anti-Mouse (Murine) IL1A antibody for Enzyme Immunoassay

Recommended IL1A Antibody (supplied by: Log in to see )

Antigen
Interleukin 1 alpha (IL1A) Antibodies
  • Il-1
  • Il-1a
  • IL-1 alpha
  • IL-1A
  • IL1
  • IL1-ALPHA
  • IL1F1
  • IL-1alpha
  • L1A
  • interleukin 1 complex
  • interleukin 1 alpha
  • interleukin 1, alpha
  • Il1
  • Il1a
  • IL1A
Reactivity
Mouse (Murine)
292
101
57
11
10
10
9
7
6
5
3
3
2
Host
Rabbit
233
162
34
9
7
7
5
3
3
Clonality
Polyclonal
Conjugate
This IL1A antibody is conjugated to Biotin
66
30
17
13
9
8
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
318
282
93
86
59
33
24
24
22
22
19
12
9
9
6
2
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN115859
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115860 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115862 EIA Func WB Rabbit Log in to see Polyclonal
1 ABIN115861 EIA Func WB Rabbit Log in to see Polyclonal
1 ABIN317141 EIA Func Mouse IgG1 Log in to see Flo1

General

Antigen Interleukin 1 alpha (IL1A) Antibodies
Reactivity Mouse (Murine)
(292), (101), (57), (11), (10), (10), (9), (7), (6), (5), (3), (3), (2)
Host Rabbit
(233), (162), (34), (9), (7), (7), (5), (3), (3)
Clonality
Polyclonal
Conjugate This IL1A antibody is conjugated to Biotin
(66), (30), (17), (13), (9), (8), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(318), (282), (93), (86), (59), (33), (24), (24), (22), (22), (19), (12), (9), (9), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1A Antibody

Target Details IL1A Application Details Handling Images
Specificity This antibody reacts with Mouse Interleukin-1 alpha (IL-1 alpha).
Purification Affinity chromatography
Immunogen Highly pure (>98%) recombinant mIL-1 alpha

Target Details IL1A

Product Details anti-IL1A Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-1 alpha / IL-1A (IL1A Antibody Abstract)
Background Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1
Gene ID 16175
NCBI Accession NP_034684
UniProt P01582
Pathways NF-kappaB Signaling, Autophagy

Application Details

Product Details anti-IL1A Antibody Target Details IL1A Handling Images back to top
Application Notes ELISA: (Direct): To detect mIL-1α by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2- 0.4 ng/well of recombinant mIL-1α. (Sandwich): To detect mIL-1α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine IL-1α (PP005P) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1α. Western blot: To detect mIL-1α by Western blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing
Restrictions For Research Use only

Handling

Product Details anti-IL1A Antibody Target Details IL1A Application Details Images back to top
Reconstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.