You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Pig (Porcine) EGF antibody for ELISA

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Epitope
AA 45-275
19
15
14
13
11
10
7
7
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Wild boar (Sus scrofa), Pig (Porcine)
277
105
59
15
11
7
3
3
2
1
Host
Rabbit
262
123
29
10
7
3
Clonality
Polyclonal
Conjugate
This EGF antibody is conjugated to Biotin
46
24
21
8
8
8
4
4
4
4
4
4
4
4
4
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
270
268
69
48
46
27
27
22
20
15
11
8
6
5
4
3
2
2
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1173139
$ 457.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012155735 ABIN3201444 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 45-275 Log in to see
0.0012155735 ABIN1173137 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 45-275 Log in to see Polyclonal
0.0012155735 ABIN2599650 ELISA FITC Rabbit IgG AA 45-275 Log in to see Polyclonal
0.0012155735 ABIN2599642 ELISA Biotin Rabbit IgG AA 45-275 Log in to see Polyclonal
0.0012155735 ABIN2599653 ELISA Mouse IgG AA 45-275 Log in to see
0.0012155735 ABIN2599638 ELISA Rabbit IgG AA 45-275 Log in to see Polyclonal

Similar anti-EGF Antibodies

Application / Reactivity Wild boar (Sus scrofa) Pig (Porcine)
ELISA 3 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 3 Antibodies
Immunofluorescence (IF) 1 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 2 Antibodies
Western Blotting (WB) 4 Antibodies 7 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope AA 45-275
(19), (15), (14), (13), (11), (10), (7), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Wild boar (Sus scrofa), Pig (Porcine)
(277), (105), (59), (15), (11), (7), (3), (3), (2), (1)
Host Rabbit
(262), (123), (29), (10), (7), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is conjugated to Biotin
(46), (24), (21), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(270), (268), (69), (48), (46), (27), (27), (22), (20), (15), (11), (8), (6), (5), (4), (3), (2), (2), (2), (2), (1)
Supplier Log in to see

Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Sequence MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
Specificity It has been selected for its ability to recognize EGF in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant EGF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin.
Isotype IgG

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Research Area Growth Factors, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant EGF (Ala45~His275) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (AA 45-275) (Biotin) (ABIN1173139) anti-Epidermal Growth Factor (EGF) (AA 45-275) antibody (Biotin)