We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Tubulin, Beta, 3 (TUBB3) Antibodies|
|Epitope||AA 441-448 Alternatives|
|Reactivity||All Species Alternatives|
|Conjugate||This TUBB3 antibody is conjugated to FITC Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-TUBB3 AntibodyTarget Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN269248) Images
|Specificity||Class III beta-tubulin specific for neurones.|
|Cross-Reactivity (Details)||Not yet tested in other species.|
|Immunogen||Synthetic peptide: ESESQGPK conjugated to malemide activated KLH, corresponding to amino acids 441-448 of Human Tubulin (NM_001197181.1).|
Target Details TUBB3Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN269248) Images back to top
|Alternative Name||TUBB3 / TUBB4 (TUBB3 Antibody Abstract)|
Tubulin is the major building block of microtubules. This intracellular cylindricalfilamentous structure is present in almost all eukaryotic cells. Microtubules function asstructural and mobile elements in mitosis, intracellular transport, flagellar movement,and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as amixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Withineither set of polypeptides, individual subunits diverge from each other (both within andacross species) at less than 10% of the amino acid positions. The most extremediversity is localized to the 15 residues of the carboxy terminal. For beta tubulin fiveevolutionarily conserved isotype clones have been identified. These are almost totallyconserved in the subunits utilized in the same cell types of different species, with theexception of the hematopoietic beta tubulin which is the most highly divergent insequence and is not conserved between species. Research has been centered aroundthe hypothesis that these beta tubulin isotypes contribute to unique functionalproperties. It has been reported that the different isotypes of tubulin differ from eachother in their ability to polymerize into microtubules. The monoclonal antibody fromhybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted withtubulin, tau or MAP2. Alternate Names: anti-M(beta)3 antibody, anti-M(beta)6 antibody, anti-MC1R antibody, anti-TUBB4antibody, anti-Tubulin beta 3 antibody, anti-Tubulin beta 4 antibody.
Gene Symbol: TUBB3
Application DetailsProduct Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN269248) Images back to top
For Immunohistochemistry-Paraffin: Use standard ABC technique (DAB+).
Recommended dilutions: Flow Cytometry 1:10-1:1000, Western Blot 1-2 µg/mL, Immunohistochemistry-Paraffin 10 µg/mL, Immunocytochemistry/Immunofluorescence 1:40, Immunohistochemistry 1:10-1:2000
|Restrictions||For Research Use only|
HandlingProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN269248) Images back to top
|Buffer||PBS. pH 7.4, Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Storage Comment||4 °C in the dark|
References for anti-TUBB3 Antibody (ABIN269248)Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
|Product cited in:||
Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).
Dráberová, Del Valle, Gordon et al.: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).
Katsetos, Dráberová, Smejkalová et al.: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).
Kukharskyy, Sulimenko, Mac?rek et al.: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).
Zíková, Sulimenko, Dráber et al.: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).
Dráberová, Lukás, Ivanyi et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).
ImagesProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN269248) back to top