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anti-Human EGFR antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 35-65, N-Term
145
138
61
58
56
54
53
50
48
48
48
44
36
36
33
31
30
27
26
25
25
25
23
22
21
19
19
17
17
16
16
15
15
15
15
15
15
14
14
13
13
12
10
10
10
10
10
9
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2292
781
641
23
16
14
13
10
7
4
3
2
1
1
1
Host
Rabbit
1437
751
69
45
8
2
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
96
76
66
59
48
46
25
24
23
23
23
22
22
21
21
21
21
21
21
21
21
21
21
21
21
20
20
19
18
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1322
586
569
467
447
272
236
229
115
87
73
54
35
25
12
11
8
6
5
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN359820
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.081278 ABIN359838 EIA IHC (p) WB Rabbit Ig Ser1070 Log in to see Polyclonal 3
12.081278 ABIN152027 ICC IF IHC IHC (p) IP WB Rabbit AA 1100-1150 Log in to see Polyclonal 4
12.081278 ABIN152028 ICC IF IHC IHC (p) IP PLA WB Rabbit C-Term Log in to see Polyclonal 1
12.081278 ABIN265742 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 29-1 2
12.081278 ABIN4307205 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
12.081278 ABIN498727 IHC (p) WB Rabbit pTyr1197 Log in to see Polyclonal
12.081278 ABIN498781 IHC (p) Rabbit pTyr869 Log in to see Polyclonal
12.081278 ABIN498778 IHC (p) Rabbit pTyr1092 Log in to see Polyclonal
12.081278 ABIN498779 IHC (p) Rabbit pTyr1172 Log in to see Polyclonal
12.081278 ABIN498782 IHC (p) Rabbit pTyr1110 Log in to see Polyclonal
12.081278 ABIN498780 IHC (p) Rabbit pTyr1197 Log in to see Polyclonal
12.081278 ABIN214559 IHC (p) ELISA WB Rabbit AA 1189-1199 Log in to see Polyclonal
12.081278 ABIN213615 IHC (p) Rabbit C-Term Log in to see Polyclonal
12.081278 ABIN258577 FACS ICC IF IHC IHC (p) IP Mouse IgG2a Log in to see 528
12.081278 ABIN315685 IHC (p) IHC ELISA WB Rabbit pTyr1197 Log in to see Polyclonal
12.081278 ABIN725720 IF (p) IHC (p) Rabbit IgG AA 975-1015 Log in to see Polyclonal
12.081278 ABIN457474 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
12.081278 ABIN4307186 IHC IHC (p) WB Rabbit pTyr1092 Log in to see Polyclonal
12.081278 ABIN4307221 FACS IF IHC (p) Mouse IgG2a kappa Log in to see GFR450
12.081278 ABIN213614 IHC (p) Rabbit C-Term Log in to see Polyclonal

Top referenced anti-EGFR antibody for Immunohistochemistry (Paraffin-embedded Sections)

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General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 35-65, N-Term
(145), (138), (61), (58), (56), (54), (53), (50), (48), (48), (48), (44), (36), (36), (33), (31), (30), (27), (26), (25), (25), (25), (23), (22), (21), (19), (19), (17), (17), (16), (16), (15), (15), (15), (15), (15), (15), (14), (14), (13), (13), (12), (10), (10), (10), (10), (10), (9), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2292), (781), (641), (23), (16), (14), (13), (10), (7), (4), (3), (2), (1), (1), (1)
Host Rabbit
(1437), (751), (69), (45), (8), (2), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(96), (76), (66), (59), (48), (46), (25), (24), (23), (23), (23), (22), (22), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (21), (20), (20), (19), (18), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1322), (586), (569), (467), (447), (272), (236), (229), (115), (87), (73), (54), (35), (25), (12), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN359820) Images
Specificity This antibody recognozes Human EGFR
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen KLH conjugated synthetic peptide between 35~65 amino acids from the N-terminal region of Human ErbB1
Isotype Ig

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN359820) Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956, 9606
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN359820) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN359820) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-EGFR Antibody (ABIN359820)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Background publications

Zanardi, Yei, Lichtenstein, Tollefson, Wold: "Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor." in: Journal of virology, Vol. 77, Issue 21, pp. 11685-96, 2003

Krug, Grossmann, Schuster, Freudinger, Mildenberger, Govindan, Gekle: "Aldosterone stimulates epidermal growth factor receptor expression." in: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 43060-6, 2003

Huang, Jiang, Sorkin: "Tyrosine phosphorylation of the beta2 subunit of clathrin adaptor complex AP-2 reveals the role of a di-leucine motif in the epidermal growth factor receptor trafficking." in: The Journal of biological chemistry, Vol. 278, Issue 44, pp. 43411-7, 2003

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN359820) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 35-65) (ABIN359820) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 35-65) (ABIN359820) Western blot analysis of anti-ErbB1 Pab in ZR-75-1 cell lysate. ErbB1 (arrow) was det...