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|Antigen||CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies|
|Epitope||AA 71-187 Alternatives|
|Conjugate||This CD40 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-CD40 AntibodyTarget Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN968494) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human CD40 aa. 71-187|
Target Details CD40Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN968494) Images back to top
|Alternative Name||CD40 (CD40 Antibody Abstract)|
|Background||CD40, a member of the TNF receptor family, contains a cysteine-rich N-terminal domain and a Ser/Thr-rich region preceding the transmembrane domain. On B cells, signaling through CD40 induces cell growth and differentiation, mediates cell survival within the germinal center, and upregulates the expression of costimulatory and adhesion molecules, such as B7.1, B7.2, and ICAM-1. The interaction of CD40 on B cells and CD40L on activated CD4+ T cells is essential for immune functions, such as immuoglobulin class switching. Signal transduction through CD40 pathways involves interaction with proteins such as TRAFs (TRAF2, TRAF3, TRAF5, and TRAF6), Jak 3, and Tyr phosphorylation of proteins, such as Lyn, Syk, PI-3-kinase, STAT3, and STAT5. In TRAF2-deficient mice, CD40-mediated B cell proliferation and NFkappaB activation are defective. Ku70 and Ku80 associate with the membrane-proximal region of CD40 in human primary B cells and the engagement of CD40 leads translocation of Ku proteins to the nucleus. Thus, CD40 interacts with a variety of signal transducers which mediate its role in B cell survival, growth, differentiation, and immunoglobulin class switching.|
|Molecular Weight||44 kDa|
|Research Area||Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer|
|Pathways||NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN968494) Images back to top
Related Products: ABIN968584, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN968494) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-CD40 antibody (ABIN968494)Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
|Product cited in:||
Morio, Hanissian, Bacharier, Teraoka, Nonoyama, Seki, Kondo, Nakano, Lee, Geha, Yata: "Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb." in: Immunity, Vol. 11, Issue 3, pp. 339-48, 1999
Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: "TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses." in: Immunity, Vol. 11, Issue 3, pp. 379-89, 1999
Randall, Heath, Santos-Argumedo, Howard, Weissman, Lund: "Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers." in: Immunity, Vol. 8, Issue 6, pp. 733-42, 1998
Stamenkovic, Clark, Seed: "A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas." in: The EMBO journal, Vol. 8, Issue 5, pp. 1403-10, 1989
ImagesProduct Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN968494) back to top