anti-Mouse (Murine) EGFR antibody for Immunohistochemistry (Frozen Sections)

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
pTyr1173
147
138
64
64
60
58
57
54
54
53
49
49
43
37
36
33
30
30
29
27
26
25
25
23
22
19
19
18
18
16
16
16
16
15
15
15
15
15
14
14
13
11
9
9
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
2416
864
699
28
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Mouse
1458
856
81
44
9
3
2
1
1
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
102
80
71
63
56
54
32
32
28
28
28
28
28
28
27
26
26
25
25
24
24
21
21
21
21
21
20
19
18
2
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1444
678
615
554
508
272
259
228
124
84
65
55
40
24
19
14
12
11
7
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN126779
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN153570 FACS IHC (fro) IHC (p) IP IHC Rat IgG2a Log in to see ICR10 1
1 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN126776 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr1173 Log in to see 9H2
1 ABIN126759 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN4269286 FACS IHC (fro) IHC (p) WB Alexa Fluor 405 Rat IgG2a Log in to see ICR10
1 ABIN4269287 FACS IHC (fro) IHC (p) WB Alexa Fluor 488 Rat IgG2a Log in to see ICR10
1 ABIN4269288 FACS IHC (fro) IHC (p) WB Alexa Fluor 647 Rat IgG2a Log in to see ICR10
1 ABIN4269289 FACS IHC (fro) IHC (p) WB Alexa Fluor 700 Rat IgG2a Log in to see ICR10
1 ABIN4269290 FACS IHC (fro) IHC (p) WB Allophycocyanin Rat IgG2a Log in to see ICR10
1 ABIN4269291 FACS IHC (fro) IHC (p) WB Biotin Rat IgG2a Log in to see ICR10
1 ABIN4269292 FACS IHC (fro) IHC (p) WB DyLight 650 Rat IgG2a Log in to see ICR10
1 ABIN4269293 FACS IHC (fro) IHC (p) WB FITC Rat IgG2a Log in to see ICR10
1 ABIN4269294 FACS IHC (fro) IHC (p) WB DyLight 680 Rat IgG2a Log in to see ICR10
1 ABIN4269295 FACS IHC (fro) IHC (p) WB DyLight 488 Rat IgG2a Log in to see ICR10
1 ABIN4269297 FACS IHC (fro) IHC (p) WB DyLight 755 Rat IgG2a Log in to see ICR10
1 ABIN4269298 FACS IHC (fro) IHC (p) WB PerCP Rat IgG2a Log in to see ICR10
1 ABIN4269299 FACS IHC (fro) IHC (p) WB PE Rat IgG2a Log in to see ICR10
1 ABIN4269300 FACS IHC (fro) IHC (p) WB DyLight 550 Rat IgG2a Log in to see ICR10
1 ABIN4269301 FACS IHC (fro) IHC (p) WB DyLight 350 Rat IgG2a Log in to see ICR10
1 ABIN4269302 FACS IHC (fro) IHC (p) WB DyLight 405 Rat IgG2a Log in to see ICR10

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1173
(147), (138), (64), (64), (60), (58), (57), (54), (54), (53), (49), (49), (43), (37), (36), (33), (30), (30), (29), (27), (26), (25), (25), (23), (22), (19), (19), (18), (18), (16), (16), (16), (16), (15), (15), (15), (15), (15), (14), (14), (13), (11), (9), (9), (8), (8), (8), (7), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(2416), (864), (699), (28), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1458), (856), (81), (44), (9), (3), (2), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is un-conjugated
(102), (80), (71), (63), (56), (54), (32), (32), (28), (28), (28), (28), (28), (28), (27), (26), (26), (25), (25), (24), (24), (21), (21), (21), (21), (21), (20), (19), (18), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1444), (678), (615), (554), (508), (272), (259), (228), (124), (84), (65), (55), (40), (24), (19), (14), (12), (11), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Specificity Clone 9H2 specifically interacts with the 1170 - N A E pY L R V motif corresponding to the major autophosphorylation site of Human EGFR. Antibody AM00042PU-N does not crossreact with the highly homologous pTyr1248 of acticated ErbB2.
Characteristics Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1
Purification Purified from Serum-Free Cell Culture Supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Components incl. positive Control
Immunogen Phosphopeptide conjugated to KLHEpitope: phospho-Y1197 N A E pY L R V
Clone 9H2
Isotype IgG1

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR / ERBB1 (EGFR Antibody Abstract)
Background EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1
Gene ID 1956
UniProt P00533
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Usecelllysate delivered with this product as positive control: see protocol below! Immunoprecipitation (use at 1-10 μg per 10^6 vanadate-treated A431 cells). Immunocytochemistry (use at 1-10 μg/mL). Immunohistochemistry on Frozen and Paraffin Embedded Material. ELISA (use at 0.05 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control cell lysate provided: HepG2 Pervanadate Treated-recommended for ImmunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withpervanadate. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoid repeated freezing and thawing. Application: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysate contains SDS and is not recommended for applications withnative proteins such as immunoprecipitation !
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Reconstitution Restore with 1.0 mL H2O for 15 min at RT
Buffer PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freeze / thaw cycles!
Expiry Date 12 months

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN126779) anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody