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anti-Human EGFR antibody for ELISA (Capture)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 25-645
148
121
61
58
54
53
52
50
48
48
48
43
36
35
33
30
27
26
25
25
24
23
22
21
19
18
18
17
17
17
16
16
15
15
15
15
15
15
15
14
13
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2241
844
697
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Goat
1383
760
67
43
7
2
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
74
66
59
50
48
26
26
25
24
24
24
23
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
1304
594
557
484
436
272
222
221
108
84
66
55
35
24
19
13
11
8
6
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN4899925
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1982052 ELISA (Capture) FACS IP WB Sheep IgG AA 25-645 Log in to see Polyclonal

Top referenced anti-EGFR antibody for ELISA (Capture)

  • Human Polyclonal EGFR Primary Antibody for ELISA (Capture), FACS - ABIN4899925 (6 Publications): Sarup, Jin, Turin, Bai, Beryt, Brdlik, Higaki, Jorgensen, Lau, Lindley, Liu, Ni, Rozzelle, Kumari, Watson, Zhang, Shepard: Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts. in Molecular cancer therapeutics 2008 (PubMed)

Similar anti-EGFR Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 8 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cell Culture (CC) 1 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 35 Antibodies
Dot Blot (DB) 84 Antibodies
ELISA 544 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 594 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 19 Antibodies
Immunocytochemistry (ICC) 107 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 25-645
(148), (121), (61), (58), (54), (53), (52), (50), (48), (48), (48), (43), (36), (35), (33), (30), (27), (26), (25), (25), (24), (23), (22), (21), (19), (18), (18), (17), (17), (17), (16), (16), (15), (15), (15), (15), (15), (15), (15), (14), (13), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2241), (844), (697), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Goat
(1383), (760), (67), (43), (7), (2), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(97), (74), (66), (59), (50), (48), (26), (26), (25), (24), (24), (24), (23), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(1304), (594), (557), (484), (436), (272), (222), (221), (108), (84), (66), (55), (35), (24), (19), (13), (11), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN4899925) Images
Specificity Detects human EGF R/ErbB1 in ELISAs and Western blots. In sandwich ELISAs, approximately 3 % cross-reactivity with recombinant mouse EGF R is observed and less than 0.1 % cross-reactivity with recombinant human (rh) ErbB2 and rhErbB3 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EGF R/ErbB1, Leu25-Ser645, Accession # CAA25240
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN4899925) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Alternative Names: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGFR, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene ID 1956
Research Area Receptors, Growth Factors, Hormones, Cancer, Tyrosine Kinases
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN4899925) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 1-15 μg/mL
Immunoprecipitation 1 μg/mL
Immunocytochemistry 1-15 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN4899925) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EGFR Antibody (ABIN4899925)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Burke, Zsengellér, Khankin, Lo, Rajakumar, DuPont, McCurley, Moss, Zhang, Clark, Wang, Seely, Kang, Stillman, Jaffe, Karumanchi: "Soluble fms-like tyrosine kinase 1 promotes angiotensin II sensitivity in preeclampsia." in: The Journal of clinical investigation, Vol. 126, Issue 7, pp. 2561-74, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Nanjo, Yamada, Nishihara, Takeuchi, Sano, Nakagawa, Ishikawa, Zhao, Ebi, Yasumoto, Matsumoto, Yano: "Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors." in: PLoS ONE, Vol. 8, Issue 12, pp. e84700, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sarup, Jin, Turin, Bai, Beryt, Brdlik, Higaki, Jorgensen, Lau, Lindley, Liu, Ni, Rozzelle, Kumari, Watson, Zhang, Shepard: "Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts." in: Molecular cancer therapeutics, Vol. 7, Issue 10, pp. 3223-36, 2008 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Gonzalez, Seurynck-Servoss, Crowley, Brown, Omenn, Hayes, Zangar: "Development and validation of sandwich ELISA microarrays with minimal assay interference." in: Journal of proteome research, Vol. 7, Issue 6, pp. 2406-14, 2008 (Sample species: Human).

Kunii, Davis, Gorenstein, Hatch, Yashiro, Di Bacco, Elbi, Lutterbach: "FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival." in: Cancer research, Vol. 68, Issue 7, pp. 2340-8, 2008 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Murphy, Ghosh, Fulford, Khwaja, Halka, Carter, Turner, Walker: "Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration." in: European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, Vol. 31, Issue 5, pp. 516-22, 2006 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN4899925) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithe...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) EGF R/ErbB1 in Human Prostate Cancer Tissue. EGF R/ErbB1 was detected in immersion fi...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 25-645) (ABIN4899925) Detection of Human EGF R/ErbB1 by Western Blot. Western blot shows lysates of HeLa hu...