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anti-Rat (Rattus) EGFR antibody for Intracellular Staining

Recommended EGFR Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor Receptor (EGFR) Antibodies
  • 9030024J15Rik
  • AI552599
  • C-erb
  • c-erbB
  • CG10079
  • D-Egf
  • d-egf-r
  • D-EGFR
  • Degfr
  • dEgfr
  • DEgfr
  • DEGFR
  • dEGFR
  • dEGFR1
  • der
  • DER
  • Der
  • DER/EGFR
  • DER/faint little ball
  • DER/top
  • DER/torpedo
  • DER1
  • DER flb
  • Dmel\\CG10079
  • DmHD-33
  • EFG-R
  • Egf
  • EGF-R
  • Egf-r
  • EgfR
  • EGfr
  • EGFr
  • EGFR
  • egfr
  • egfr1
  • EGFR12
  • EGFR15
  • EK2-6
  • El
  • Elp
  • Elp-1
  • Elp-B1
  • Elp-B1RB1
  • ERBB
  • Erbb
  • ErbB-1
  • ERBB1
  • Erbb2
  • Errb1
  • Errp
  • flb
  • HD-33
  • HER1
  • l(2)05351
  • l(2)09261
  • l(2)57DEFa
  • l(2)57Ea
  • l(2)57EFa
  • mENA
  • mor1
  • PIG61
  • TOP
  • top
  • top/DER
  • top/flb
  • Torpedo/DER
  • Torpedo/Egfr
  • torpedo/Egfr
  • torpedo/egfr
  • wa-2
  • wa2
  • Wa5
Epitope
pTyr1173
127
56
55
52
51
49
48
45
45
44
44
33
32
30
30
25
23
23
23
22
21
19
18
17
16
15
14
13
13
13
13
13
13
13
13
11
10
10
10
9
9
9
8
8
8
8
8
7
7
7
6
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
1700
661
589
20
14
14
12
10
7
7
4
2
1
1
1
Host
Mouse
1264
392
36
25
4
2
Clonality
Monoclonal
Conjugate
This EGFR antibody is conjugated to Alexa Fluor 647
74
57
45
35
25
25
22
22
21
21
21
21
21
21
20
2
2
1
Application
Intracellular Staining (ICS)
1103
500
380
240
232
205
175
142
86
60
55
54
25
11
9
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1177051
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676689 ABIN1177050 ICS Alexa Fluor 647 Mouse IgG1, kappa pTyr845 Log in to see 12A3 1

Top referenced anti-EGFR antibody for Intracellular Staining

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Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 1109 Antibodies 444 Antibodies 378 Antibodies 16 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Neutralization (Neut) 3 Antibodies
Luminex Assay (LMNX) 11 Antibodies
Intracellular Staining (ICS) 2 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Inhibition Assay (InhA) 2 Antibodies
Indirect Immunofluorescence Assay (IFA) 2 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Epidermal Growth Factor Receptor (EGFR) Antibodies
Epitope pTyr1173
(127), (56), (55), (52), (51), (49), (48), (45), (45), (44), (44), (33), (32), (30), (30), (25), (23), (23), (23), (22), (21), (19), (18), (17), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (13), (11), (10), (10), (10), (9), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(1700), (661), (589), (20), (14), (14), (12), (10), (7), (7), (4), (2), (1), (1), (1)
Host Mouse
(1264), (392), (36), (25), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EGFR antibody is conjugated to Alexa Fluor 647
(74), (57), (45), (35), (25), (25), (22), (22), (21), (21), (21), (21), (21), (21), (20), (2), (2), (1)
Application Intracellular Staining (ICS)
(1103), (500), (380), (240), (232), (205), (175), (142), (86), (60), (55), (54), (25), (11), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGFR Antibody

Target Details EGFR Application Details Handling References for anti-EGFR Antibody (ABIN1177051) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human EGF Receptor Peptide
Clone 9H2
Isotype IgG1, kappa

Target Details EGFR

Product Details anti-EGFR Antibody Application Details Handling References for anti-EGFR Antibody (ABIN1177051) Images back to top
Antigen
Alternative Name EGF Receptor (EGFR Antibody Abstract)
Background Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF Receptor (EGFR) binding and activation of intrinsic tyrosine kinase activity. EGFR, also known as ErbB1 or HER1, is a member of the ErbB class of receptor protein tyrosine kinases. It has an extracellular ligand-binding domain, a single transmembrane region, and a cytoplasmic region containing a protein tyrosine kinase domain and a c-terminal regulatory domain with many phosphorylation sites. Following ligand binding, EGFR forms homodimers and heterodimers with ErbB2. Specific C-terminal tyrosine residues are then autophosphorylated and, in turn, bind to adaptor proteins, kinases, or protein tyrosine phosphatases. Specifically, phosphorylated tyrosine 1173 (Y1173) interacts with Shc, SHP1, and PLCg, which mediate downstream signaling cascades and negative feedback regulation of EGFR activation. Inappropriate expression or mutations of EGFR and/or deregulation of its signaling pathways are associated with many types of cancer, making EGFR a promising target for cancer therapies. The 9H2 monoclonal antibody recognizes the phosphorylated Y1173 in the regulatory domain of human EGFR.
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-EGFR Antibody Target Details EGFR Handling References for anti-EGFR Antibody (ABIN1177051) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-EGFR Antibody Target Details EGFR Application Details References for anti-EGFR Antibody (ABIN1177051) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-EGFR Antibody (ABIN1177051)

Product Details anti-EGFR Antibody Target Details EGFR Application Details Handling Images back to top
Product cited in:

Olayioye, Neve, Lane, Hynes: "The ErbB signaling network: receptor heterodimerization in development and cancer." in: The EMBO journal, Vol. 19, Issue 13, pp. 3159-67, 2000