You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) TUBB3 antibody for Flow Cytometry

Recommended TUBB3 Antibody (supplied by: Log in to see )

Antigen
Tubulin, Beta, 3 (TUBB3) Antibodies
  • tubb3
  • TUBB3
  • TUBB4
  • CDCBM
  • CFEOM3A
  • beta-4
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • tubulin, beta 3 class III
  • beta-tubulin 3
  • similar to tubulin, beta 3
  • tubulin, beta, 4
  • tubulin, beta 3
  • beta tubulin3
  • tubulin, beta 2C
  • tubulin, beta 4A class IVA
  • tubb3
  • TUBB3
  • tub3
  • TUBB2C
  • Tubb3
  • Tubb4a
Epitope
AA 441-448, C-Term, N-Term
49
43
20
15
12
6
5
4
4
4
4
3
3
2
2
1
1
1
Reactivity
All Species, Human, Mouse (Murine)
231
87
79
19
15
10
7
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
128
121
16
14
1
Clonality
Monoclonal
Conjugate
This TUBB3 antibody is un-conjugated
26
14
13
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
227
102
84
64
58
56
50
17
13
13
12
8
6
2
2
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN153066
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.107948 ABIN2657231 FACS Alexa Fluor 647 Mouse IgG2a Log in to see AA10 1
13.107948 ABIN4284545 FACS ICC IF IHC IP WB Mouse IgG2a Log in to see 2G10
13.107948 ABIN4284549 FACS ICC IF IHC IHC (p) WB PerCP Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
13.107948 ABIN4284548 FACS PE Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
13.107948 ABIN3029345 FACS IHC ELISA WB Rabbit Ig Fraction AA 36-63 Log in to see Polyclonal
1 ABIN651938 FACS IHC (p) WB Rabbit Ig AA 36-63, N-Term Log in to see Polyclonal 1
1 ABIN269248 ICC FACS IF IHC (p) IHC WB FITC Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20 10
1 ABIN1574042 FACS IHC ELISA WB Rabbit IgG AA 150-200 Log in to see Polyclonal 4
1 ABIN3021273 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2657232 FACS Alexa Fluor 647 Mouse IgG2a Log in to see AA10 1
1 ABIN4284558 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284547 FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284546 FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284551 FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284550 FACS Allophycocyanin Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284552 FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284553 FACS ICC IF IHC IHC (p) WB DyLight 488 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284556 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284559 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20
1 ABIN4284557 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Mouse IgG1 AA 441-448, C-Term, N-Term Log in to see TU-20

Top referenced anti-TUBB3 antibody for Flow Cytometry

Similar anti-TUBB3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 11 Antibodies 84 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Flow Cytometry (FACS) 23 Antibodies 54 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunohistochemistry (Whole Mount) (IHC (wm)) 2 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 65 Antibodies

General

Antigen Tubulin, Beta, 3 (TUBB3) Antibodies
Epitope AA 441-448, C-Term, N-Term
(49), (43), (20), (15), (12), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Reactivity All Species, Human, Mouse (Murine)
(231), (87), (79), (19), (15), (10), (7), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(128), (121), (16), (14), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TUBB3 antibody is un-conjugated
(26), (14), (13), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(227), (102), (84), (64), (58), (56), (50), (17), (13), (13), (12), (8), (6), (2), (2), (2), (2), (2)
Pubmed 9 references available
Supplier Log in to see

Product Details anti-TUBB3 Antibody

Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN153066) Images
Specificity The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Ammonium sulfate precipitation
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Clone TU-20
Isotype IgG1

Target Details TUBB3

Product Details anti-TUBB3 Antibody Application Details Handling References for anti-TUBB3 Antibody (ABIN153066) Images back to top
Antigen
Alternative Name TUBB3 / TUBB4 (TUBB3 Antibody Abstract)
Background Gene Symbol: TUBB3
Gene ID 10381
Pathways Microtubule Dynamics

Application Details

Product Details anti-TUBB3 Antibody Target Details TUBB3 Handling References for anti-TUBB3 Antibody (ABIN153066) Images back to top
Application Notes Western Blot 1-2 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 10 μg/mLWestern Blot - Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry-Paraffin sections - Staining technique: Standard ABC technique (DAB+) Pretreatment: 0.1 % pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT, or High temperature citrate buffer antigen retrieval
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details References for anti-TUBB3 Antibody (ABIN153066) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-TUBB3 Antibody (ABIN153066)

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling Images back to top
Product cited in:

Badizadegan, Thomas, Nagy, Ndishabandi, Miller, Alessandrini, Belkind-Gerson, Goldstein: "Presence of intramucosal neuroglial cells in normal and aganglionic human colon." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 307, Issue 10, pp. G1002-12, 2014 (Sample species: Human). Further details: Flow Cytometry

Kong, Wong, Chan, Lo: "Chronic exposure of adult rats to low doses of methylmercury induced a state of metabolic deficit in the somatosensory cortex." in: Journal of proteome research, Vol. 12, Issue 11, pp. 5233-45, 2013 (Sample species: Rat (Rattus)). Further details: Western Blotting

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012

Yoo, Kim, Nam, Chung, Choi, Yoon, Won, Hwang: "Chronic effects of pyridoxine in the gerbil hippocampal CA1 region after transient forebrain ischemia." in: Neurochemical research, Vol. 37, Issue 5, pp. 1011-8, 2012 Further details: Western Blotting,Immunohistochemistry

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998

Images

Product Details anti-TUBB3 Antibody Target Details TUBB3 Application Details Handling References for anti-TUBB3 Antibody (ABIN153066) back to top
Supplier Images
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [ABIN153066...
Flow Cytometry (FACS) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (AA 441-448) (ABIN153066) Flow Cytometry: beta-III Tubulin Antibody (TU-20) [ABIN153066] - Analysis of PerCP co...