anti-Human EGFR antibody for ELISA (Capture)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • LOC5564544
Epitope
AA 25-645
147
135
64
63
59
57
56
54
53
52
48
48
43
42
39
36
33
30
30
29
27
26
25
25
22
19
18
18
18
18
17
16
16
16
15
15
15
15
15
15
14
14
13
11
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2440
850
684
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Goat
1480
859
81
44
8
3
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
104
82
73
65
56
54
54
32
32
28
28
28
28
28
28
28
26
25
24
24
21
21
21
21
21
21
20
18
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
1468
677
615
556
501
272
259
228
123
84
66
56
39
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN4899925
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1982052 ELISA (Capture) FACS IP WB Sheep IgG AA 25-645 Log in to see Polyclonal

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 25-645
(147), (135), (64), (63), (59), (57), (56), (54), (53), (52), (48), (48), (43), (42), (39), (36), (33), (30), (30), (29), (27), (26), (25), (25), (22), (19), (18), (18), (18), (18), (17), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (11), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2440), (850), (684), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Goat
(1480), (859), (81), (44), (8), (3), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(104), (82), (73), (65), (56), (54), (54), (32), (32), (28), (28), (28), (28), (28), (28), (28), (26), (25), (24), (24), (21), (21), (21), (21), (21), (21), (20), (18), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(1468), (677), (615), (556), (501), (272), (259), (228), (123), (84), (66), (56), (39), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
Supplier Log in to see

Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4899925) Images
Specificity Detects human EGFR in ELISAs and Western blots. In sandwich ELISAs, approximately 3 % cross-reactivity with recombinant mouse EGFR is observed and less than 0.1 % cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EGFR, Leu25-Ser645, Accession # CAA25240
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN4899925) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Alternative Names: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene ID 1956
Research Area Receptors, Growth Factors, Hormones, Cancer, Tyrosine Kinases
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN4899925) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 1-15 μg/mL
Immunoprecipitation 1 μg/mL
Immunocytochemistry 1-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN4899925) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EGFR antibody (ABIN4899925)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Burke, Zsengellér, Khankin, Lo, Rajakumar, DuPont, McCurley, Moss, Zhang, Clark, Wang, Seely, Kang, Stillman, Jaffe, Karumanchi: "Soluble fms-like tyrosine kinase 1 promotes angiotensin II sensitivity in preeclampsia." in: The Journal of clinical investigation, Vol. 126, Issue 7, pp. 2561-74, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Nanjo, Yamada, Nishihara, Takeuchi, Sano, Nakagawa, Ishikawa, Zhao, Ebi, Yasumoto, Matsumoto, Yano: "Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors." in: PLoS ONE, Vol. 8, Issue 12, pp. e84700, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sarup, Jin, Turin, Bai, Beryt, Brdlik, Higaki, Jorgensen, Lau, Lindley, Liu, Ni, Rozzelle, Kumari, Watson, Zhang, Shepard: "Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts." in: Molecular cancer therapeutics, Vol. 7, Issue 10, pp. 3223-36, 2008 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Gonzalez, Seurynck-Servoss, Crowley, Brown, Omenn, Hayes, Zangar: "Development and validation of sandwich ELISA microarrays with minimal assay interference." in: Journal of proteome research, Vol. 7, Issue 6, pp. 2406-14, 2008 (Sample species: Human).

Kunii, Davis, Gorenstein, Hatch, Yashiro, Di Bacco, Elbi, Lutterbach: "FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival." in: Cancer research, Vol. 68, Issue 7, pp. 2340-8, 2008 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Murphy, Ghosh, Fulford, Khwaja, Halka, Carter, Turner, Walker: "Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration." in: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, Vol. 31, Issue 5, pp. 516-22, 2006 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN4899925) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 25-645) antibody (ABIN4899925) Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithe...
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 25-645) antibody (ABIN4899925) Detection of Human EGF R/ErbB1 by Western Blot. Western blot shows lysates of HeLa hu...
Immunocytochemistry (ICC) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 25-645) antibody (ABIN4899925) EGF R/ErbB1 in A431 Human Cell Line. EGF R/ErbB1 was detected in immersion fixed A431...
Immunohistochemistry (IHC) image for anti-Epidermal Growth Factor Receptor (EGFR) (AA 25-645) antibody (ABIN4899925) EGF R/ErbB1 in Human Skin. EGF R/ErbB1 was detected in immersion fixed frozen section...