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Mouse (Murine) RAF1 ELISA Kit

Recommended RAF1 Product (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) ELISA Kits
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Epitope
pSer301, total
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
20
17
17
1
1
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
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Catalog No. ABIN2748581
$ 628.10
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2748582 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN1380296 Cell ELISA Log in to see
1 ABIN1380258 Cell ELISA Log in to see
1 ABIN1380400 Cell ELISA Log in to see
1 ABIN1380689 Cell ELISA Log in to see
1 ABIN1380880 Cell ELISA Log in to see
1 ABIN1380259 Cell ELISA Log in to see
1 ABIN1381048 Cell ELISA Log in to see
1 ABIN2113251 Cell ELISA Log in to see
1 ABIN2113249 Cell ELISA Log in to see
1 ABIN2113248 Cell ELISA Log in to see
1 ABIN2113253 Cell ELISA Log in to see
1 ABIN2113252 Cell ELISA Log in to see
1 ABIN2113250 Cell ELISA Log in to see
1 ABIN2113247 Cell ELISA Log in to see
1 ABIN2685342 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN1154353 Log in to see

Similar RAF1 ELISA Kits

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
ELISA 21 ELISA Kits 18 ELISA Kits 18 ELISA Kits

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) ELISA Kits
Epitope pSer301, total
Reactivity Human, Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
(20), (17), (17), (1), (1)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details RAF1 ELISA Kit

Target details Application Details Handling Images
Purpose Human/Mouse/Rat Phospho-Raf-1 (Ser301) and Total Raf-1 ELISA Kit. This assay semi-quantitatively measures phophorylated Raf-1 (Ser301) and Total Raf-1 in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes human, mouse, and rat Raf-1 phosphorylated at site Serine-301 and Total Raf-1.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details RAF1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Raf-1 (RAF1 ELISA Kit Abstract)
Background RAF proto-oncogene serine/threonine-protein kinase (Raf-1) phosphorylated at Serine-301 and Total 301
Gene ID 5894
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C

Application Details

Product Details RAF1 ELISA Kit Target details Handling Images back to top
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details RAF1 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months