You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human RPS6KA1 antibody for Western Blotting

Recommended RPS6KA1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
  • CLS
  • hu-1
  • HU-1
  • HU-3
  • ISPK-1
  • mapkapk1a
  • MAPKAPK1A
  • MAPKAPK1B
  • MRX19
  • p90-RSK2
  • p90rsk
  • pp90RSK2
  • RPS6KA
  • rsk
  • RSK
  • RSK1
  • rsk1
  • Rsk1
  • RSK2
  • S6K-alpha3
Epitope
C-Term
66
42
33
25
23
18
15
12
10
9
8
8
7
6
6
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Horse (Equine), Cow (Bovine), Dog (Canine)
320
149
118
28
10
7
5
2
2
1
Host
Rabbit
317
41
Clonality
Polyclonal
Conjugate
This RPS6KA1 antibody is un-conjugated
20
19
17
12
12
12
8
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
249
158
99
58
53
40
34
20
11
7
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1043905
$ 135.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN1043904 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.124960825 ABIN954622 EIA IHC (p) WB Rabbit Ig Log in to see Polyclonal 1
0.124960825 ABIN361702 IP ELISA WB Rabbit C-Term Log in to see Polyclonal 4
0.124960825 ABIN1881765 DB WB Rabbit pThr359 Log in to see Polyclonal 5
0.124960825 ABIN967838 IF IP IHC WB Mouse IgG2a AA 1-184 Log in to see 78-RSK 5
0.124960825 ABIN967837 IF IP IHC WB Mouse IgG2a AA 1-184 Log in to see 78-RSK 5
0.124960825 ABIN964912 ELISA WB Rabbit IgG pSer732, C-Term Log in to see Polyclonal
0.124960825 ABIN1724669 IHC ELISA WB Mouse IgG2b Log in to see 10B1D7A9 2
0.124960825 ABIN392452 IHC (p) WB Rabbit Ig AA 710-735, Ser732 Log in to see Polyclonal
0.124960825 ABIN500636 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.124960825 ABIN615401 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.124960825 ABIN3042929 WB Rabbit IgG AA 721-735, C-Term Log in to see Polyclonal
0.124960825 ABIN2856202 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
0.124960825 ABIN349634 ELISA WB Rabbit IgG C-Term, pSer732 Log in to see Polyclonal
0.124960825 ABIN498221 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.124960825 ABIN659087 WB Mouse IgG1, kappa Log in to see 205CT18-3-1
0.124960825 ABIN1108902 EIA IHC (p) WB Mouse IgG2a Log in to see 10B1D7A9 3
0.124960825 ABIN654115 IHC (p) WB Rabbit Ig Log in to see Polyclonal
0.124960825 ABIN1870594 IF IHC WB Rabbit IgG pThr348 Log in to see Polyclonal
0.124960825 ABIN1870596 WB Rabbit IgG pSer352 Log in to see Polyclonal

Top referenced anti-RPS6KA1 antibody for Western Blotting

Similar anti-RPS6KA1 Antibodies

Application / Reactivity Cow (Bovine) Human Mouse (Murine) Rat (Rattus) Dog (Canine) Horse (Equine)
Western Blotting (WB) 28 Antibodies 224 Antibodies 102 Antibodies 74 Antibodies 10 Antibodies 6 Antibodies
Immunoprecipitation (IP) 20 Antibodies 39 Antibodies 24 Antibodies 25 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 49 Antibodies 21 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 92 Antibodies 23 Antibodies 21 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) Antibodies
Epitope C-Term
(66), (42), (33), (25), (23), (18), (15), (12), (10), (9), (8), (8), (7), (6), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Horse (Equine), Cow (Bovine), Dog (Canine)
(320), (149), (118), (28), (10), (7), (5), (2), (2), (1)
Host Rabbit
(317), (41)
Clonality
Polyclonal
Conjugate This RPS6KA1 antibody is un-conjugated
(20), (19), (17), (12), (12), (12), (8), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(249), (158), (99), (58), (53), (40), (34), (20), (11), (7), (2), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-RPS6KA1 Antibody

Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN1043905) Images
Specificity Anti-p90 RSK1 was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
Characteristics Ribosomal S6 Kinase 1 (RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
Sterility Sterile filtered
Immunogen Anti-p90 RSK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Immunogen Type: Peptide
Isotype IgG

Target Details RPS6KA1

Product Details anti-RPS6KA1 Antibody Application Details Handling References for anti-RPS6KA1 Antibody (ABIN1043905) Images back to top
Antigen
Alternative Name p90 RSK1 (RPS6KA1 Antibody Abstract)
Background Ribosomal S6 Kinase 1 (RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
Synonyms: p90 RSK1, p90-RSK 1, p90RSK1, p90S6K, Ribosomal S6 Kinase 1, RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, MAPKAPK1A, MAPKAP kinase 1a, MAPKAPK-1a
Gene ID 6195
NCBI Accession NP_001006666
UniProt Q15418
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Handling References for anti-RPS6KA1 Antibody (ABIN1043905) Images back to top
Application Notes Anti-p90 RSK1 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 protein is expected in the appropriate cell lysate or extract.
Comment

Gene Name: RPS6KA1

Restrictions For Research Use only

Handling

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details References for anti-RPS6KA1 Antibody (ABIN1043905) Images back to top
Format Liquid
Concentration 1.51 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-RPS6KA1 Antibody (ABIN1043905)

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling Images back to top
Product cited in:

Chaturvedi, Poppleton, Stringfield, Barbier, Patel: "Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4586-600, 2006

Images

Product Details anti-RPS6KA1 Antibody Target Details RPS6KA1 Application Details Handling References for anti-RPS6KA1 Antibody (ABIN1043905) back to top
Supplier Images
Western Blotting (WB) image for anti-RPS6KA1 antibody (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1) (C-Term) (ABIN1043905) Western Blot of Rabbit anti-p90 RSK1 antibody. Lane 1: unstimulated HEK293T cell lysa...