anti-Human EGF antibody for Blocking Antibody

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Reactivity
Human
338
164
88
19
14
7
4
2
2
1
1
1
Host
Rabbit
309
152
29
24
6
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
47
27
22
12
10
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
353
289
129
92
54
33
30
28
26
25
19
14
10
7
5
5
4
3
2
2
2
1
1
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Catalog No. ABIN1002255
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN289332 Inhibition ELISA WB Rabbit Log in to see Polyclonal
1 ABIN289421 Inhibition ELISA WB Goat Log in to see Polyclonal

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(338), (164), (88), (19), (14), (7), (4), (2), (2), (1), (1), (1)
Host Rabbit
(309), (152), (29), (24), (6), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(47), (27), (22), (12), (10), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(353), (289), (129), (92), (54), (33), (30), (28), (26), (25), (19), (14), (10), (7), (5), (5), (4), (3), (2), (2), (2), (1), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint hEGF (human EGF)
Isotype IgG

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name Epidermal Growth Factor (EGF Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF (human EGF). Anti-Human EGF specific antibody was purified by affinity chromatography employing immobilized hEGF matrix.
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of hEGF (25.0 pg/µl), a concentration of <0.1 µg/µl of this antibody is required. To detect hEGF by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. To detect hEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Human EGF (PK-AB815-60170B) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Lyophilized
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months