anti-Human CD40 Ligand antibody for Immunoprecipitation

Recommended CD40 Ligand Antibody (supplied by: Log in to see )

Antigen
CD40 Ligand (CD40LG) Antibodies
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
  • CD40 ligand
  • TNF superfamily member 5
  • CD40LG
  • cd40l
  • Cd40lg
  • LOC100358388
  • CD154
Reactivity
Human
455
234
55
20
20
10
8
8
6
5
4
3
3
3
3
2
2
1
Host
Mouse
275
167
106
51
35
26
1
1
Clonality
Monoclonal
Conjugate
This CD40 Ligand antibody is conjugated to Alexa Fluor 647
73
58
43
14
13
12
12
11
9
8
8
8
8
8
8
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
401
271
170
92
65
51
49
43
33
20
16
13
10
9
7
7
6
5
4
4
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2657641
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1172349 ICC IHC IP WB Rabbit IgG AA 180-261 Log in to see Polyclonal
1 ABIN1858301 ICC IHC IP WB Rabbit IgG AA 48-244 Log in to see Polyclonal
1 ABIN1495080 IP Sepharose Rabbit N-Term Log in to see Polyclonal
1 ABIN1495079 IP Magnetic Particles Rabbit N-Term Log in to see Polyclonal
1 ABIN1980386 ICC IHC IP WB Rabbit IgG AA 1-261 Log in to see Polyclonal
1 ABIN602915 FACS IP Neut WB Mouse IgG1 Log in to see
1 ABIN2223098 FACS IP IHC ELISA WB Mouse IgG1 Log in to see 7H113
1 ABIN2223111 FACS IP Neut WB Mouse IgG1 Log in to see 9G151
1 ABIN2192075 IA FACS IP Neut WB Mouse Log in to see 24-31 2
1 ABIN1690389 IP Sepharose Rabbit Log in to see Polyclonal
1 ABIN1690041 IP Magnetic Particles Rabbit Log in to see Polyclonal

Top referenced anti-CD40 Ligand antibody for Immunoprecipitation

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Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytometry by Time of Flight (CyTOF) 3 Antibodies
ELISA 149 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 232 Antibodies
Functional Studies (Func) 27 Antibodies
Immunoassay (IA) 4 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 50 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies

General

Antigen CD40 Ligand (CD40LG) Antibodies
Reactivity Human
(455), (234), (55), (20), (20), (10), (8), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (1)
Host Mouse
(275), (167), (106), (51), (35), (26), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD40 Ligand antibody is conjugated to Alexa Fluor 647
(73), (58), (43), (14), (13), (12), (12), (11), (9), (8), (8), (8), (8), (8), (8), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(401), (271), (170), (92), (65), (51), (49), (43), (33), (20), (16), (13), (10), (9), (7), (7), (6), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD40 Ligand Antibody

Target Details CD40 Ligand Application Details Handling ProductDetails: References for anti-CD40 Ligand antibody (ABIN2657641) Images
Cross-Reactivity Cynomolgus, Rhesus Monkey
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Clone 24-31
Isotype IgG1 kappa

Target Details CD40 Ligand

Product Details anti-CD40 Ligand Antibody Application Details Handling ProductDetails: References for anti-CD40 Ligand antibody (ABIN2657641) Images back to top
Antigen
Alternative Name CD154 (CD40LG Antibody Abstract)
Background CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways NF-kappaB Signaling, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Handling ProductDetails: References for anti-CD40 Ligand antibody (ABIN2657641) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details ProductDetails: References for anti-CD40 Ligand antibody (ABIN2657641) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

ProductDetails: References for anti-CD40 Ligand antibody (ABIN2657641)

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling Images back to top
Background publications

Matus-Nicodemos, Vavassori, Castro-Faix, Valentin-Acevedo, Singh, Marcelli, Covey: "Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 4, pp. 2164-71, 2011

Kuchen, Robbins, Sims, Sheng, Phillips, Lipsky, Ettinger: "Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5886-96, 2007

Nordström, Jendholm, Samuelsson, Forsgren, Riesbeck: "The IgD-binding domain of the Moraxella IgD-binding protein MID (MID962-1200) activates human B cells in the presence of T cell cytokines." in: Journal of leukocyte biology, Vol. 79, Issue 2, pp. 319-29, 2006

Najafian, Khoury: "T cell costimulatory pathways: blockade for autoimmunity." in: Expert opinion on biological therapy, Vol. 3, Issue 2, pp. 227-36, 2003

Stuart, Racke: "Targeting T cell costimulation in autoimmune disease." in: Expert opinion on therapeutic targets, Vol. 6, Issue 3, pp. 275-89, 2002

Rushworth, Bravery, Hall, Natt, Parsons, Weiler, Häner, Thompson: "Inhibition of CD40-mediated endothelial cell activation with antisense oligonucleotides." in: Transplantation, Vol. 73, Issue 4, pp. 635-42, 2002

Brams, Black, Padlan, Hariharan, Leonard, Chambers-Slater, Noelle, Newman: "A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation." in: International immunopharmacology, Vol. 1, Issue 2, pp. 277-94, 2001

Berner, Wolf, Hummel, Müller, Reuss-Borst: "Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis." in: Annals of the rheumatic diseases, Vol. 59, Issue 3, pp. 190-5, 2000

Ford, Barnhart, Shone, Covey: "Regulation of CD154 (CD40 ligand) mRNA stability during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 7, pp. 4037-44, 1999

Images

Product Details anti-CD40 Ligand Antibody Target Details CD40 Ligand Application Details Handling ProductDetails: References for anti-CD40 Ligand antibody (ABIN2657641) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD40 Ligand antibody (CD40LG)  (Alexa Fluor 647) (ABIN2657641) anti-CD40 Ligand (CD40LG) antibody (Alexa Fluor 647)