anti-Human EGFR antibody for Intracellular Flow Cytometry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Reactivity
Human
2444
866
700
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1462
867
92
44
11
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is conjugated to APC
103
80
71
64
58
56
34
34
28
28
28
28
28
28
28
27
27
26
26
25
24
21
21
21
21
21
20
19
18
4
2
2
1
Application
Intracellular Flow Cytometry (ICFC)
1452
702
615
556
511
272
260
230
127
84
66
56
45
24
19
14
12
11
7
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2658305
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2658306 ICFC APC Mouse IgG1 kappa Log in to see AY13 6

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Reactivity Human
(2444), (866), (700), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1462), (867), (92), (44), (11), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is conjugated to APC
(103), (80), (71), (64), (58), (56), (34), (34), (28), (28), (28), (28), (28), (28), (28), (27), (27), (26), (26), (25), (24), (21), (21), (21), (21), (21), (20), (19), (18), (4), (2), (2), (1)
Application Intracellular Flow Cytometry (ICFC)
(1452), (702), (615), (556), (511), (272), (260), (230), (127), (84), (66), (56), (45), (24), (19), (14), (12), (11), (7), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN2658305) Images
Purification The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Clone AY13
Isotype IgG1 kappa

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR antibody (ABIN2658305) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR antibody (ABIN2658305) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR antibody (ABIN2658305) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-EGFR antibody (ABIN2658305)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Dong, Wodziak, Lowe: "Epidermal growth factor receptor (EGFR) signaling requires a specific endoplasmic reticulum thioredoxin for the post-translational control of receptor presentation to the cell surface." in: The Journal of biological chemistry, Vol. 290, Issue 13, pp. 8016-27, 2015

Pasternack, Domogauer, Khullar, Akudugu, Howell: "The advantage of antibody cocktails for targeted alpha therapy depends on specific activity." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 55, Issue 12, pp. 2012-9, 2014

da Cunha Santos, Shepherd, Tsao: "EGFR mutations and lung cancer." in: Annual review of pathology, Vol. 6, pp. 49-69, 2011

Pao, Chmielecki: "Rational, biologically based treatment of EGFR-mutant non-small-cell lung cancer." in: Nature reviews. Cancer, Vol. 10, Issue 11, pp. 760-74, 2010

Gusterson, Hunter: "Should we be surprised at the paucity of response to EGFR inhibitors?" in: The Lancet. Oncology, Vol. 10, Issue 5, pp. 522-7, 2009

Mano, Humblet: "Drug Insight: panitumumab, a human EGFR-targeted monoclonal antibody with promising clinical activity in colorectal cancer." in: Nature clinical practice. Oncology, Vol. 5, Issue 7, pp. 415-25, 2008

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR antibody (ABIN2658305) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Epidermal Growth Factor Receptor (EGFR) antibody (APC) (ABIN2658305) anti-Epidermal Growth Factor Receptor (EGFR) antibody (APC)