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anti-Human EGFR antibody for Intracellular Flow Cytometry

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Reactivity
Human
2243
844
698
30
24
18
18
15
15
5
4
2
2
2
1
1
Host
Mouse
1385
759
67
43
8
2
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is conjugated to APC
97
74
66
59
50
48
26
26
25
24
24
23
23
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Intracellular Flow Cytometry (ICFC)
1307
595
557
485
439
272
222
222
109
84
66
55
35
24
19
13
11
8
6
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2658305
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2658306 ICFC APC Mouse IgG1 kappa Log in to see AY13 6

Top referenced anti-EGFR antibody for Intracellular Flow Cytometry

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Application / Reactivity Human
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Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 8 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cell Culture (CC) 1 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 35 Antibodies
Dot Blot (DB) 84 Antibodies
ELISA 544 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 594 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 19 Antibodies
Immunocytochemistry (ICC) 107 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Reactivity Human
(2243), (844), (698), (30), (24), (18), (18), (15), (15), (5), (4), (2), (2), (2), (1), (1)
Host Mouse
(1385), (759), (67), (43), (8), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is conjugated to APC
(97), (74), (66), (59), (50), (48), (26), (26), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Intracellular Flow Cytometry (ICFC)
(1307), (595), (557), (485), (439), (272), (222), (222), (109), (84), (66), (55), (35), (24), (19), (13), (11), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN2658305) Images
Purification The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Clone AY13
Isotype IgG1 kappa

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN2658305) Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN2658305) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN2658305) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-EGFR Antibody (ABIN2658305)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Dong, Wodziak, Lowe: "Epidermal growth factor receptor (EGFR) signaling requires a specific endoplasmic reticulum thioredoxin for the post-translational control of receptor presentation to the cell surface." in: The Journal of biological chemistry, Vol. 290, Issue 13, pp. 8016-27, 2015

Pasternack, Domogauer, Khullar, Akudugu, Howell: "The advantage of antibody cocktails for targeted alpha therapy depends on specific activity." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 55, Issue 12, pp. 2012-9, 2014

da Cunha Santos, Shepherd, Tsao: "EGFR mutations and lung cancer." in: Annual review of pathology, Vol. 6, pp. 49-69, 2011

Pao, Chmielecki: "Rational, biologically based treatment of EGFR-mutant non-small-cell lung cancer." in: Nature reviews. Cancer, Vol. 10, Issue 11, pp. 760-74, 2010

Gusterson, Hunter: "Should we be surprised at the paucity of response to EGFR inhibitors?" in: The Lancet. Oncology, Vol. 10, Issue 5, pp. 522-7, 2009

Mano, Humblet: "Drug Insight: panitumumab, a human EGFR-targeted monoclonal antibody with promising clinical activity in colorectal cancer." in: Nature clinical practice. Oncology, Vol. 5, Issue 7, pp. 415-25, 2008

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN2658305) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EGFR antibody (Epidermal Growth Factor Receptor)  (APC) (ABIN2658305) anti-Epidermal Growth Factor Receptor (EGFR) antibody (APC)