100bp DNA Ladder

Details for Product No. ABIN1540468, Supplier: Log in to see
Detection Range
100-1500 bp
Minimum Detection Limit
100 bp
Application
Agarose Gel Electrophoresis (AGE)
Options
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Purpose 100bp DNA Ladder (Quantitative) is ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs.
Detection Method Fluorometric
Characteristics The ladder consists of 11 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 500bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 500bp are at 40ng. The 500bp fragment is at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
11 DNA Fragments (bp): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, and 1500
500bp Band: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 100bp-1.5kb
Bands: 11
Application Notes Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 1.7% Agarose Gel, 0.5×TBE, 5V/cm, 1h.
Comment

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Restrictions For Research Use only
Format Liquid
Storage -20 °C
Storage Comment 1 year at room temperature, for long term storage, please keep at -20°C.
Expiry Date 12 months
Supplier Images
Agarose Gel Electrophoresis (AGE) image for 100bp DNA Ladder (ABIN1540468) 100bp DNA Ladder