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anti-Rat (Rattus) EGF antibody for Western Blotting

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • AI790464
  • CEGF
  • HOMG4
  • URG
Reactivity
Human, Mouse (Murine), Rat (Rattus)
276
104
58
15
12
7
4
3
2
1
Host
Rabbit
262
123
29
10
7
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
47
24
21
8
8
8
4
4
4
4
4
4
4
4
4
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
271
268
70
47
46
27
27
22
20
15
11
8
6
5
4
3
2
2
2
2
1
Supplier
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Catalog No. ABIN498560
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13029787 ABIN1872450 IHC WB Rabbit IgG Log in to see Polyclonal
0.13029787 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13029787 ABIN2428071 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011766474 ABIN1385702 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.0011766474 ABIN465662 IHC Neut ELISA WB Rabbit Log in to see Polyclonal
0.0011766474 ABIN1453236 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011766474 ABIN1414185 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0011766474 ABIN1396308 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011766474 ABIN1819319 Func ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011766474 ABIN1385701 IF (p) IHC (p) WB Mouse IgG Log in to see Polyclonal
0.0011766474 ABIN293787 ELISA WB Sheep IgG Log in to see Polyclonal
0.0011766474 ABIN465685 ELISA WB Biotin Rabbit Log in to see Polyclonal
0.0011766474 ABIN1077994 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 974-1026 Log in to see Polyclonal
0.0011766474 ABIN446990 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.0011766474 ABIN2169419 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011766474 ABIN3201446 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
0.0011766474 ABIN2416059 IF IP ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011766474 ABIN2260977 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011766474 ABIN636690 Inhibition ELISA WB Rabbit Log in to see Polyclonal
0.0011766474 ABIN1002257 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-EGF antibody for Western Blotting

Similar anti-EGF Antibodies

Application / Reactivity Rat (Rattus) Human Mouse (Murine)
Western Blotting (WB) 32 Antibodies 181 Antibodies 73 Antibodies
Radioimmunoassay (RIA) 2 Antibodies 2 Antibodies
Neutralization (Neut) 5 Antibodies 14 Antibodies 8 Antibodies
Immunoprecipitation (IP) 2 Antibodies 10 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies 18 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 53 Antibodies 15 Antibodies 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 7 Antibodies

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(276), (104), (58), (15), (12), (7), (4), (3), (2), (1)
Host Rabbit
(262), (123), (29), (10), (7), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(47), (24), (21), (8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(271), (268), (70), (47), (46), (27), (27), (22), (20), (15), (11), (8), (6), (5), (4), (3), (2), (2), (2), (2), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity This antibody detects endogenous levels of EGF protein. (region surrounding Arg709)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein of 6 kDa originally found in rodents and humans. EGF has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. It binds to the 170 kDa EGF receptor on the surface of cells and mediates intrinsic phos phoryl ation of the receptor on tyrosine residues. EGF has been detected in nearly all body fluids such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF, TGFalpha and vaccinia virus growth factor exhibit 30-40 % amino acid homology. Several additional members of the EGF/TGF family have been described, these include Cripto, Amphiregulin and the heparin-binding EGF-like growth factor, each of which, except Cripto, binds to the EGF receptor.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
Molecular Weight approx. 130 kDa
Gene ID 1950
NCBI Accession NP_001954
UniProt P01133
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN498560) Immunohistochemistry (IHC) analysis of EGF antibody in paraffin-embedded human...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN498560) Western blot analysis of EGF in extracts from NIH-3T3 cells using anti-EGF ant...