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anti-Human EGFR antibody for Western Blotting

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Reactivity
Human
2147
741
607
21
14
14
12
10
7
7
4
2
1
1
1
Host
Rabbit
1373
687
60
44
4
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
93
73
64
55
45
43
25
24
23
21
21
21
21
21
21
21
20
19
19
19
19
19
19
19
19
19
19
2
2
2
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
1311
564
505
442
414
242
232
180
86
74
67
55
25
11
11
9
8
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN98862
$ 369.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN126767 EIA IF IP WB Mouse IgG1 pSer1071 Log in to see 1H9 1
0.13398378 ABIN1687938 IHC (p) WB Rabbit IgG C-Term Log in to see SP84 1
0.13398378 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
0.13398378 ABIN1532007 ELISA WB Rabbit IgG pSer1026 Log in to see Polyclonal 1
0.13398378 ABIN1532008 ELISA WB Rabbit IgG pSer1071 Log in to see Polyclonal 1
0.13398378 ABIN391933 WB Rabbit Ig AA 956-985, Tyr978 Log in to see Polyclonal 3
0.13398378 ABIN651628 FACS IHC (p) WB Rabbit Ig AA 1004-1033, C-Term Log in to see Polyclonal 1
0.13398378 ABIN387815 WB Mouse IgG1 kappa Log in to see 51CT78-40-5 6
0.13398378 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
0.13398378 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
0.13398378 ABIN1533044 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13398378 ABIN1533045 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13398378 ABIN1532602 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13398378 ABIN1532852 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13398378 ABIN1533046 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13398378 ABIN359828 EIA WB Rabbit Ig Tyr987 Log in to see Polyclonal 5
0.13398378 ABIN359820 EIA IHC (p) WB Rabbit Ig AA 35-65, N-Term Log in to see Polyclonal 3
0.13398378 ABIN359838 EIA IHC (p) WB Rabbit Ig Ser1070 Log in to see Polyclonal 3
0.13398378 ABIN391943 IHC (p) WB Rabbit Ig AA 1048-1077, Ser1070 Log in to see Polyclonal 5
0.13398378 ABIN1724889 FACS ELISA WB Mouse IgG2b AA 693-893 Log in to see 5E10D3 2

Top referenced anti-EGFR antibody for Western Blotting

Similar anti-EGFR Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Reagent (BR) 9 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 86 Antibodies
ELISA 557 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 55 Antibodies
Flow Cytometry (FACS) 510 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 25 Antibodies
Immunocytochemistry (ICC) 74 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 189 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Reactivity Human
(2147), (741), (607), (21), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Rabbit
(1373), (687), (60), (44), (4), (2)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(93), (73), (64), (55), (45), (43), (25), (24), (23), (21), (21), (21), (21), (21), (21), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (2), (2), (2), (1)
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(1311), (564), (505), (442), (414), (242), (232), (180), (86), (74), (67), (55), (25), (11), (11), (9), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Characteristics Concentration Definition: by Refractometry
Immunogen This whole rabbit serum was prepared by repeated immunizations with a peptide synthesized using conventional technology. The sequence of the epitope maps to a region near the carboxy terminus which is identical in human, mouse and rat EGFR.

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial to maintaining a normal balance in cell growth and development.  Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors.  For example, the epidermal growth factor receptor (EGFR) is mutated and/or over-expressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and ErbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis. EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4/neu. EGFR can heterodimerize with each of the members of this family.
Synonyms: Receptor tyrosine-protein kinase erbB-1 antibody, c-erbB-1 antibody
Gene ID 1956, 29725609
UniProt P00533
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Anti-EGFR antibody is specifically designed for ELISA, immunoblotting and immunoprecipitation.  Reactivity in other assays is likely, but has not been determined.  Recognition of EGFR is independent of the phosphorylation status at tyrosine 1173.  No reaction is observed against ErbB-2, ErbB-3 or ErbB-4.   A431 cells, keratinocytes in normal epidermis, or placenta are typically used as positive control sources.  The antigen is typically localized in the cell membrane.   For western blotting, good results are also achieved on PVDF membranes blocked with 5% lowfat milk diluted in TTBS for 1 hour at room temperature.  Also, dilute the primary antibody and secondary in 5% lowfat milk in TTBS.   Anti-EGFR can be diluted up to 1:10,000 for immunoblot depending on the cell line and the amount of EGFR in a particular lysate.  For immunoprecipitation, use approximately 10 µl of the antibody.  The immunoprecipitation mix should contain the antibody, 25 µl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein).  This mixture should be rotated overnight at 4°C and then washed 3 times with lysis buffer (used to prepare the lysate).  The resulting bead complex is dissolved in 20-30 µl of 3X SDS-PAGE sample buffer and approximately 15 µl is loaded per lane on an 8% polyacrylamide gel.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Concentration 85 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN98862) Combined immunoprecipitation and western blot using anti-EGFR antibody. Lysates were ...
 image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN98862) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 2)
 image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (ABIN98862) anti-Epidermal Growth Factor Receptor (EGFR) antibody (Image 3)
'Independent Validation' Badge
Lot Number 19538
Method validated Western Blotting
Positive Control A549 cells
Negative Control MCF-7 cells
Notes A strong specific band was observed in the positive control at the expected size (~175 kDa) that is not observed in the negative control.
Primary Antibody
  • Antigen: Epidermal Growth Factor Receptor (EGFR)
  • Catalog number: ABIN98862
  • Supplier: Log in to see
  • Lot number: 19538
  • Dilution: 1:1,000
Secondary Antibody
  • Antibody: IRDye 680LT Goat Anti-Rabbit
  • Catalogue number: Log in to see
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  • Lot number: C30725-01
  • Dilution: 1:10,000
Controls - Lysates were prepared by ADS Biosystems following standard protocols and quality controlled for protein integrity on a regular basis
Protocol
  • Lysates were mixed with NuPAGE® LDS Sample Buffer (Life Technologies NP0007) and NuPAGE® Sample Reducing Agent (Life Technologies NP0004) and denatured for 5 minutes at 90ºC.
  • 40 μg of each lysate was electrophoresed on a Bolt 4-12% Bis-Tris Gel (Life Technologies BG04120BOX) and run in Bolt MOPS SDS Running Buffer (Life Technologies B0001) at 160 volts for 1 hour.
  • Odyssey Western Protein Standard (LI-COR #928-40000) was run as a molecular weight standard.
  • PVDF membrane was activated with methanol.
  • Protein samples were transferred to activated PVDF membrane in a wet Bolt Transfer Apparatus (Life Technologies B1000) at room temperature for 1 hour at 20 volts (started at 230mA, ended at 110mA).
  • The membrane was blocked in x LI-COR Odyssey WB block solution for 1 hour at room temperature.
  • The membrane was incubated with the primary antibody diluted 1:1000 in x LI-COR Odyssey WB block solution incubated 2 hours at room temperature.
  • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
  • The membrane was incubated with IRDye® 800CW Goat anti-Mouse Secondary Antibody (Red) and IRDye 680LT Goat Anti-Rabbit Secondary Antibody (Green) from LI-COR (#827-11081, Lot #C30725-01), both 1:10,000 dilutions. Incubation was performed at room temperature for 45 minutes.
  • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
  • Proteins were detected using Odyssey machine scanning with green channel for loading control and red channel for potential LPL band.
Experimental Notes - No experimental challenges noted.
Validation Images
Western Blotting validation image for anti-Epidermal Growth Factor Receptor (EGFR) antibody (ABIN98862) Figure 1: Scanned image of EGFR (Green) and loading control alpha-tubulin (Red) Weste...