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Human IL1A ELISA Kit

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Antigen
Interleukin 1 alpha (IL1A) ELISA Kits
  • Il-1
  • IL-1 alpha
  • Il-1a
  • IL-1A
  • IL-1alpha
  • IL1
  • IL1-ALPHA
  • IL1F1
  • L1A
Epitope
AA 113-271
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
49
40
34
10
9
7
6
6
4
4
4
3
2
1
1
Methode Type
Sandwich ELISA
Detection Range
4.7-300 pg/mL
Minimum Detection Limit
4.7 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN411291
$ 260.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN2472115 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32693866 ABIN924776 Sandwich ELISA Cell Lysate, Serum, Plasma 8-1000 pg/mL 8 pg/mL Log in to see
0.32693866 ABIN414881 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32693866 ABIN1115633 Sandwich ELISA Serum, Plasma, Biological Fluids 3.906-250 pg/mL 3.91 pg/mL Log in to see
0.32693866 ABIN924896 Log in to see
0.32693866 ABIN1888520 Sandwich ELISA Cell Lysate, Tissue Lysate 0.5-300 pg/mL 0.5 pg/mL Log in to see
0.32693866 ABIN1888521 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 0.5-300 pg/mL 0.5 pg/mL Log in to see
0.32693866 ABIN2708253 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 3.9-250 pg/mL 3.9 pg/mL Log in to see
0.32693866 ABIN2708252 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 4.7-300 pg/mL 4.7 pg/mL Log in to see
0.32693866 ABIN2064441 Cell Culture Supernatant, Serum, Plasma Log in to see
0.32693866 ABIN2762268 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.32693866 ABIN2114185 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Log in to see
0.0012117105 ABIN365159 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Cell Lysate 31.25-2000 pg/mL 31.25 pg/mL Log in to see 1
0.0012117105 ABIN626501 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 10-250 pg/mL 10 pg/mL Log in to see
0.0012117105 ABIN1028976 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN625017 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 0.5-300 pg/mL 0.5 pg/mL Log in to see 1
0.0012117105 ABIN1112637 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 4.7-300 pg/mL 4.7 pg/mL Log in to see
0.0012117105 ABIN1446044 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples Log in to see
0.0012117105 ABIN625018 Sandwich ELISA Tissue Lysate, Cell Lysate 0.5-300 pg/mL 0.5 pg/mL Log in to see
0.0012117105 ABIN455548 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced IL1A ELISA Kit for ELISA

Similar IL1A ELISA Kits

Application / Reactivity Human
ELISA 48 ELISA Kits

General

Antigen Interleukin 1 alpha (IL1A) ELISA Kits
Epitope AA 113-271
Reactivity Human
Kits with alternative reactivity to:
(49), (40), (34), (10), (9), (7), (6), (6), (4), (4), (4), (3), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 4.7-300 pg/mL
Minimum Detection Limit 4.7 pg/mL
Application ELISA
Pubmed 11 references available
Supplier Log in to see

Product Details IL1A ELISA Kit

Target details Application Details Handling References for IL1A Kit (ABIN411291) Images
Purpose For quantitative detection of human IL-1 alpha in cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human IL-1 alpha
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <0.5pg/mL
Components
  • 96-well plate precoated with anti- human IL-1 alpha antibod - 1
  • Lyophilized recombinant human IL-1 alpha standar - 10ng/tubex2
  • Biotinylated anti- human IL-1 alpha antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: S113-A271

Target details

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Antigen
Alternative Name IL1A (IL1A ELISA Kit Abstract)
Background Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18 kDa.
Gene ID 3552
UniProt P01583
Pathways NF-kappaB Signaling, TCR Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S113-A271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1 alpha amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL human IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-1 alpha standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 54, Standard deviation: 1.84, CV(%): 3.4
  • Sample 2: n=16, Mean(pg/ml): 147, Standard deviation: 7.497, CV(%): 5.1
  • Sample 3: n=16, Mean(pg/ml): 221, Standard deviation: 9.28, CV(%): 4.2,
  • Sample 1: n=24, Mean(pg/ml): 48, Standard deviation: 2.64, CV(%): 5.5
  • Sample 2: n=24, Mean(pg/ml): 139, Standard deviation: 10.29, CV(%): 7.4
  • Sample 3: n=24, Mean(pg/ml): 234, Standard deviation: 14.98, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details IL1A ELISA Kit Target details Application Details References for IL1A Kit (ABIN411291) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL1A Kit (ABIN411291)

Product Details IL1A ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Han, Wang, Zhang, Liu, Li, Wang, Song, Pang, Ouyang, Tang: "Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NFκB pathways." in: Biology open, Vol. 4, Issue 5, pp. 661-5, 2015

Li, Huang, Shen: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." in: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, 2014

Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, 2014

Wang, Ji, Li, Han, Guo, Song, Quan, Wang, Han, Pang, Ouyang, Tang: "Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NF?B pathways." in: Scientific reports, Vol. 4, pp. 4905, 2014

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, 2014

Liu, Yang, Guo, Zhu, Shi, Wang, Qi, Guo, Ye, Yang, Liang: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." in: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, 2013

Duan, Liao, Huang, Nie, Wu: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." in: Clinical & developmental immunology, Vol. 2012, pp. 192791, 2012

Background publications

Diehl, Wang, Brooks, Burmeister, Califano, Wang, Schenkein: "Linkage disequilibrium of interleukin-1 genetic polymorphisms with early-onset periodontitis." in: Journal of periodontology, Vol. 70, Issue 4, pp. 418-30, 1999

Bailly, di Giovine, Blakemore, Duff: "Genetic polymorphism of human interleukin-1 alpha." in: European journal of immunology, Vol. 23, Issue 6, pp. 1240-5, 1993

Silver, Masson, George, Adam, Cox: "The IL-1 alpha and beta genes are closely linked (less than 70 kb) on mouse chromosome 2." in: Somatic cell and molecular genetics, Vol. 16, Issue 6, pp. 549-56, 1991

Modi, Masuda, Yamada, Oppenheim, Matsushima, OBrien: "Chromosomal localization of the human interleukin 1 alpha (IL-1 alpha) gene." in: Genomics, Vol. 2, Issue 4, pp. 310-4, 1989

Images

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Supplier Images
ELISA image for IL1A ELISA Kit (Interleukin 1 alpha) (ABIN411291) Human IL-1 alpha PicoKine ELISA Kit standard curve