Human ROCK1 ELISA Kit

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Antigen
rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) ELISA Kits
  • LOC100229794
  • CG9774
  • DROK
  • DRhk
  • DRok
  • Dmel\\CG9774
  • Drok
  • ROCK
  • ROCK-1
  • ROCK1
  • ROK
  • ROKalpha/beta
  • Rhk
  • RhoK
  • Rock
  • Rok
  • dROK
  • dRok
  • drok
  • 1110055K06Rik
  • Rock-I
  • P160Rock
  • ROCK-I
  • P160ROCK
  • p160ROCK
  • Rho associated coiled-coil containing protein kinase 1
  • Rho kinase
  • Rho-associated coiled-coil containing protein kinase 1
  • Rho-associated, coiled-coil containing protein kinase 1
  • ROCK1
  • Rok
  • Rock1
  • rock1
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
3
Method Type
Sandwich ELISA
Detection Range
0.156-10 ng/mL
Minimum Detection Limit
0.156 ng/mL
Application
ELISA
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Catalog No. ABIN5608555
$ 870.62
Plus shipping costs $45.00

General

Antigen rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(3)
Method Type Sandwich ELISA
Detection Range 0.156-10 ng/mL
Minimum Detection Limit 0.156 ng/mL
Application ELISA
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Product Details ROCK1 ELISA Kit

Target Details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Rock1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Sensitivity 0.048 ng/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target Details

Product Details ROCK1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1) (ROCK1 ELISA Kit Abstract)
Gene ID 6093
UniProt Q13464
Pathways Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling

Application Details

Product Details ROCK1 ELISA Kit Target Details Handling Images back to top
Assay Time 1 - 4.5 h
Plate Pre-coated
Assay Procedure The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

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Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Expiry Date 12 months

Images

Product Details ROCK1 ELISA Kit Target Details Application Details Handling back to top
Supplier Images
ELISA image for rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) ELISA Kit (ABIN5608555) Typical Standard Curve