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anti-Human EGFR antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
AA 35-65, N-Term
146
140
58
57
53
51
50
48
46
45
44
44
35
33
31
30
30
25
24
23
23
23
23
22
22
19
18
17
16
14
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
9
8
8
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2159
749
611
23
14
14
13
10
8
7
4
2
1
1
1
1
1
Host
Rabbit
1377
692
63
45
4
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
93
74
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1320
568
511
448
421
250
232
184
86
76
71
54
25
11
11
9
8
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN359820
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN359838 EIA IHC (p) WB Rabbit Ig Ser1070 Log in to see Polyclonal 3
0.34288716 ABIN498778 IHC (p) Rabbit pTyr1092 Log in to see Polyclonal
0.34288716 ABIN498780 IHC (p) Rabbit pTyr1197 Log in to see Polyclonal
0.34288716 ABIN498781 IHC (p) Rabbit pTyr869 Log in to see Polyclonal
0.34288716 ABIN498782 IHC (p) Rabbit pTyr1110 Log in to see Polyclonal
0.34288716 ABIN214559 IHC (p) ELISA WB Rabbit AA 1189-1199 Log in to see Polyclonal
0.34288716 ABIN498727 IHC (p) WB Rabbit pTyr1197 Log in to see Polyclonal
0.34288716 ABIN498779 IHC (p) Rabbit pTyr1172 Log in to see Polyclonal
0.34288716 ABIN457474 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
0.34288716 ABIN213615 IHC (p) Rabbit C-Term Log in to see Polyclonal
0.34288716 ABIN364308 ICC IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal
0.34288716 ABIN213614 IHC (p) Rabbit C-Term Log in to see Polyclonal
0.34288716 ABIN218581 IHC (p) IP ELISA WB Rabbit AA 1166-1180 Log in to see Polyclonal
0.34288716 ABIN498870 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN498871 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN498873 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN498869 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN498872 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN615211 IF IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal
0.34288716 ABIN2649159 IHC (p) Mouse Log in to see

Top referenced anti-EGFR antibody for Immunohistochemistry (Paraffin-embedded Sections)

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Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 86 Antibodies
ELISA 557 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 511 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
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Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 190 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 35-65, N-Term
(146), (140), (58), (57), (53), (51), (50), (48), (46), (45), (44), (44), (35), (33), (31), (30), (30), (25), (24), (23), (23), (23), (23), (22), (22), (19), (18), (17), (16), (14), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2159), (749), (611), (23), (14), (14), (13), (10), (8), (7), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1377), (692), (63), (45), (4), (2)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(93), (74), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1320), (568), (511), (448), (421), (250), (232), (184), (86), (76), (71), (54), (25), (11), (11), (9), (8), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN359820) Images
Specificity This antibody recognozes Human EGFR
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen KLH conjugated synthetic peptide between 35~65 amino acids from the N-terminal region of Human ErbB1
Isotype Ig

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN359820) Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956, 9606
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN359820) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN359820) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-EGFR Antibody (ABIN359820)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Zanardi, Yei, Lichtenstein, Tollefson, Wold: "Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor." in: Journal of virology, Vol. 77, Issue 21, pp. 11685-96, 2003

Krug, Grossmann, Schuster, Freudinger, Mildenberger, Govindan, Gekle: "Aldosterone stimulates epidermal growth factor receptor expression." in: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 43060-6, 2003

Huang, Jiang, Sorkin: "Tyrosine phosphorylation of the beta2 subunit of clathrin adaptor complex AP-2 reveals the role of a di-leucine motif in the epidermal growth factor receptor trafficking." in: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 43411-7, 2003

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN359820) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 35-65) (ABIN359820) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 35-65) (ABIN359820) Western blot analysis of anti-ErbB1 Pab in ZR-75-1 cell lysate. ErbB1 (arrow) was det...