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anti-Dog (Canine) IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
N-Term
34
26
20
18
17
16
13
12
9
9
7
7
6
6
5
5
5
5
5
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Bacteria, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
513
171
122
38
23
20
16
11
11
10
8
7
6
3
3
2
2
2
1
Host
Rabbit
400
342
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
502
411
152
126
115
70
66
46
35
29
24
24
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN269770
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12935057 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.12935057 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.12935057 ABIN1859379 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 103-260 Log in to see Polyclonal
0.12935057 ABIN3201448 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
0.12935057 ABIN4324364 ELISA IHC IHC (p) WB HRP Rabbit N-Term Log in to see Polyclonal
0.12935057 ABIN4324362 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011817255 ABIN1582289 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 3
0.0011817255 ABIN728503 IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 7
0.0011817255 ABIN964777 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0011817255 ABIN295086 FACS WB Biotin Goat IgG Log in to see Polyclonal
0.0011817255 ABIN2609647 WB FITC Rabbit IgG AA 103-260 Log in to see Polyclonal
0.0011817255 ABIN2623962 ELISA WB Biotin Rabbit IgG AA 103-260 Log in to see Polyclonal
0.0011817255 ABIN4324363 ELISA IHC IHC (p) WB Biotin Rabbit N-Term Log in to see Polyclonal
0.0011817255 ABIN2298238 FACS WB Biotin Goat IgG Log in to see Polyclonal
0.0011817255 ABIN2489521 IHC ELISA WB Rabbit Ala117, N-Term Log in to see Polyclonal
0.0011817255 ABIN2489520 IHC ELISA WB Rabbit Ala117, N-Term Log in to see Polyclonal
0.0011817255 ABIN2489522 IP Neut IHC ELISA WB Rabbit Ala117, N-Term Log in to see Polyclonal
0.0011817255 ABIN2489519 ELISA WB Rabbit Ala117, N-Term Log in to see Polyclonal
0.0011817255 ABIN2489523 IP Neut IHC ELISA WB Rabbit Ala117, N-Term Log in to see Polyclonal
0.0011817255 ABIN2692403 ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Rat (Rattus) Primate
Blocking Antibody (Inhibition) 3 Antibodies
Electron Microscopy (EM) 1 Antibodies 1 Antibodies
ELISA 61 Antibodies 12 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 10 Antibodies 3 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 38 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Neutralization (Neut) 16 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 92 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(34), (26), (20), (18), (17), (16), (13), (12), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bacteria, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
(513), (171), (122), (38), (23), (20), (16), (11), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1)
Host Rabbit
(400), (342), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(502), (411), (152), (126), (115), (70), (66), (46), (35), (29), (24), (24), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN269770) Images
Specificity Detects IL-1 beta and does not cross react with IL-1 alpha. Weakly detects the non-denatured 31 kDa precursor molecule but strongly detects all of the 17 kDa mature molecule. Additionally this IL-1 beta antibody will bind the denatured 31 kDa precursor molecule with greater affinity than the native form, but will often detect past the 35 kDa mark. This IL-1 beta antibody has also been shown to be useful in neutralization of human and primate IL-1 beta activity in bioassays.
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Immunogen Recombinant 153 aa human IL-1beta produced in E.coli. MW of recombinant IL-1beta 15kDa, with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1beta converting enzyme (ICE, capase-1).
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN269770) Images back to top
Antigen
Alternative Name IL-1 beta/il-1F2 (IL1B Antibody Abstract)
Background Gene Symbol: IL1B
Gene ID 3553, 16176, 24494
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN269770) Images back to top
Application Notes Western Blot 1:1000, ELISA 1:500-1:2000, Immunohistochemistry 1:100-1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:400-1:800, Immunohistochemistry-Paraffin 1:10-1:500, Electron Microscopy
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN269770) Images back to top
Format Liquid
Concentration 2.0 mg/mL
Buffer 0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN269770)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Ahmad, Chung, Tang, Hockman, Liu, Khan, Huo, Billings, Amar, Remaley, Manganiello: "Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue." in: Scientific reports, Vol. 6, pp. 28056, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Rossi, Cadamuro, Biselli-Périco, Leite, Severino, Reis, Cordeiro, Silva: "Interaction between inflammatory mediators and miRNAs in Helicobacter pylori infection." in: Cellular microbiology, Vol. 18, Issue 10, pp. 1444-58, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Su, Luo, Zhu, Fu, Zhao, Chen, Zhang, Ren, Yu, Yang, Wu, Feng, Li, Chen, Wang: "Interleukin-1β/Iinterleukin-1 receptor-associated kinase 1 inflammatory signaling contributes to persistent Gankyrin activation during hepatocarcinogenesis." in: Hepatology (Baltimore, Md.), Vol. 61, Issue 2, pp. 585-97, 2015 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Bimpis, Papalois, Voumvourakis, Oláh, Tiszlavicz, Liapi: "Neuronal tumour necrosis factor-α and interleukin-1β expression in a porcine model of intracerebral haemorrhage: Modulation by U-74389G." in: Brain research, Vol. 1615, pp. 98-105, 2015 (Sample species: Pig (Porcine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Arendt, McCready, Keller, Baker, Naber, Seewaldt, Kuperwasser: "Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis." in: Cancer research, Vol. 73, Issue 19, pp. 6080-93, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Paino, Ahlstrand, Nuutila, Navickaite, Lahti, Tuominen, Välimaa, Lamminmäki, Pöllänen, Ihalin: "Identification of a novel bacterial outer membrane interleukin-1Β-binding protein from Aggregatibacter actinomycetemcomitans." in: PLoS ONE, Vol. 8, Issue 7, pp. e70509, 2013 (Sample species: Bacteria). Further details: Western Blotting

Paino, Lohermaa, Sormunen, Tuominen, Korhonen, Pöllänen, Ihalin: "Interleukin-1β is internalised by viable Aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids." in: Cytokine, Vol. 60, Issue 2, pp. 565-74, 2012 (Sample species: Bacteria). Further details: Immunoelectron Microscopy,Immunohistochemistry

Petrovski, Ayna, Majai, Hodrea, Benko, Mádi, Fésüs: "Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1β release in human macrophages." in: Autophagy, Vol. 7, Issue 3, pp. 321-30, 2011 (Sample species: Human). Further details: Western Blotting

Paino, Tuominen, Jääskeläinen, Alanko, Nuutila, Asikainen, Pelliniemi, Pöllänen, Chen, Ihalin: "Trimeric form of intracellular ATP synthase subunit β of Aggregatibacter actinomycetemcomitans binds human interleukin-1β." in: PLoS ONE, Vol. 6, Issue 4, pp. e18929, 2011

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN269770) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) anti-Interleukin 1, beta (IL1B) (N-Term) antibody
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Lane 1: Human IL-1Beta. Load: ...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Antibody was used at a 1:200 d...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - pro-IL-1beta in glioblastoma c...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Western Blot: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Analysis of IL-1 beta in Rhesu...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Analysis of: Human IL1...
Immunofluorescence (IF) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN269770) Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [ABIN269770] - Trab...