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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Cow (Bovine)||Dog (Canine)||Xenopus laevis||Non-Human Primate|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies||6 Antibodies||6 Antibodies||1 Antibodies||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Western Blotting (WB)||464 Antibodies||256 Antibodies||234 Antibodies||24 Antibodies||16 Antibodies||22 Antibodies||8 Antibodies||4 Antibodies|
|RNA Interference (RNAi)||2 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Antigen||V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This RAF1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-RAF1 AntibodyTarget Details RAF1 Application Details Handling Images
|Specificity||This antibody recognizes the ~74k Raf-1 protein phosphorylated at Ser301. Immunolabeling of the Raf-1 protein band is blocked by l-phosphatase treatment.|
Species reactivity (expected):Human, Bovine, Canine, Mouse, Xenopus and non-Human Primates.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf1.|
Target Details RAF1Product Details anti-RAF1 Antibody Application Details Handling Images back to top
|Alternative Name||RAF1 (RAF1 Antibody Abstract)|
|Background||The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf|
|Research Area||Kinases/Phosphatases, Signaling|
|Pathways||MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-RAF1 Antibody Target Details RAF1 Handling Images back to top
Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RAF1 Antibody Target Details RAF1 Application Details Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|