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anti-Goat IL1B antibody for Flow Cytometry

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Cow (Bovine), Goat, Sheep (Ovine)
503
170
121
37
23
19
16
11
11
10
8
7
6
3
2
2
2
1
Host
Mouse
399
332
20
14
8
8
6
4
3
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
100
49
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
499
402
147
122
112
70
63
46
31
28
24
21
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120889
$ 764.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010485112 ABIN2474895 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 1D4 4

Top referenced anti-IL1B antibody for Flow Cytometry

Similar anti-IL1B Antibodies

Application / Reactivity Sheep (Ovine) Goat Cow (Bovine)
ELISA 24 Antibodies 2 Antibodies 15 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies 3 Antibodies
Flow Cytometry (FACS) 6 Antibodies 2 Antibodies 2 Antibodies
Functional Studies (Func) 1 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 2 Antibodies 3 Antibodies
Western Blotting (WB) 24 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Cow (Bovine), Goat, Sheep (Ovine)
(503), (170), (121), (37), (23), (19), (16), (11), (11), (10), (8), (7), (6), (3), (2), (2), (2), (1)
Host Mouse
(399), (332), (20), (14), (8), (8), (6), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(100), (49), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(499), (402), (147), (122), (112), (70), (63), (46), (31), (28), (24), (21), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN120889) Images
Purification Purified
Immunogen Recombinant ovine IL-1 Beta
Clone 1D4
Isotype IgG1

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN120889) Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN120889) Images back to top
Application Notes ELISA: 5 μg/mL as coating antibody. Flow Cytometry: 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN120889) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months

References for anti-IL1B Antibody (ABIN120889)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Rothel, Hurst, Seow, Pépin, Berthon, Corner, Wood: "Analysis of ovine IL-1 beta production in vivo and in vitro by enzyme immunoassay and immunohistochemistry." in: Veterinary immunology and immunopathology, Vol. 57, Issue 3-4, pp. 267-78, 1997