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anti-Human CD40 antibody for Immunofluorescence

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • 2310042E05Rik
  • AI326936
  • Bp50
  • C87049
  • C130077D06Rik
  • CDW40
  • DCTN-50
  • GMP23-48K
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • p50
  • RBP50
  • T-BAM
  • Tnfrsf5
  • TNFRSF5
  • TNFSF5
  • TRAP
Epitope
AA 71-187
61
37
31
28
22
19
15
8
7
7
5
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
605
302
57
13
11
8
7
5
4
2
1
1
1
1
1
Host
Mouse
481
211
154
24
9
2
Clonality
Monoclonal
Conjugate
This CD40 antibody is un-conjugated
95
91
52
24
20
17
14
12
12
11
9
8
8
8
8
8
8
8
8
8
7
6
6
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
567
199
198
107
88
78
72
49
48
21
19
11
8
8
6
4
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN968494
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN968495 IF WB Mouse IgG1 AA 71-187 Log in to see 41-CD40 4
0.32040194 ABIN1106353 EIA IF Mouse IgG1 Log in to see 9G10 3
0.32040194 ABIN2704615 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.32040194 ABIN931290 IF ELISA Mouse IgG1 Log in to see 9G10
0.32040194 ABIN1997455 FACS IF Mouse AA 1-193 Log in to see
0.32040194 ABIN1326964 IF ELISA WB Mouse IgG1 kappa AA 21-193, partial Log in to see 4F11
0.32040194 ABIN1326962 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 4F1
0.32040194 ABIN1326967 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 5G10
0.32040194 ABIN1326971 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 4G15
0.32040194 ABIN1997449 FACS IF Mouse AA 1-193 Log in to see
0.0010840179 ABIN1112130 FACS IF APC Mouse IgG1 Log in to see HI40a 1
0.0010840179 ABIN1112133 FACS IF PE Mouse IgG1 Log in to see HI40a 1
0.0010840179 ABIN1112132 FACS IF FITC Mouse IgG1 Log in to see HI40a 1
0.0010840179 ABIN2659262 FACS IF PE-Cy7 Mouse IgG1 kappa Log in to see 5C3 4
0.0010840179 ABIN2665694 FACS IF IHC Mouse IgG1 kappa Log in to see HB14 8
0.0010840179 ABIN467092 IF ELISA Mouse IgG1 Log in to see 9G10
0.0010840179 ABIN1106355 IHC (fro) IF Mouse IgM Log in to see BL-C4
0.0010840179 ABIN1112134 FACS IF PerCP Mouse IgG1 Log in to see HI40a 1
0.0010840179 ABIN2659263 FACS IF PE-Cy7 Mouse IgG1 kappa Log in to see 5C3 4
0.0010840179 ABIN2665695 FACS IF IHC Mouse IgG1 kappa Log in to see HB14 8

Top referenced anti-CD40 antibody for Immunofluorescence

  • Human Monoclonal CD40 Primary Antibody for IF, WB - ABIN968494 (4 Publications): Morio, Hanissian, Bacharier, Teraoka, Nonoyama, Seki, Kondo, Nakano, Lee, Geha, Yata: Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb. in Immunity 1999 (PubMed)

  • Human Monoclonal CD40 Primary Antibody for IF, WB - ABIN968495 (4 Publications): Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses. in Immunity 1999 (PubMed)

  • Human Monoclonal CD40 Primary Antibody for FACS, IF - ABIN1112130 (1 Publications): Fujiwara, Melenhorst, El Ouriaghli, Kajigaya, Grube, Sconocchia, Rezvani, Price, Hensel, Douek, Barrett: In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. in Clinical cancer research : an official journal of the American Association for Cancer Research 2005 (PubMed)

  • Human Monoclonal CD40 Primary Antibody for EIA, IF - ABIN1106353 (3 Publications): Linderoth, Jerkeman, Cavallin-Ståhl, Kvaløy, Torlakovic: Immunohistochemical expression of CD23 and CD40 may identify prognostically favorable subgroups of diffuse large B-cell lymphoma: a Nordic Lymphoma Group Study. in Clinical cancer research : an official journal of the American Association for Cancer Research 2003 (PubMed)

Similar anti-CD40 Antibodies

Application / Reactivity Human
ELISA 155 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 340 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 44 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 63 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 69 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 70 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 1 Antibodies
Immunoprecipitation (IP) 53 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Epitope AA 71-187
(61), (37), (31), (28), (22), (19), (15), (8), (7), (7), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(605), (302), (57), (13), (11), (8), (7), (5), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(481), (211), (154), (24), (9), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is un-conjugated
(95), (91), (52), (24), (20), (17), (14), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(567), (199), (198), (107), (88), (78), (72), (49), (48), (21), (19), (11), (8), (8), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN968494) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human CD40 aa. 71-187
Clone 41-CD40
Isotype IgG1

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN968494) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40, a member of the TNF receptor family, contains a cysteine-rich N-terminal domain and a Ser/Thr-rich region preceding the transmembrane domain. On B cells, signaling through CD40 induces cell growth and differentiation, mediates cell survival within the germinal center, and upregulates the expression of costimulatory and adhesion molecules, such as B7.1, B7.2, and ICAM-1. The interaction of CD40 on B cells and CD40L on activated CD4+ T cells is essential for immune functions, such as immuoglobulin class switching. Signal transduction through CD40 pathways involves interaction with proteins such as TRAFs (TRAF2, TRAF3, TRAF5, and TRAF6), Jak 3, and Tyr phosphorylation of proteins, such as Lyn, Syk, PI-3-kinase, STAT3, and STAT5. In TRAF2-deficient mice, CD40-mediated B cell proliferation and NFkappaB activation are defective. Ku70 and Ku80 associate with the membrane-proximal region of CD40 in human primary B cells and the engagement of CD40 leads translocation of Ku proteins to the nucleus. Thus, CD40 interacts with a variety of signal transducers which mediate its role in B cell survival, growth, differentiation, and immunoglobulin class switching.
Molecular Weight 44 kDa
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN968494) Images back to top
Comment

Related Products: ABIN968584, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN968494) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-CD40 Antibody (ABIN968494)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Product cited in:

Morio, Hanissian, Bacharier, Teraoka, Nonoyama, Seki, Kondo, Nakano, Lee, Geha, Yata: "Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb." in: Immunity, Vol. 11, Issue 3, pp. 339-48, 1999

Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: "TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses." in: Immunity, Vol. 11, Issue 3, pp. 379-89, 1999

Randall, Heath, Santos-Argumedo, Howard, Weissman, Lund: "Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers." in: Immunity, Vol. 8, Issue 6, pp. 733-42, 1998

Background publications

Stamenkovic, Clark, Seed: "A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas." in: The EMBO journal, Vol. 8, Issue 5, pp. 1403-10, 1989

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN968494) back to top
Supplier Images
Western Blotting (WB) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) (AA 71-187) (ABIN968494) Western blot analysis of CD40 on EB1 lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: ...
Immunofluorescence (IF) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) (AA 71-187) (ABIN968494) Immunofluorescent staining of HeLa cells.