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anti-Mouse (Murine) EGFR antibody for Immunoprecipitation

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
C-Term
145
138
59
57
54
52
52
49
47
46
45
44
36
34
32
31
30
26
25
24
24
24
23
22
22
19
18
17
17
16
16
14
14
14
14
14
14
14
13
13
13
11
10
10
10
9
9
9
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2196
763
625
23
16
14
13
10
8
5
4
2
1
1
1
1
1
Host
Rabbit
1397
698
69
45
7
2
Clonality
Polyclonal
Conjugate
This EGFR antibody is un-conjugated
94
74
64
57
46
44
44
25
23
21
21
21
21
21
21
21
20
20
19
19
19
19
19
19
19
19
16
2
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1332
577
526
447
427
252
244
184
87
73
73
55
25
11
11
8
6
6
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2854754
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008947584 ABIN126767 EIA IF IP WB Mouse IgG1 pSer1071 Log in to see 1H9 1
0.0008947584 ABIN126761 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr869 Log in to see 12A3
0.0008947584 ABIN126779 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG1 pTyr1173 Log in to see 9H2
0.0008947584 ABIN118051 EIA IHC (p) IP WB Rabbit AA 1168-1180 Log in to see Polyclonal 1
0.0008947584 ABIN152028 ICC IF IHC IHC (p) IP PLA WB Rabbit C-Term Log in to see Polyclonal 1
0.0008947584 ABIN967603 IHC (f) IP ELISA WB Mouse IgG1, kappa pTyr1173 Log in to see 9H2 3
0.0008947584 ABIN967696 IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
0.0008947584 ABIN967697 FACS IF IP IHC WB Mouse IgG1 AA 1020-1046 Log in to see 13-EGFR 5
0.0008947584 ABIN126757 EIA IP WB Mouse IgG1 pThr678 Log in to see 3F2
0.0008947584 ABIN126765 EIA IP WB Mouse IgG1 pTyr1069 Log in to see 11C2
0.0008947584 ABIN126770 EIA IP WB Mouse IgG1 pTyr1092 Log in to see 15A2
0.0008947584 ABIN126785 EIA IF IP WB Biotin Mouse IgG1 C-Term Log in to see 13G8
0.0008947584 ABIN126756 EIA IP WB Biotin Mouse IgG1 pThr678 Log in to see 3F2
0.0008947584 ABIN126759 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr869 Log in to see 12A3
0.0008947584 ABIN126768 EIA IP WB Biotin Mouse IgG1 pTyr1092 Log in to see 15A2
0.0008947584 ABIN126766 EIA IF IP WB Biotin Mouse IgG1 pSer1071 Log in to see 1H9
0.0008947584 ABIN126780 EIA IF IP WB Mouse IgG1 Tyr1197 Log in to see 20G3
0.0008947584 ABIN126786 EIA IF IP WB Mouse IgG1 C-Term Log in to see 13G8
0.0008947584 ABIN126776 EIA IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 pTyr1173 Log in to see 9H2
0.0008947584 ABIN492342 EIA IP WB Biotin Mouse IgG1 pTyr1069 Log in to see 11C2

Top referenced anti-EGFR antibody for Immunoprecipitation

Similar anti-EGFR Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 136 Antibodies
Enzyme Immunoassay (EIA) 23 Antibodies
Flow Cytometry (FACS) 67 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 32 Antibodies
Immunofluorescence (IF) 77 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 204 Antibodies
Immunohistochemistry (IHC) 185 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 131 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 482 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Blocking Peptide (BP) 8 Antibodies
Blocking Reagent (BR) 6 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope C-Term
(145), (138), (59), (57), (54), (52), (52), (49), (47), (46), (45), (44), (36), (34), (32), (31), (30), (26), (25), (24), (24), (24), (23), (22), (22), (19), (18), (17), (17), (16), (16), (14), (14), (14), (14), (14), (14), (14), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2196), (763), (625), (23), (16), (14), (13), (10), (8), (5), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1397), (698), (69), (45), (7), (2)
Clonality
Polyclonal
Conjugate This EGFR antibody is un-conjugated
(94), (74), (64), (57), (46), (44), (44), (25), (23), (21), (21), (21), (21), (21), (21), (21), (20), (20), (19), (19), (19), (19), (19), (19), (19), (19), (16), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1332), (577), (526), (447), (427), (252), (244), (184), (87), (73), (73), (55), (25), (11), (11), (8), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to EGFR (epidermal growth factor receptor)
EGFR antibody [C2C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human EGFR. The exact sequence is proprietary.
Isotype IgG

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGFR (EGFR Antibody Abstract)
Background The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Cellular Localization: Cell membrane, Single-pass type I membrane protein , Isoform 2: Secreted
Molecular Weight 134 kDa180
Gene ID 1956
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:5000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:5000-1:20000*
Comment

Positive Control: A431 , *Beas-2 , *A549 , *SiHa , *Hela , *Huh7 , *MSP ,

Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 40 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A...
Immunoprecipitation (IP) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) IP Image EGFR antibody [C2C3], C-term immunoprecipitates EGFR protein in IP experimen...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) IHC-P Image EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human ga...
Western Blotting (WB) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) WB Image Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE antibody diluted at ...
Immunohistochemistry (IHC) image for anti-EGFR antibody (Epidermal Growth Factor Receptor) (C-Term) (ABIN2854754) IHC-P Image EGFR antibody [C2C3], C-term detects EGFR protein at membrane on human ga...