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anti-Human EGF antibody for Western Blotting

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
Epitope
Middle Region
27
15
13
12
10
9
9
8
8
8
7
6
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human
325
142
84
17
13
7
4
2
1
1
1
Host
Rabbit
292
144
30
10
7
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
48
25
22
10
8
8
6
6
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
1
1
Application
Western Blotting (WB)
337
286
110
70
54
32
27
24
20
20
17
14
10
7
6
5
4
2
2
2
2
1
1
1
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Catalog No. ABIN2776844
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1003003 ABIN952036 EIA IHC (p) WB Rabbit Ig AA 689-720, Middle Region Log in to see Polyclonal 3
3.1003003 ABIN1449300 FACS IF IP WB Mouse IgG2b AA 648-1186 Log in to see 6F1 9
3.1003003 ABIN969091 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11 3
3.1003003 ABIN1724732 ICC ELISA WB Mouse IgG1 Log in to see 4-00E-11 2
3.1003003 ABIN446990 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 3
3.1003003 ABIN223519 Neut WB Rabbit IgG Log in to see Polyclonal 1
3.1003003 ABIN452469 ELISA (Detection) IF WB Mouse IgG1 Log in to see S-146
3.1003003 ABIN1107062 EIA IF WB Mouse IgG1 Log in to see 4-00E-011 3
3.1003003 ABIN3044516 ELISA WB Rabbit IgG AA 971-1023 Log in to see Polyclonal
3.1003003 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1003003 ABIN1537657 WB Rabbit Ig AA 940-967, C-Term Log in to see Polyclonal
3.1003003 ABIN498560 IHC (p) WB Rabbit Log in to see Polyclonal
3.1003003 ABIN1872450 IHC WB Rabbit IgG Log in to see Polyclonal
3.1003003 ABIN2801138 WB Rabbit Log in to see Polyclonal
3.1003003 ABIN4307157 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.1003003 ABIN4307158 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.1003003 ABIN2160822 IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
3.1003003 ABIN2160821 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
3.1003003 ABIN2167746 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
3.1003003 ABIN2167745 IF ELISA WB Mouse IgG1 Log in to see 4-00E-011

Top referenced anti-EGF antibody for Western Blotting

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Application / Reactivity Human
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Blocking Peptide (BP) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 205 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope Middle Region
(27), (15), (13), (12), (10), (9), (9), (8), (8), (8), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(325), (142), (84), (17), (13), (7), (4), (2), (1), (1), (1)
Host Rabbit
(292), (144), (30), (10), (7), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(48), (25), (22), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Western Blotting (WB)
(337), (286), (110), (70), (54), (32), (27), (24), (20), (20), (17), (14), (10), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN2776844) Images
Blocking Peptide Blocking peptide for this product available: ABIN976172
Sequence ITIDFLTDKL YWCDAKQSVI EMANLDGSKR RRLTQNDVGH PFAVAVFEDY
Predicted Reactivity Human : 100 %, Chicken : 90 %, Horse : 90 %, Pig : 90 %, Dog : 80 %, Rat : 80 %, Mouse : 70 %
Characteristics This is a rabbit polyclonal antibody against EGF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human EGF

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF Antibody (ABIN2776844) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-
Molecular Weight 6 kDa
Gene ID 1950
NCBI Accession NM_001963, NP_001954
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF Antibody (ABIN2776844) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1207 AA

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF Antibody (ABIN2776844) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EGF Antibody (ABIN2776844)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Teixeira, Ribeiro, Cardoso, Pinto, Lobo, Fraga, Pina, Calais-da-Silva, Medeiros: "Genetic polymorphism in EGF is associated with prostate cancer aggressiveness and progression-free interval in androgen blockade-treated patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 11, pp. 3367-71, 2008

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN2776844) back to top
Supplier Images
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (Middle Region) (ABIN2776844) WB Suggested Anti-EGF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...