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|Antigen||Stathmin 1 (STMN1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Stathmin 1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Stathmin 1 AntibodyTarget Details Stathmin 1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Op18 protein only when phosphorylated at Ser16.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details Stathmin 1Product Details anti-Stathmin 1 Antibody Application Details Handling Images back to top
|Alternative Name||Stathmin / STMN1 (STMN1 Antibody Abstract)|
|Background||Oncoprotein 18 (Op18) is a regulator of microtubule (MT) dynamics. Op18 is a target for both cell cycle and cell surface receptor-coupled kinase systems, and phosphorylation of Op18 on specific combinations of amino acid residues has been shown to switch off MT-destabilizing activity of Op18. Induced expression of the catalytic subunit of cAMPdependent protein kinase (PKA) results in a dramatic increase in cellular MT polymer content concomitant with phosphorylation and partial degradation of Op18. Phosphorylation of Op18 on two specific sites, Serine 16 and Serine 63, is necessary and sufficient for PKA to switch off Op18 activity in intact cells. PKA phosphorylation down-regulates the MT-destabilizing activity of Op18, which in turn promotes increased Tubulin polymerization. Op18 has the potential to regulate the MT system in response to external signals such as cAMP-linked agonists.Synonyms: LAP18, Leukemia-associated phosphoprotein p18, Metablastin, OP18, Oncoprotein 18, Op18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, pp19|
|Molecular Weight||approx. 19 kDa|
|Pathways||MAPK Signaling, Microtubule Dynamics|
Application DetailsProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Stathmin 1 Antibody Target Details Stathmin 1 Application Details Handling back to top