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anti-Mouse (Murine) EGFR antibody for Immunoprecipitation

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • C-erb
  • CG10079
  • D-EGFR
  • D-Egf
  • DEGFR
  • DER
  • DER flb
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DEgfr
  • Degfr
  • Der
  • DmHD-33
  • Dmel\\CG10079
  • EFG-R
  • EGF-R
  • EGFR
  • EGFr
  • EGfr
  • EK2-6
  • Egf
  • Egf-r
  • EgfR
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • HD-33
  • TOP
  • Torpedo/DER
  • Torpedo/Egfr
  • c-erbB
  • d-egf-r
  • dEGFR
  • dEGFR1
  • dEgfr
  • der
  • egfr
  • flb
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57EFa
  • l(2)57Ea
  • mor1
  • top
  • top/DER
  • top/flb
  • torpedo/Egfr
  • torpedo/egfr
  • EGFR12
  • EGFR15
  • egfr1
  • Erbb2
  • ERBB
  • ERBB1
  • HER1
  • PIG61
  • mENA
  • ErbB-1
  • Errp
  • 9030024J15Rik
  • AI552599
  • Erbb
  • Errb1
  • Wa5
  • wa-2
  • wa2
  • epidermal growth factor receptor
  • Epidermal growth factor receptor
  • epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • EGFR
  • Egfr
  • egfra
  • egfr1
  • AaeL_AAEL004319
  • LOC100008806
Epitope
C-Term
148
120
61
58
54
53
52
50
48
48
48
43
36
35
33
30
27
26
25
25
24
23
22
21
19
18
18
17
17
17
16
16
16
15
15
15
15
15
15
14
13
13
11
9
9
8
7
7
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2241
843
697
29
24
18
17
15
15
6
3
2
2
2
1
1
1
Host
Rabbit
1382
760
67
43
8
2
1
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
97
74
66
59
50
48
26
26
25
24
24
24
23
23
23
23
23
23
23
23
21
21
21
21
21
20
19
19
18
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1304
594
557
483
437
272
221
221
108
84
66
55
35
24
19
13
11
8
6
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2854754
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126767 EIA IF IP WB Mouse IgG1 pSer1071 Log in to see 1H9 1
1 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
1 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
1 ABIN967696 IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
1 ABIN967697 FACS IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
1 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
1 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126785 EIA IF IP WB Biotin Mouse IgG1 C-Term Log in to see 13G8
1 ABIN126765 EIA IP WB Mouse IgG1 pTyr1069 Log in to see 11C2
1 ABIN152028 ICC IF IHC IHC (p) IP PLA WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN126786 EIA IF IP WB Mouse IgG1 C-Term Log in to see 13G8
1 ABIN126759 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr869 Log in to see 12A3
1 ABIN126768 EIA IP WB Biotin Mouse IgG1 pTyr1092 Log in to see 15A2
1 ABIN126756 EIA IP WB Biotin Mouse IgG1 pThr678 Log in to see 3F2
1 ABIN126766 EIA IF IP WB Biotin Mouse IgG1 pSer1071 Log in to see 1H9
1 ABIN126780 EIA IF IP WB Mouse IgG1 Tyr1197 Log in to see 20G3
1 ABIN126776 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr1173 Log in to see 9H2
1 ABIN266917 IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-EGFR antibody for Immunoprecipitation

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 4 Antibodies
ELISA 160 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 66 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 74 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 220 Antibodies
Immunohistochemistry (IHC) 185 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 158 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 548 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope C-Term
(148), (120), (61), (58), (54), (53), (52), (50), (48), (48), (48), (43), (36), (35), (33), (30), (27), (26), (25), (25), (24), (23), (22), (21), (19), (18), (18), (17), (17), (17), (16), (16), (16), (15), (15), (15), (15), (15), (15), (14), (13), (13), (11), (9), (9), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2241), (843), (697), (29), (24), (18), (17), (15), (15), (6), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(1382), (760), (67), (43), (8), (2), (1)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(97), (74), (66), (59), (50), (48), (26), (26), (25), (24), (24), (24), (23), (23), (23), (23), (23), (23), (23), (23), (21), (21), (21), (21), (21), (20), (19), (19), (18), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1304), (594), (557), (483), (437), (272), (221), (221), (108), (84), (66), (55), (35), (24), (19), (13), (11), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor)
EGFR antibody [C2C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Isoform 2: Secreted
Molecular Weight 134 kDa180
Gene ID 1956
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:5000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:5000-1:20000*
Comment

Positive Control: A431 , *Beas-2 , *A549 , *SiHa , *Hela , *Huh7 , *MSP ,

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A...
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) IP Image EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experimen...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) IHC-P Image EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human ga...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) WB Image Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE antibody diluted at ...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) IHC-P Image EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human ga...