
0.00087859354 ABIN360145 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 2
0.00087859354 ABIN358302 EIA Rabbit Ig pSer471 Log in to see Polyclonal 5
0.00087859354 ABIN358300 EIA Rabbit Ig Ser43, pSer43 Log in to see Polyclonal 5
0.00087859354 ABIN358407 EIA Rabbit Ig pTyr341 Log in to see Polyclonal 5
0.00087859354 ABIN358442 EIA Rabbit Ig pSer339 Log in to see Polyclonal 2
0.00087859354 ABIN358392 DB EIA Rabbit Ig pSer494 Log in to see Polyclonal 5
0.00087859354 ABIN358380 EIA Rabbit Ig pTyr340 Log in to see Polyclonal 5
0.00087859354 ABIN781705 EIA WB Chicken N-Term Log in to see Polyclonal 2
0.00087859354 ABIN358317 EIA WB Rabbit Ig pSer338 Log in to see Polyclonal 2
0.00087859354 ABIN1108823 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 4G4 2
0.00087859354 ABIN126881 EIA WB Mouse IgG2a pSer621 Log in to see 6B4
0.00087859354 ABIN114553 EIA WB Mouse IgG1 Log in to see 410
0.00087859354 ABIN452648 EIA WB Mouse IgG2b N-Term Log in to see 163
0.00087859354 ABIN452646 EIA WB Mouse IgG1 N-Term Log in to see 540
0.00087859354 ABIN452647 EIA WB Mouse IgG1 N-Term Log in to see 863

Top referenced anti-RAF1 antibody for Enzyme Immunoassay

Similar anti-RAF1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 182 Antibodies
ELISA 353 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 31 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 132 Antibodies
Immunohistochemistry (IHC) 206 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 79 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 450 Antibodies

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Epitope pSer296
(74), (62), (56), (39), (37), (33), (27), (27), (26), (26), (23), (23), (21), (20), (19), (17), (17), (16), (16), (15), (15), (14), (13), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(791), (338), (322), (18), (18), (10), (5), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(742), (41), (16), (7), (2)
Clonality
Polyclonal
Conjugate This RAF1 antibody is un-conjugated
(43), (43), (42), (29), (29), (29), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application Enzyme Immunoassay (EIA)
(463), (355), (207), (182), (132), (79), (40), (32), (17), (16), (12), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN358264) Images
Specificity This antibody detects RAC1 pSer296.
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S296 of human RAF1.
Isotype Ig

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling References for anti-RAF1 Antibody (ABIN358264) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Molecular Weight 73052 Da
Gene ID 5894, 9606
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling References for anti-RAF1 Antibody (ABIN358264) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details References for anti-RAF1 Antibody (ABIN358264) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-RAF1 Antibody (ABIN358264)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Background publications

Dasgupta, Sun, Wang, Fusaro, Betts, Padmanabhan, Sebti, Chellappan: "Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9527-41, 2004

Ku, Fu, Omary: "Raf-1 activation disrupts its binding to keratins during cell stress." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 479-85, 2004

Akula, Ford, Whitman, Hamden, Shelton, McCubrey: "Raf promotes human herpesvirus-8 (HHV-8/KSHV) infection." in: Oncogene, Vol. 23, Issue 30, pp. 5227-41, 2004

Goetz, Harmon, ONeil, Burchill, Farrar: "STAT5 activation underlies IL7 receptor-dependent B cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 8, pp. 4770-8, 2004

Rashdan, Fogel, McHenry, Sherman, Temkit, Lehman: "Improved stent characteristics for prophylaxis of post-ERCP pancreatitis." in: Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, Vol. 2, Issue 4, pp. 322-9, 2004

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling References for anti-RAF1 Antibody (ABIN358264) back to top
Supplier Images
 image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer296) (ABIN358264) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer296) antibody