anti-Human EGFR antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
AA 1100-1150
148
122
62
59
57
53
53
51
48
48
48
43
38
36
33
30
29
27
26
25
25
25
23
22
21
19
19
18
18
17
16
16
16
16
15
15
15
15
15
15
14
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2334
850
699
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Rabbit
1434
798
67
44
9
3
2
1
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
75
66
60
51
49
27
27
26
25
25
24
24
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1362
633
572
528
441
272
244
229
110
84
67
56
35
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN152027
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.400779 ABIN152028 ICC IF IHC IHC (p) IP PLA WB Rabbit C-Term Log in to see Polyclonal 1
13.400779 ABIN265742 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 29-1 2
13.400779 ABIN4307205 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
13.400779 ABIN725720 IF (p) IHC (p) Rabbit IgG AA 975-1015 Log in to see Polyclonal
13.400779 ABIN498727 IHC (p) WB Rabbit pTyr1197 Log in to see Polyclonal
13.400779 ABIN498779 IHC (p) Rabbit pTyr1172 Log in to see Polyclonal
13.400779 ABIN498781 IHC (p) Rabbit pTyr869 Log in to see Polyclonal
13.400779 ABIN498780 IHC (p) Rabbit pTyr1197 Log in to see Polyclonal
13.400779 ABIN498782 IHC (p) Rabbit pTyr1110 Log in to see Polyclonal
13.400779 ABIN498778 IHC (p) Rabbit pTyr1092 Log in to see Polyclonal
13.400779 ABIN457474 ICC IF IHC (p) IP ELISA WB Sheep IgG Internal Region Log in to see Polyclonal
13.400779 ABIN214559 IHC (p) ELISA WB Rabbit AA 1189-1199 Log in to see Polyclonal
13.400779 ABIN4307186 IHC IHC (p) WB Rabbit pTyr1092 Log in to see Polyclonal
13.400779 ABIN4307221 FACS IF IHC (p) Mouse IgG2a kappa Log in to see GFR450
13.400779 ABIN2649159 IHC (p) Mouse IgG1 AA 668-1210 Log in to see 11
13.400779 ABIN213615 IHC (p) Rabbit C-Term Log in to see Polyclonal
13.400779 ABIN315685 IHC (p) IHC ELISA WB Rabbit pTyr1197 Log in to see Polyclonal
13.400779 ABIN258577 FACS ICC IF IHC IHC (p) IP Mouse IgG2a Log in to see 528
13.400779 ABIN364308 ICC IHC (p) IP WB Sheep Internal Region Log in to see Polyclonal
13.400779 ABIN4307285 ICC IF IHC IHC (p) IP WB Mouse IgG2b C-Term Log in to see 133

Top referenced anti-EGFR antibody for Immunohistochemistry (Paraffin-embedded Sections)

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General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope AA 1100-1150
(148), (122), (62), (59), (57), (53), (53), (51), (48), (48), (48), (43), (38), (36), (33), (30), (29), (27), (26), (25), (25), (25), (23), (22), (21), (19), (19), (18), (18), (17), (16), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2334), (850), (699), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1434), (798), (67), (44), (9), (3), (2), (1), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(97), (75), (66), (60), (51), (49), (27), (27), (26), (25), (25), (24), (24), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1362), (633), (572), (528), (441), (272), (244), (229), (110), (84), (67), (56), (35), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN152027) Images
Specificity This antibody is specific for human EGFR protein.
Purification Immunogen affinity purified
Immunogen A synthetic peptide which maps to a region between residues 1100 and 1150 of human Epidermal Growth Factor Receptor using the numbering given in Swiss-Prot entry P00533 (GeneID 1956).

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN152027) Images back to top
Antigen
Alternative Name EGF R/ErbB1 (EGFR Antibody Abstract)
Background Gene Symbol: EGFR
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling ProductDetails: References for anti-EGFR antibody (ABIN152027) Images back to top
Application Notes Western Blot 1:10000-1:50000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:250 - 1:2000, Immunoprecipitation 1 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:10-1:500Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:50,000 Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details ProductDetails: References for anti-EGFR antibody (ABIN152027) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-EGFR antibody (ABIN152027)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Atapattu, Saha, Chheang, Eissman, Xu, Vail, Hii, Llerena, Liu, Horvay, Abud, Kusebauch, Moritz, Ding, Cao, Rafii, Ernst, Scott, Nikolov, Lackmann, Janes: "An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth." in: The Journal of experimental medicine, Vol. 213, Issue 9, pp. 1741-57, 2016 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Liu, Leng, Gunasekaran, Pentony, Shen, Howard, Stoops, Manchulenko, Razinkov, Liu, Fanslow, Hu, Sun, Hasegawa, Clark, Foltz, Yan: "A Novel Antibody Engineering Strategy for Making Monovalent Bispecific Heterodimeric IgG Antibodies by Electrostatic Steering Mechanism." in: The Journal of biological chemistry, 2015

Guo, Stark: "FER tyrosine kinase (FER) overexpression mediates resistance to quinacrine through EGF-dependent activation of NF-kappaB." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 19, pp. 7968-73, 2011

Zhou, Yang: "The von Hippel-Lindau tumor suppressor protein promotes c-Cbl-independent poly-ubiquitylation and degradation of the activated EGFR." in: PLoS ONE, Vol. 6, Issue 9, pp. e23936, 2011

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling ProductDetails: References for anti-EGFR antibody (ABIN152027) back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1100-1150) (ABIN152027) Detection of Human EGFR by Western Blot and Immunoprecipitation. Samples: Whole cell ...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1100-1150) (ABIN152027) Western Blot: EGF R/ErbB1 Antibody [ABIN152027] - Whole cell lysate (50 ug) from HeLa...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (AA 1100-1150) (ABIN152027) Immunohistochemistry-Paraffin: EGF R/ErbB1 Antibody [ABIN152027] - Human placenta. An...