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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||12 Antibodies||18 Antibodies|
|Immunochromatography (IC)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies||10 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||10 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||13 Antibodies||16 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies||10 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies||5 Antibodies|
|Western Blotting (WB)||36 Antibodies||49 Antibodies|
|Antigen||Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This IKBKG antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-IKBKG AntibodyTarget Details IKBKG Application Details Handling References for anti-IKBKG Antibody (ABIN1002508) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN1004146|
|Purification||Affinity chromatography purified via peptide column.|
|Immunogen||Rabbit polyclol IKK( antibody was raised against a peptide (CQYQAPDMDTLQIHVME) corresponding to amino acids 400 to 416 of human IKK( , which are identical to those of mouse homologue .|
Target Details IKBKGProduct Details anti-IKBKG Antibody Application Details Handling References for anti-IKBKG Antibody (ABIN1002508) Images back to top
|Alternative Name||IkB Kinase gamma (IKBKG Antibody Abstract)|
|Background||Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The IkB kinase (IKKalpha and IKKbeta) phosphorylates IkB and mediates NF-kappaB activation. A novel molecule in the IKK complex was recently identified and termed IKKgamma/NEMO/FIP3/IKKAP1. IKKgamma interacts with IKKbeta and is required for the activation of IKK complex and NF-kappaB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kappaB activation.|
|Pathways||NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-IKBKG Antibody Target Details IKBKG Handling References for anti-IKBKG Antibody (ABIN1002508) Images back to top
|Application Notes||IKK-gamma antibody can be used for detection of IKK-gamma by Western blot at 0.5 to 1 µg/ml. (Optimal dilution should be determined by user.) A 52 kDa band should be detected. IKK-gamma has no cross response to IKK-alpha or IKK-beta. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-IKBKG Antibody Target Details IKBKG Application Details References for anti-IKBKG Antibody (ABIN1002508) Images back to top
|Buffer||Antibody is supplied in PBS containing 0.02% sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.|
|Expiry Date||12 months|
References for anti-IKBKG Antibody (ABIN1002508)Product Details anti-IKBKG Antibody Target Details IKBKG Application Details Handling Images back to top
|Product cited in:||
Li, Kang, Friedman et al.: "Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 3, pp. 1042-7, 1999 (PubMed).
Mercurio, Murray, Shevchenko et al.: "IkappaB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex." in: Molecular and cellular biology, Vol. 19, Issue 2, pp. 1526-38, 1999 (PubMed).
Rothwarf, Zandi, Natoli et al.: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." in: Nature, Vol. 395, Issue 6699, pp. 297-300, 1998 (PubMed).
Yamaoka, Courtois, Bessia et al.: "Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation." in: Cell, Vol. 93, Issue 7, pp. 1231-40, 1998 (PubMed).