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anti-Mouse (Murine) CD40 antibody for Intracellular Flow Cytometry

Recommended CD40 Antibody (supplied by: Log in to see )

Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
  • 2310042E05Rik
  • AI326936
  • Bp50
  • C87049
  • C130077D06Rik
  • CDW40
  • DCTN-50
  • GMP23-48K
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • p50
  • RBP50
  • T-BAM
  • Tnfrsf5
  • TNFRSF5
  • TNFSF5
  • TRAP
Reactivity
Mouse (Murine)
603
293
53
13
11
8
7
5
4
2
1
1
1
1
1
Host
Rat
476
205
157
24
4
3
Clonality
Monoclonal
Conjugate
This CD40 antibody is conjugated to Alexa Fluor 647
93
90
51
21
19
17
14
12
12
11
10
8
8
8
8
8
8
8
8
7
6
6
6
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
550
204
202
105
86
76
72
50
44
22
18
12
9
8
6
3
3
3
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN2657785
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008732642 ABIN2660110 ICFC PerCP-Cy5.5 Rat IgG2a kappa Log in to see 3-23 3
0.0008732642 ABIN2658659 ICFC APC Rat IgG2a kappa Log in to see 3-23 4
0.0008732642 ABIN2665701 ICFC Hamster IgM kappa Log in to see HM40-3 11
0.0008732642 ABIN2665696 ICFC Rat IgG2a kappa Log in to see 3-23 3
0.0008732642 ABIN2665993 IF ICFC IP IHC WB Rat IgG2a kappa Log in to see 3-23 3
0.0008732642 ABIN2657786 ICFC Alexa Fluor 647 Rat IgG2a kappa Log in to see 3-23 3
0.0008732642 ABIN2660111 ICFC PerCP-Cy5.5 Rat IgG2a kappa Log in to see 3-23 3
0.0008732642 ABIN2658660 ICFC APC Rat IgG2a kappa Log in to see 3-23 4
0.0008732642 ABIN2665697 ICFC Rat IgG2a kappa Log in to see 3-23 3
0.0008732642 ABIN2665700 ICFC Hamster IgM kappa Log in to see HM40-3 11

Top referenced anti-CD40 antibody for Intracellular Flow Cytometry

Similar anti-CD40 Antibodies

Application / Reactivity Mouse (Murine)
Activity Assay (AcA) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 51 Antibodies
ELISA (Capture) 9 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 214 Antibodies
Functional Studies (Func) 20 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 37 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies

General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antibodies
Reactivity Mouse (Murine)
(603), (293), (53), (13), (11), (8), (7), (5), (4), (2), (1), (1), (1), (1), (1)
Host Rat
(476), (205), (157), (24), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 antibody is conjugated to Alexa Fluor 647
(93), (90), (51), (21), (19), (17), (14), (12), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(550), (204), (202), (105), (86), (76), (72), (50), (44), (22), (18), (12), (9), (8), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD40 Antibody

Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2657785) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Clone 3-23
Isotype IgG2a kappa

Target Details CD40

Product Details anti-CD40 Antibody Application Details Handling References for anti-CD40 Antibody (ABIN2657785) Images back to top
Antigen
Alternative Name CD40 (CD40 Antibody Abstract)
Background CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154(gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling

Application Details

Product Details anti-CD40 Antibody Target Details CD40 Handling References for anti-CD40 Antibody (ABIN2657785) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Antibody Target Details CD40 Application Details References for anti-CD40 Antibody (ABIN2657785) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD40 Antibody (ABIN2657785)

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling Images back to top
Background publications

Bourgeois, Rocha, Tanchot: "A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory." in: Science (New York, N.Y.), Vol. 297, Issue 5589, pp. 2060-3, 2002

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, 1998

Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." in: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, 1994

Images

Product Details anti-CD40 Antibody Target Details CD40 Application Details Handling References for anti-CD40 Antibody (ABIN2657785) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5)  (Alexa Fluor 647) (ABIN2657785) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) antibody (Alexa Fluor 647)