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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||1109 Antibodies||444 Antibodies||378 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Neutralization (Neut)||3 Antibodies|
|Luminex Assay (LMNX)||11 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies||2 Antibodies||2 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Inhibition Assay (InhA)||2 Antibodies|
|Indirect Immunofluorescence Assay (IFA)||2 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||215 Antibodies||36 Antibodies|
|Antigen||Epidermal Growth Factor Receptor (EGFR) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This EGFR antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-EGFR AntibodyTarget Details EGFR Application Details Handling Images
|Specificity||This antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr1173 (removal of the signal peptide).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details EGFRProduct Details anti-EGFR Antibody Application Details Handling Images back to top
|Alternative Name||EGFR / ERBB1 (EGFR Antibody Abstract)|
|Background||Epidermal growth factor mediates its effects on cell growth through its interaction with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGFalpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy-terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptorgulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1|
|Molecular Weight||approx. 175 kDa|
|Pathways||NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-EGFR Antibody Target Details EGFR Handling Images back to top
Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EGFR Antibody Target Details EGFR Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-EGFR Antibody Target Details EGFR Application Details Handling back to top