We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Blocking Antibody (Inhibition)||5 Antibodies|
|Blocking Reagent (BR)||3 Antibodies|
|ELISA (Capture)||4 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||175 Antibodies|
|Functional Studies (Func)||30 Antibodies|
|Immunoassay (IA)||7 Antibodies|
|Immunocytochemistry (ICC)||23 Antibodies|
|Immunofluorescence (IF)||18 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||39 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Antigen||CD40 Ligand (CD40LG) Antibodies|
|Conjugate||This CD40 Ligand antibody is conjugated to Alexa Fluor 647 Alternatives|
Blocking Reagent (BR), Flow Cytometry (FACS), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|9 references available|
|Supplier||Log in to see|
Product Details anti-CD40 Ligand AntibodyTarget Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2657641) Images
|Cross-Reactivity||Cynomolgus, Rhesus Monkey|
|Purification||The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.|
Target Details CD40 LigandProduct Details anti-CD40 Ligand Antibody Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2657641) Images back to top
|Alternative Name||CD154 (CD40LG Antibody Abstract)|
|Background||CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling References for anti-CD40 Ligand Antibody (ABIN2657641) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details References for anti-CD40 Ligand Antibody (ABIN2657641) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C|
References for anti-CD40 Ligand Antibody (ABIN2657641)Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Matus-Nicodemos, Vavassori, Castro-Faix et al.: "Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 4, pp. 2164-71, 2011 (PubMed).
Kuchen, Robbins, Sims et al.: "Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5886-96, 2007 (PubMed).
Nordström, Jendholm, Samuelsson et al.: "The IgD-binding domain of the Moraxella IgD-binding protein MID (MID962-1200) activates human B cells in the presence of T cell cytokines." in: Journal of leukocyte biology, Vol. 79, Issue 2, pp. 319-29, 2006 (PubMed).
Najafian, Khoury: "T cell costimulatory pathways: blockade for autoimmunity." in: Expert opinion on biological therapy, Vol. 3, Issue 2, pp. 227-36, 2003 (PubMed).
Stuart, Racke: "Targeting T cell costimulation in autoimmune disease." in: Expert opinion on therapeutic targets, Vol. 6, Issue 3, pp. 275-89, 2002 (PubMed).
Rushworth, Bravery, Hall et al.: "Inhibition of CD40-mediated endothelial cell activation with antisense oligonucleotides." in: Transplantation, Vol. 73, Issue 4, pp. 635-42, 2002 (PubMed).
Brams, Black, Padlan et al.: "A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation." in: International immunopharmacology, Vol. 1, Issue 2, pp. 277-94, 2001 (PubMed).
Berner, Wolf, Hummel et al.: "Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis." in: Annals of the rheumatic diseases, Vol. 59, Issue 3, pp. 190-5, 2000 (PubMed).
Ford, Barnhart, Shone et al.: "Regulation of CD154 (CD40 ligand) mRNA stability during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 7, pp. 4037-44, 1999 (PubMed).
ImagesProduct Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling References for anti-CD40 Ligand Antibody (ABIN2657641) back to top