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anti-Human EGF antibody for Western Blotting

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Epitope
Middle Region
30
19
15
14
11
10
8
7
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
313
124
76
15
13
7
4
3
2
1
Host
Rabbit
281
140
29
11
7
4
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
48
24
22
10
8
8
6
6
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Western Blotting (WB)
305
274
91
69
46
41
27
23
20
17
17
14
11
8
6
5
4
2
2
2
2
1
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Catalog No. ABIN2776844
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN952036 EIA IHC (p) WB Rabbit Ig AA 689-720, Middle Region Log in to see Polyclonal 3
0.13341247 ABIN1449300 FACS IF IP WB Mouse IgG2b AA 648-1186 Log in to see 6F1 9
0.13341247 ABIN969091 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11 3
0.13341247 ABIN223519 Neut WB Rabbit IgG Log in to see Polyclonal 1
0.13341247 ABIN1724732 ICC ELISA WB Mouse IgG1 Log in to see 4-00E-11 2
0.13341247 ABIN452469 ELISA (Detection) IF WB Mouse IgG1 Log in to see S-146
0.13341247 ABIN1107062 EIA IF WB Mouse IgG1 Log in to see 4-00E-011 3
0.13341247 ABIN3044516 ELISA WB Rabbit IgG AA 971-1023 Log in to see Polyclonal
0.13341247 ABIN498560 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN1537657 WB Rabbit Ig AA 940-967, C-Term Log in to see Polyclonal
0.13341247 ABIN1872450 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2160821 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.13341247 ABIN2160822 IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.13341247 ABIN1872449 ICC WB Mouse Log in to see
0.13341247 ABIN2167745 IF ELISA WB Mouse IgG1 Log in to see 4-00E-011
0.13341247 ABIN2167746 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11
0.13341247 ABIN3201442 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 609-751 Log in to see
0.13341247 ABIN1077992 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 609-751 Log in to see Polyclonal
0.13341247 ABIN1858692 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 971-1023 Log in to see Polyclonal
0.13341247 ABIN933589 ELISA WB Mouse IgG1 Log in to see KT57

Top referenced anti-EGF antibody for Western Blotting

Similar anti-EGF Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 194 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 18 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 72 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 10 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope Middle Region
(30), (19), (15), (14), (11), (10), (8), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(313), (124), (76), (15), (13), (7), (4), (3), (2), (1)
Host Rabbit
(281), (140), (29), (11), (7), (4)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(48), (24), (22), (10), (8), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Western Blotting (WB)
(305), (274), (91), (69), (46), (41), (27), (23), (20), (17), (17), (14), (11), (8), (6), (5), (4), (2), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN2776844) Images
Blocking Peptide Blocking peptide for this product available: ABIN976172
Sequence ITIDFLTDKL YWCDAKQSVI EMANLDGSKR RRLTQNDVGH PFAVAVFEDY
Predicted Reactivity Human : 100 %, Chicken : 90 %, Horse : 90 %, Pig : 90 %, Dog : 80 %, Rat : 80 %, Mouse : 70 %
Characteristics This is a rabbit polyclonal antibody against EGF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human EGF

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF Antibody (ABIN2776844) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-
Molecular Weight 6 kDa
Gene ID 1950
NCBI Accession NM_001963, NP_001954
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF Antibody (ABIN2776844) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1207 AA

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF Antibody (ABIN2776844) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EGF Antibody (ABIN2776844)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Teixeira, Ribeiro, Cardoso, Pinto, Lobo, Fraga, Pina, Calais-da-Silva, Medeiros: "Genetic polymorphism in EGF is associated with prostate cancer aggressiveness and progression-free interval in androgen blockade-treated patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 11, pp. 3367-71, 2008

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF Antibody (ABIN2776844) back to top
Supplier Images
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (Middle Region) (ABIN2776844) WB Suggested Anti-EGF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...