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anti-Mouse (Murine) EGFR antibody for Immunohistochemistry (Frozen Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
pTyr869
145
139
59
57
54
52
51
49
47
46
45
44
36
34
32
31
30
26
25
24
24
24
23
22
22
19
18
17
17
16
16
14
14
14
14
14
14
14
13
13
13
11
10
10
10
9
9
9
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2196
763
625
23
16
14
13
10
8
5
4
2
1
1
1
1
1
Host
Mouse
1398
697
69
45
7
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
94
74
64
57
46
44
44
25
23
21
21
21
21
21
21
21
20
20
19
19
19
19
19
19
19
19
16
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1332
577
527
447
427
252
244
184
87
74
72
54
25
11
11
8
6
6
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126761
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008879846 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
0.0008879846 ABIN126759 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr869 Log in to see 12A3
0.0008879846 ABIN126776 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr1173 Log in to see 9H2
0.0008879846 ABIN153570 FACS IHC (fro) IHC (p) IP IHC Rat IgG2a Log in to see ICR10
0.0008879846 ABIN1867707 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 319-484 Log in to see Polyclonal
0.0008879846 ABIN4269287 FACS IHC (fro) IHC (p) WB Alexa Fluor 488 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269290 FACS IHC (fro) IHC (p) WB Allophycocyanin Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269286 FACS IHC (fro) IHC (p) WB Alexa Fluor 405 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269289 FACS IHC (fro) IHC (p) WB Alexa Fluor 700 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269291 FACS IHC (fro) IHC (p) WB Biotin Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269299 FACS IHC (fro) IHC (p) WB PE Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269302 FACS IHC (fro) IHC (p) WB DyLight 405 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269303 FACS IHC (fro) IHC (p) WB DyLight 405LS Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269288 FACS IHC (fro) IHC (p) WB Alexa Fluor 647 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269293 FACS IHC (fro) IHC (p) WB FITC Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269298 FACS IHC (fro) IHC (p) WB PerCP Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269292 FACS IHC (fro) IHC (p) WB DyLight 650 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269294 FACS IHC (fro) IHC (p) WB DyLight 680 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269300 FACS IHC (fro) IHC (p) WB DyLight 550 Rat IgG2a Log in to see ICR10
0.0008879846 ABIN4269297 FACS IHC (fro) IHC (p) WB DyLight 755 Rat IgG2a Log in to see ICR10

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 136 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 67 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 77 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 204 Antibodies
Immunohistochemistry (IHC) 185 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 131 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 482 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 8 Antibodies
Blocking Reagent (BR) 6 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr869
(145), (139), (59), (57), (54), (52), (51), (49), (47), (46), (45), (44), (36), (34), (32), (31), (30), (26), (25), (24), (24), (24), (23), (22), (22), (19), (18), (17), (17), (16), (16), (14), (14), (14), (14), (14), (14), (14), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2196), (763), (625), (23), (16), (14), (13), (10), (8), (5), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(1398), (697), (69), (45), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(94), (74), (64), (57), (46), (44), (44), (25), (23), (21), (21), (21), (21), (21), (21), (21), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (16), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1332), (577), (527), (447), (427), (252), (244), (184), (87), (74), (72), (54), (25), (11), (11), (8), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity Clone 12A3 specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Antibody AM00031PU-N does not crossreact with the highly homologues pTyr 877 of activated erbB2.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLHEpitope: Phosphotyrosine 869AA Sequence: E K E pY H A E
Clone 12A3
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes ELISA: Use at 0.1 μg/mL (direct ELISA). Immunocytochemistry: Use at 1-10 μg/mL. Immunohistochemistry on Frozen and Paraffin Embedded Material. Immunoprecipitation: Use at 1-10 μg per 10^6 vanadate-treated A431 cells. Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer (cat#AS00002BU-N). Included Positive Control: Cell lysate delivered with this product (See Protocol below! ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive control cell lysate provided: HepG2 EGF Treated - recommended for immunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withEGF. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoidrepeated freezing and thawing. Applications: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) forHRPO/ECL detection of the target proteins.
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Reconstitution Restore in 1 mL distilled water for 15 min at RT.
Buffer PBS containing 0.09 % Sodium Azide / PEG and Sucrose.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at-80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3months. Avoid repeated freeze / thaw cycles!
Expiry Date 12 months

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (pTyr869) (ABIN126761) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr869) antibody