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anti-Human TUBB3 antibody for Immunohistochemistry

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • 3200002H15Rik
  • AI325297
  • beta-4
  • CDCBM
  • CFEOM3A
  • M(beta)3
  • M(beta)4
  • M(beta)6
  • Tubb
  • tubb3
  • TUBB3
  • Tubb4
  • TUBB4
Epitope
AA 443-450
34
27
11
7
5
5
4
4
4
4
3
3
2
2
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
188
54
51
11
4
4
4
2
2
2
1
1
Host
Rabbit
100
90
14
12
1
Clonality
Polyclonal
Conjugate
This TUBB3 antibody is un-conjugated
23
12
11
3
3
3
3
2
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
177
80
79
44
42
35
33
15
13
9
6
2
2
2
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN1742553
$ 358.05
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22915025 ABIN1574042 FACS IHC ELISA WB Rabbit IgG AA 150-200 Log in to see Polyclonal 4
0.22915025 ABIN258508 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP1331Y
0.22915025 ABIN258509 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see EP1569Y
0.22915025 ABIN1742554 ICC IP IHC WB Guinea Pig AA 443-450 Log in to see Polyclonal 2
0.22915025 ABIN2500820 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.22915025 ABIN2466915 ICC IHC WB Chicken IgY Log in to see Polyclonal
0.22915025 ABIN2420885 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN2498123 IHC ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.0011319318 ABIN1875231 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN1945703 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 43-71 Log in to see Polyclonal
0.0011319318 ABIN1945704 FACS IHC ELISA WB APC Rabbit IgG N-Term, AA 43-71 Log in to see Polyclonal
0.0011319318 ABIN1945705 FACS IHC ELISA WB PE Rabbit IgG N-Term, AA 43-71 Log in to see Polyclonal
0.0011319318 ABIN355414 IHC ELISA WB Rabbit IgG AA 150-200 Log in to see Polyclonal
0.0011319318 ABIN3021273 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN151159 ICC IF IHC IHC (wm) WB Chicken Log in to see Polyclonal
0.0011319318 ABIN602477 IHC ELISA WB Mouse IgG1 Log in to see
0.0011319318 ABIN1839149 FACS IHC WB Rabbit N-Term, AA 36-63 Log in to see Polyclonal
0.0011319318 ABIN2737781 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN2380103 IC FACS IP IHC WB Rabbit IgG C-Term Log in to see 9i224
0.0011319318 ABIN932443 IHC WB Mouse IgG2a Log in to see TUJ-1

Top referenced anti-TUBB3 antibody for Immunohistochemistry

Similar anti-TUBB3 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 8 Antibodies 10 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies 12 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 49 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope AA 443-450
(34), (27), (11), (7), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Reactivity Human, Rat (Rattus), Mouse (Murine)
(188), (54), (51), (11), (4), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(100), (90), (14), (12), (1)
Clonality
Polyclonal
Conjugate This TUBB3 antibody is un-conjugated
(23), (12), (11), (3), (3), (3), (3), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(177), (80), (79), (44), (42), (35), (33), (15), (13), (9), (6), (2), (2), (2)
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling Images
Specificity Specific for beta3-tubulin.
Purification antiserum
Immunogen Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name beta3-Tubulin (TUBB3) (TUBB3 Antibody Abstract)
Background Synonyms: TuJ1
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling Images back to top
Application Notes WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 1000 up to 1 : 5000
IHC: 1 : 200 up to 1 : 500
Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Images back to top
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 443-450) (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody
Immunocytochemistry (ICC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 443-450) (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 443-450) (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (Image 3)
'Independent Validation' Badge
Lot Number 302302/3
Method validated Western Blotting
Positive Control Mouse brain
Negative Control Mouse heart, HeLa cells, A431 cells
Notes A band was observed in the positive controls at the expected size (~50 kDa), which is lower in the negative controls.
Primary Antibody
  • Antigen: Tubulin, Beta, 3 (TUBB3) (AA 443-450)
  • Catalog number: ABIN1742553
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  • Supplier catalog number: Log in to see
  • Lot number: 302302/3
  • Dilution: 1:1000
Secondary Antibody
  • Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
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  • Lot number: F0613
  • Dilution: 1:10,000
Controls - Lysates were prepared by Kinexus Bioinformatics Corporation following standard protocols and quality controlled for protein integrity on a regular basis.
Protocol
  • 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
  • 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
  • 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
  • 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
  • 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
  • 6. The membrane was washed in TTBS twice for 5 minutes each.
  • 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
  • 8. The membrane was rinsed twice with TTBS.
  • 9. The membrane was washed in TTBS twice for 5 minutes each.
  • 10. The membrane was washed in TTBS once for 15 minutes.
  • 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
  • 12. The membrane was rinsed twice with TTBS.
  • 13. The membrane was washed in TTBS twice for 5 minutes each.
  • 14. The membrane was washed in TTBS once for 15 minutes.
  • 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
  • 16. The membrane was rinsed three times with TTBS.
  • 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (ABIN1742553) Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the exp...