Mouse (Murine) CD40 ELISA Kit

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Antigen
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) ELISA Kits
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Epitope
AA 24-193
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
30
27
5
2
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Options
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Catalog No. ABIN921090
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.865132 ABIN625116 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 3-1500 pg/mL 3 pg/mL Log in to see
9.865132 ABIN424702 Sandwich ELISA Serum, Plasma, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
9.865132 ABIN2708473 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Cell Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
9.865132 ABIN1888499 Sandwich ELISA Plasma, Cell Culture Cells 3-1500 pg/mL 3 pg/mL Log in to see
9.865132 ABIN2113437 Cell Culture Supernatant, Cell Lysate, Serum, Plasma (EDTA - heparin) Log in to see
1 ABIN1572809 Sandwich ELISA Biological Fluids, Plasma, Serum 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN4993029 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.1-1500 pg/mL 2.1 pg/mL Log in to see
1 ABIN829701 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.2-2000 pg/mL 31.2 pg/mL Log in to see
1 ABIN1114189 Sandwich ELISA Serum, Plasma, Biological Fluids 0.781-50 ng/mL 0.781 ng/mL Log in to see
1 ABIN5594504 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1380475 Cell ELISA Log in to see
1 ABIN1380999 Cell ELISA Log in to see
1 ABIN1112576 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN510782 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
1 ABIN2042401 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN5067989 Sandwich ELISA Plasma, Serum Log in to see
1 ABIN2065525 Cell ELISA Log in to see
1 ABIN2683739 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1846881 Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN5068093 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Log in to see

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General

Antigen CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) ELISA Kits
Epitope AA 24-193
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(30), (27), (5), (2), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details CD40 ELISA Kit

Target details Application Details Handling ProductDetails: References for CD40 Kit (ABIN921090) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD40/TNFRSF5
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: V24-R193
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: V24-R193

Target details

Product Details CD40 ELISA Kit Application Details Handling ProductDetails: References for CD40 Kit (ABIN921090) Images back to top
Antigen
Alternative Name CD40 (CD40 ELISA Kit Abstract)
Background

Protein Function: Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. .

Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Synonyms: Tumor necrosis factor receptor superfamily member 5,B-cell surface antigen CD40,Bp50,CD40L receptor,CD40,Cd40,Tnfrsf5,

Full Gene Name: Tumor necrosis factor receptor superfamily member 5

Cellular Localisation: Isoform I: Cell membrane, Single-pass type I membrane protein.
Gene ID 21939
UniProt P27512
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
Pathways NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details CD40 ELISA Kit Target details Handling ProductDetails: References for CD40 Kit (ABIN921090) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated
Protocol mouse CD40 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD40 has been precoated onto 96-well plates. Standards(NSO, V24-R193) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD40 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CD40 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD40 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 95, Standard deviation: 6.46, CV(%): 6.8
  • Sample 2: n=16, Mean(pg/ml): 336, Standard deviation: 17.47, CV(%): 5.2
  • Sample 3: n=16, Mean(pg/ml): 535, Standard deviation: 26.22, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 120, Standard deviation: 9.96, CV(%): 8.3
  • Sample 2: n=24, Mean(pg/ml): 361, Standard deviation: 23.10, CV(%): 6.4
  • Sample 3: n=24, Mean(pg/ml): 541, Standard deviation: 30.30, CV(%): 5.6
Restrictions For Research Use only

Handling

Product Details CD40 ELISA Kit Target details Application Details ProductDetails: References for CD40 Kit (ABIN921090) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for CD40 Kit (ABIN921090)

Product Details CD40 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Lafage-Pochitaloff, Herman, Birg, Galizzi, Simonetti, Mannoni, Banchereau: "Localization of the human CD40 gene to chromosome 20, bands q12-q13.2." in: Leukemia, Vol. 8, Issue 7, pp. 1172-5, 1994

Images

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Supplier Images
ELISA image for CD40 ELISA Kit (CD40 Molecule, TNF Receptor Superfamily Member 5) (ABIN921090) Mouse CD40/TNFRSF5 PicoKine ELISA Kit standard curve