|Application / Reactivity||Rat (Rattus)||Human|
|BioImaging (BI)||1 Antibodies||1 Antibodies|
|ELISA||60 Antibodies||102 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||14 Antibodies||18 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies|
|Immunofluorescence (IF)||43 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||41 Antibodies|
|Immunohistochemistry (IHC)||130 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||65 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||169 Antibodies|
|Biochemical Assay (BCA)||2 Antibodies|
|Antigen||Stathmin 1 (STMN1) Antibodies|
|Epitope||AA 38-147 Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This Stathmin 1 antibody is un-conjugated Alternatives|
BioImaging (BI), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-Stathmin 1 AntibodyTarget Details Stathmin 1 Application Details Handling References for anti-Stathmin 1 Antibody (ABIN968392) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human Metablastin aa. 38-147|
Target Details Stathmin 1Product Details anti-Stathmin 1 Antibody Application Details Handling References for anti-Stathmin 1 Antibody (ABIN968392) Images back to top
|Alternative Name||Metablastin (STMN1 Antibody Abstract)|
The regulation of microtubule (MT) assembly is vital to cellular processes such as organelle transport, organization of the cytoplasm, and intracellular movement of cell surface receptors. MTs are composed of tubulin subunits that exist in dynamic equilibrium between free tubulin dimers and MTs. The instability of MTs is determined by the rates of growth and shrinkage of tubulin polymers and by frequencies of transitions from growth to shrinkage (catastrophes) or from shrinkage to growth (rescues). The most well known MT regulators are the microtubule associated proteins (MAPs) which directly bind and stabilize MTs. Metablastin (stathmin) opposes MAP activity by inducing catastrophes.Metablastin is variably phosphorylated on multiple Ser residues by kinases that are regulated by the cell cycle or by external signals. Phosphorylation of metablastin inhibits its ability to destabilize MTs and, in turn, induces tubulin polymerization. Metablastin activity is turned off during the cell cycle to allow spindle formation and cell division. Thus, metablastin is thought to function to regulate the dynamics of MT formation in response to external signals during the interphase of the cell cycle.
Synonyms: Stathmin, Metablastin
|Molecular Weight||19 kDa|
|Pathways||MAPK Signaling, Microtubule Dynamics|
Application DetailsProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Handling References for anti-Stathmin 1 Antibody (ABIN968392) Images back to top
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Related Products: ABIN967389, ABIN968537
|Restrictions||For Research Use only|
HandlingProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details References for anti-Stathmin 1 Antibody (ABIN968392) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-Stathmin 1 Antibody (ABIN968392)Product Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Handling Images back to top
|Product cited in:||
Gradin, Larsson, Marklund, Gullberg: "Regulation of microtubule dynamics by extracellular signals: cAMP-dependent protein kinase switches off the activity of oncoprotein 18 in intact cells." in: The Journal of cell biology, Vol. 140, Issue 1, pp. 131-41, 1998
Horwitz, Shen, He, Dittmar, Neef, Chen, Schubart: "The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8129-32, 1997
Larsson, Marklund, Gradin, Brattsand, Gullberg: "Control of microtubule dynamics by oncoprotein 18: dissection of the regulatory role of multisite phosphorylation during mitosis." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5530-9, 1997
ImagesProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Handling References for anti-Stathmin 1 Antibody (ABIN968392) back to top