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anti-Mouse (Murine) TUBB3 antibody for ELISA

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • 3200002H15Rik
  • AI325297
  • beta-4
  • CDCBM
  • CFEOM3A
  • M(beta)3
  • M(beta)4
  • M(beta)6
  • Tubb
  • tubb3
  • TUBB3
  • Tubb4
  • TUBB4
Epitope
AA 150-200
34
27
11
7
5
5
5
4
4
3
3
3
2
2
1
Reactivity
Cynomolgus, Cow (Bovine), Chicken, Human, Rat (Rattus), Mouse (Murine)
188
54
51
10
4
4
4
2
2
2
Host
Rabbit
100
90
14
12
1
Clonality
Polyclonal
Conjugate
This TUBB3 antibody is un-conjugated
23
12
11
3
3
3
3
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
177
79
79
43
42
36
33
16
13
9
6
2
2
2
Supplier
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Catalog No. ABIN1574042
$ 147.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012092679 ABIN265869 ELISA WB Mouse IgG2b C-Term Log in to see SDL-3D10
0.0012092679 ABIN2500820 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012092679 ABIN2420885 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012092679 ABIN1094056 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012092679 ABIN1094057 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012092679 ABIN2498123 IHC ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.0012092679 ABIN3029345 FACS IHC ELISA WB Rabbit Ig Fraction AA 36-63 Log in to see Polyclonal
0.0012092679 ABIN2560604 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0012092679 ABIN1740450 ICC IHC ELISA WB Rabbit IgG AA 433-450 Log in to see Polyclonal

Top referenced anti-TUBB3 antibody for ELISA

Similar anti-TUBB3 Antibodies

Application / Reactivity Human Chicken Mouse (Murine) Rat (Rattus) Cow (Bovine) Cynomolgus
Western Blotting (WB) 157 Antibodies 1 Antibodies 47 Antibodies 49 Antibodies 7 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 18 Antibodies 7 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 28 Antibodies 14 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope AA 150-200
(34), (27), (11), (7), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1)
Reactivity Cynomolgus, Cow (Bovine), Chicken, Human, Rat (Rattus), Mouse (Murine)
(188), (54), (51), (10), (4), (4), (4), (2), (2), (2)
Host Rabbit
(100), (90), (14), (12), (1)
Clonality
Polyclonal
Conjugate This TUBB3 antibody is un-conjugated
(23), (12), (11), (3), (3), (3), (3), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(177), (79), (79), (43), (42), (36), (33), (16), (13), (9), (6), (2), (2), (2)
Pubmed 4 references available
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Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN1574042) Images
Specificity Rabbit Anti-beta-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse beta-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone beta-tubulin III proteins. Reactivity with other β-Tubulin had not been tested yet.
Cross-Reactivity (Details) Rabbit Anti-beta-Tubulin III Polyclonal Antibody detects endogenous levels of human, rat and mouse beta-tubulin III protein. Sequence homology predicts that it will also react with bovine, chicken, cynomolgus monkey. African clawed frog, and disc abalone beta-tubulin III proteins.
Purification Immunoaffinity chromatography
Immunogen Synthetic peptide (KLH-coupled) within AA 150-200 of human β-tubulin III protein
Isotype IgG

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN1574042) Images back to top
Antigen
Alternative Name beta-Tubulin III (TUBB3 Antibody Abstract)
Background Beta-Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. beta-Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.Rabbit Anti-beta-Tubulin III Polyclonal Antibody is developed in rabbit hosts using a KLH-coupled synthetic peptide within residues 150-200 of human beta-Tubulin III protein (Swiss Prot: Q13509).
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN1574042) Images back to top
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL. Western blot: 0.5-1 µg/mL. Immunohistochemistry: 5-10 µg/mL. Flow cytometry: 1-3 µg for 1 x 10E6 cells. Other applications: user-optimized.
Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN1574042) Images back to top
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-TUBB3 Antibody (ABIN1574042)

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
Product cited in:

Yang, Zhu, Chen, Ye, Zhao, Mou, Hu: "Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivo." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 3, pp. 657-66, 2013

Hasegawa, Ryu, Kaláb: "Chromosomal gain promotes formation of a steep RanGTP gradient that drives mitosis in aneuploid cells." in: The Journal of cell biology, Vol. 200, Issue 2, pp. 151-61, 2013

Das, Anczuków, Akerman, Krainer: "Oncogenic splicing factor SRSF1 is a critical transcriptional target of MYC." in: Cell reports, Vol. 1, Issue 2, pp. 110-7, 2012

Hua, Sahashi, Hung, Rigo, Passini, Bennett, Krainer: "Antisense correction of SMN2 splicing in the CNS rescues necrosis in a type III SMA mouse model." in: Genes & development, Vol. 24, Issue 15, pp. 1634-44, 2010

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN1574042) back to top
Supplier Images
Western Blotting (WB) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 150-200) (ABIN1574042) Lane 1: MarkerLane 2: Hela cell lysateLane 3: HEK293 cell lysateLane 4: Mouse brain t...
Immunohistochemistry (IHC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 150-200) (ABIN1574042) Immunohistochemistry analysis of human brain tissue slide (Paraffin embedded) using R...
Immunohistochemistry (IHC) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 150-200) (ABIN1574042) Immunohistochemistry negative control analysis of human brain tissue slide (Paraffin ...