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anti-Dog (Canine) IL1B antibody for Western Blotting

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Epitope
N-Term
30
29
26
20
15
15
15
14
12
11
10
7
7
7
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
510
176
125
37
26
20
16
13
12
11
8
7
6
4
4
2
2
2
1
1
Host
Rabbit
384
343
17
16
8
7
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
94
45
33
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
507
406
164
124
110
72
59
53
38
29
24
24
14
14
13
7
5
4
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN5326748
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0923712 ABIN269770 ELISA EM ICC IF IHC IHC (p) IP WB Rabbit N-Term Log in to see Polyclonal 9
3.0923712 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
3.0923712 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
3.0923712 ABIN4324364 ELISA IHC IHC (p) WB HRP Rabbit N-Term Log in to see Polyclonal
3.0923712 ABIN1859379 ICC IHC IP WB Rabbit IgG AA 103-260 Log in to see Polyclonal
3.0923712 ABIN4324362 ELISA WB Rabbit N-Term Log in to see Polyclonal 1
3.0923712 ABIN3201448 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
1 ABIN1582289 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 3
1 ABIN728503 IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 11
1 ABIN964777 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
1 ABIN4899222 FACS Neut WB Goat IgG AA 114-265 Log in to see Polyclonal 1
1 ABIN1102518 FACS ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1102517 FACS IHC ELISA WB HRP Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1102516 FACS IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN799612 IHC (fro) IHC (p) IP RIA ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2623962 ELISA WB Biotin Rabbit IgG AA 103-260 Log in to see Polyclonal
1 ABIN2609647 ELISA WB FITC Rabbit IgG AA 103-260 Log in to see Polyclonal
1 ABIN295086 FACS WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN4899221 FACS WB Biotin Goat IgG AA 114-265 Log in to see Polyclonal
1 ABIN2609632 ELISA WB Rabbit IgG AA 103-260 Log in to see Polyclonal

Top referenced anti-IL1B antibody for Western Blotting

Similar anti-IL1B Antibodies

Application / Reactivity Human Mouse (Murine) Rabbit Dog (Canine) Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 304 Antibodies 126 Antibodies 26 Antibodies 12 Antibodies 12 Antibodies
Radioimmunoassay (RIA) 9 Antibodies 4 Antibodies 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Neutralization (Neut) 29 Antibodies 32 Antibodies 4 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope N-Term
(30), (29), (26), (20), (15), (15), (15), (14), (12), (11), (10), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit
(510), (176), (125), (37), (26), (20), (16), (13), (12), (11), (8), (7), (6), (4), (4), (2), (2), (2), (1), (1)
Host Rabbit
(384), (343), (17), (16), (8), (7), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(94), (45), (33), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(507), (406), (164), (124), (110), (72), (59), (53), (38), (29), (24), (24), (14), (14), (13), (7), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN5326748) Images
Blocking Peptide Blocking peptide for this product available: ABIN978485
Sequence MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL
Predicted Reactivity Dog: 92%, Horse: 86%, Human: 100%, Mouse: 75%, Rabbit: 92%
Characteristics This is a rabbit polyclonal antibody against IL1B. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN5326748) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Alias Symbols: IL-1, IL1-BETA, IL1F2
Protein Interaction Partner: PTPN1, LYN, FYN, CMA1, CASP1, APP, SP1, EGR1, ELAVL1, CASP4, IL1RAP, PRTN3, IL1R2, IL1R1, IL1B, MMP2, ADRB2, A2M, UBE2N, ZNF675, MAPK8IP2,
Protein Size: 269
Molecular Weight 17 kDa
Gene ID 3553
NCBI Accession NM_000576, NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN5326748) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 269 AA

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN5326748) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL1B Antibody (ABIN5326748)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Product cited in:

Wang, Jiang, Li, He, Zhao, Yang, Zhu, Yin, Li, Wang, Liu, Shang, Yang, Wang, Zhen, Zhang, Yu, Zhong, Ye, Huang, Yuan: "Shigella flexneri T3SS effector IpaH4.5 modulates the host inflammatory response via interaction with NF-κB p65 protein." in: Cellular microbiology, Vol. 15, Issue 3, pp. 474-85, 2013

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN5326748) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN5326748) WB Suggested Anti-IL1B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN5326748) Sample Type: Human Macrophange Cells
Green: primary
Red: p...
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (N-Term) (ABIN5326748) WB Suggested Anti-IL1B
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500