|Application / Reactivity||Human||Chicken||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Fish||Mammalian||Pig (Porcine)||Baboon|
|Western Blotting (WB)||158 Antibodies||2 Antibodies||45 Antibodies||50 Antibodies||8 Antibodies||1 Antibodies||1 Antibodies||4 Antibodies||2 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||18 Antibodies||7 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||1 Antibodies||4 Antibodies||1 Antibodies||29 Antibodies|
|Antigen||Tubulin, Beta, 3 (TUBB3) Antibodies|
|Epitope||AA 441-448 Alternatives|
|Reactivity||Cow (Bovine), Chicken, Fish, Human, Mammalian, Mouse (Murine), Pig (Porcine), Rat (Rattus), Baboon Alternatives|
|Conjugate||This TUBB3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-TUBB3 AntibodyTarget Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN153066) Images
|Specificity||Class III beta-tubulin specific for neurones.|
|Cross-Reactivity (Details)||Not yet tested in other species.|
|Immunogen||Synthetic peptide: ESESQGPK conjugated to malemide activated KLH, corresponding to amino acids 441-448 of Human Tubulin (NM_001197181.1).|
Target Details TUBB3Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN153066) Images back to top
|Alternative Name||TUBB3 / TUBB4 (TUBB3 Antibody Abstract)|
Beta III tubulin is abundant in the central and peripheral nervous systems (CNS andPNS) where it is prominently expressed during fetal and postnatal development. Asexemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta IIIis neuron-associated, exhibiting distinct temporospatial gradients according to theregional neuroepithelia of origin. However, transient expression of this protein is alsopresent in the subventricular zones of the CNS comprising putative neuronal- and/orglial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratoryepithelium. This temporally restricted, potentially non-neuronal expression may haveimplications in the identification of presumptive neurons derived from embryonic stemcells. In adult tissues, the distribution of beta III is almost exclusively neuron-specific. Alternate Names: anti-Alpha tubulin ubiquitous antibody, anti-H2 ALPHA antibody, anti-TUBA1 antibody,anti-Tubulin alpha 1 chain antibody, anti-Tubulin alpha antibody, anti-Tubulin alpha ubiquitous chain antibody, anti-Tubulin K alpha 1 antibody.
Gene Symbol: TUBB3
|Gene ID||10381, 22142, 64158|
Application DetailsProduct Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN153066) Images back to top
For Immunohistochemistry-Paraffin: Use standard ABC technique (DAB+).
Recommended dilutions: Flow Cytometry 1:10-1:1000, Western Blot 1-2 µg/mL, Immunohistochemistry-Paraffin 10 µg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500
|Restrictions||For Research Use only|
HandlingProduct Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN153066) Images back to top
|Buffer||PBS [pH 7.4], Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||4 °C short term. Aliquot and store at -20 °C long term.|
References for anti-TUBB3 Antibody (ABIN153066)Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
|Product cited in:||
Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).
Dráberová, Del Valle, Gordon et al.: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).
Katsetos, Dráberová, Smejkalová et al.: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).
Kukharskyy, Sulimenko, Mac?rek et al.: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).
Zíková, Sulimenko, Dráber et al.: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).
Dráberová, Lukás, Ivanyi et al.: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).
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