anti-Human CD40 antibody for Immunofluorescence

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • AI326936
  • Bp50
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • p50
  • CDW40
  • TNFRSF5
  • TNFSF5
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • CD40 antigen
  • CD40 molecule, TNF receptor superfamily member 5
  • dynactin 2
  • LOC100349106
  • Cd40
  • CD40
  • Dctn2
Epitope
AA 71-187
55
52
47
26
24
22
19
19
18
8
6
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
658
331
69
20
14
10
10
6
4
3
2
2
1
1
1
1
Host
Mouse
514
231
171
23
6
4
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
87
86
50
27
25
24
20
18
14
13
12
10
10
10
9
9
9
9
9
9
9
8
7
6
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
609
246
207
157
112
103
103
64
47
36
19
18
15
13
10
8
6
6
3
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN968495
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.945934 ABIN968494 IF WB Mouse IgG1 AA 71-187 Log in to see 41-CD40 4
11.945934 ABIN2704615 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.945934 ABIN1106353 EIA IF Mouse IgG1 Log in to see 9G10 3
11.945934 ABIN1997455 FACS IF IF/ICC Mouse IgG1 AA 1-193 Log in to see 17
11.945934 ABIN1997449 FACS IF IF/ICC Mouse AA 1-193 Log in to see 03
11.945934 ABIN4293032 ELISA ICC IF Mouse IgG1 Log in to see 9G10
11.945934 ABIN1326962 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 4F1
11.945934 ABIN1326964 IF ELISA WB Mouse IgG1 kappa AA 21-193, partial Log in to see 4F11
11.945934 ABIN1326967 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 5G10
11.945934 ABIN1326971 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 4G15
11.945934 ABIN931290 IF ELISA Mouse IgG1 Log in to see 9G10
1 ABIN1112133 FACS IF PE Mouse IgG1 Log in to see HI40a 1
1 ABIN2665694 FACS IF IHC Mouse IgG1 kappa Log in to see HB14 8
1 ABIN2659262 FACS IF PE-Cy7 Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN1112130 FACS IF APC Mouse IgG1 Log in to see HI40a 1
1 ABIN1112132 FACS IF FITC Mouse IgG1 Log in to see HI40a 1
1 ABIN467092 IF ELISA Mouse IgG1 Log in to see 9G10
1 ABIN1112134 FACS IF PerCP Mouse IgG1 Log in to see HI40a 1
1 ABIN2665695 FACS IF IHC Mouse IgG1 kappa Log in to see HB14 8
1 ABIN2851737 FACS IF PE-Cy5 Mouse IgG2b Log in to see

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 71-187
(55), (52), (47), (26), (24), (22), (19), (19), (18), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(658), (331), (69), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(514), (231), (171), (23), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(87), (86), (50), (27), (25), (24), (20), (18), (14), (13), (12), (10), (10), (10), (9), (9), (9), (9), (9), (9), (9), (8), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(609), (246), (207), (157), (112), (103), (103), (64), (47), (36), (19), (18), (15), (13), (10), (8), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN968495) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human CD40 aa. 71-187
Clone 41-CD40
Isotype IgG1

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 antibody (ABIN968495) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40, a member of the TNF receptor family, contains a cysteine-rich N-terminal domain and a Ser/Thr-rich region preceding the transmembrane domain. On B cells, signaling through CD40 induces cell growth and differentiation, mediates cell survival within the germinal center, and upregulates the expression of costimulatory and adhesion molecules, such as B7.1, B7.2, and ICAM-1. The interaction of CD40 on B cells and CD40L on activated CD4+ T cells is essential for immune functions, such as immuoglobulin class switching. Signal transduction through CD40 pathways involves interaction with proteins such as TRAFs (TRAF2, TRAF3, TRAF5, and TRAF6), Jak 3, and Tyr phosphorylation of proteins, such as Lyn, Syk, PI-3-kinase, STAT3, and STAT5. In TRAF2-deficient mice, CD40-mediated B cell proliferation and NFkappaB activation are defective. Ku70 and Ku80 associate with the membrane-proximal region of CD40 in human primary B cells and the engagement of CD40 leads translocation of Ku proteins to the nucleus. Thus, CD40 interacts with a variety of signal transducers which mediate its role in B cell survival, growth, differentiation, and immunoglobulin class switching.
Molecular Weight 44 kDa
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 antibody (ABIN968495) Images back to top
Comment

Related Products: ABIN968584, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 antibody (ABIN968495) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-CD40 antibody (ABIN968495)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Morio, Hanissian, Bacharier, Teraoka, Nonoyama, Seki, Kondo, Nakano, Lee, Geha, Yata: "Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb." in: Immunity, Vol. 11, Issue 3, pp. 339-48, 1999

Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: "TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses." in: Immunity, Vol. 11, Issue 3, pp. 379-89, 1999

Randall, Heath, Santos-Argumedo, Howard, Weissman, Lund: "Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers." in: Immunity, Vol. 8, Issue 6, pp. 733-42, 1998

Background publications

Stamenkovic, Clark, Seed: "A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas." in: The EMBO journal, Vol. 8, Issue 5, pp. 1403-10, 1989

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 antibody (ABIN968495) back to top
Supplier Images
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) Western blot analysis of CD40 on EB1 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lan...
Immunofluorescence (IF) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) Immunofluorescent staining of HeLa cells.
 image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (Image 3)
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (Image 4)