BLUelf Prestained Protein Ladder

Details for Product No. ABIN2868250, Supplier: Log in to see
Detection Range
3.5-245 kDa
Minimum Detection Limit
3.5 kDa
Application
SDS-PAGE (SDS), Western Blotting (WB)
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Detection Method Colorimetric
Application Notes Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.
Comment

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitroce-llulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

Reagent Preparation

Quality Control:
Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.

Restrictions For Research Use only
Format Liquid
Buffer Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM ~3.5 ~5 Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Bis-Tris 10 % Tris-Glycine Urea, and 15 % (v/v) Glycerol). MES buffer 4~20 %
Storage RT
Storage Comment Store at 25°C for 6 months, Store at -20°C for 24 months
Expiry Date 24 months
Supplier Images
 image for BLUelf Prestained Protein Ladder (ABIN2868250) Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis c...