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|Antigen||V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) ELISA Kits|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Supplier||Log in to see|
Product Details RAF1 ELISA KitTarget details Application Details Handling Images
|Purpose||Human/Mouse/Rat Phospho-Raf-1 (Ser301) and Total Raf-1 ELISA Kit. This assay semi-quantitatively measures phophorylated Raf-1 (Ser301) and Total Raf-1 in lysate samples.|
|Sample Type||Cell Lysate, Tissue Lysate|
|Specificity||The antibody pair provided in this kit recognizes human, mouse, and rat Raf-1 phosphorylated at site Serine-301 and Total Raf-1.|
|Material not included||
Target detailsProduct Details RAF1 ELISA Kit Application Details Handling Images back to top
|Alternative Name||Raf-1 (RAF1 ELISA Kit Abstract)|
|Background||RAF proto-oncogene serine/threonine-protein kinase (Raf-1) phosphorylated at Serine-301 and Total 301|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details RAF1 ELISA Kit Target details Handling Images back to top
|Sample Volume||100 μL|
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
|Restrictions||For Research Use only|
HandlingProduct Details RAF1 ELISA Kit Target details Application Details Images back to top
|Storage Comment||Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.|
|Expiry Date||6 months|