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anti-Human EGF antibody for ELISA (Detection)

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Human
276
105
59
15
12
7
4
3
2
1
Host
Mouse
263
122
29
10
7
3
Clonality
Monoclonal
Conjugate
This EGF antibody is un-conjugated
47
24
21
8
8
8
4
4
4
4
4
4
4
4
4
Application
ELISA (Detection), Immunofluorescence (IF), Western Blotting (WB)
271
268
70
48
46
27
27
22
20
14
11
8
6
5
4
3
2
2
2
1
1
Supplier
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Catalog No. ABIN452469
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087304064 ABIN1504136 ELISA (Detection) EIA Biotin Mouse IgG1 Log in to see S-146

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Application / Reactivity Human
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Dot Blot (DB) 1 Antibodies
ELISA 192 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 53 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Neutralization (Neut) 14 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(276), (105), (59), (15), (12), (7), (4), (3), (2), (1)
Host Mouse
(263), (122), (29), (10), (7), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGF antibody is un-conjugated
(47), (24), (21), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application ELISA (Detection), Immunofluorescence (IF), Western Blotting (WB)
(271), (268), (70), (48), (46), (27), (27), (22), (20), (14), (11), (8), (6), (5), (4), (3), (2), (2), (2), (1), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity Reactive with natural and recombinant Human EGF. Does not cross react with other Human cytokines tested such as IL-1beta, IL-8, MCAF, TGF-beta and SAA
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: Urogastrone, Epidermal growth factor, URG, HOMG4, Pro-epidermal growth factor
Purification Affinity Chromatography on Protein G.
Immunogen Purified recombinant human EGF, 6kDa, 53 amino acid residues
Clone S-146
Isotype IgG1

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Gene ID 1950
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes ELISA. Matched Pair: Clone S-134 Cat. No. : DM1012Clone S-146 Cat. No. : ABIN452469
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Reconstitution Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
Concentration 1.0 mg/mL
Buffer 0.01 M PBS, pH 7.2 without preservatives
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN452469) Staining of methanol-fixed Hela cells by Immunocytochemistry using Human EGF Antibody...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN452469) The antibody recognized recombinant EGF on western blot.