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anti-Human IL1B antibody for Enzyme Immunoassay

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
Middle Region
33
26
20
18
17
16
13
12
9
9
7
7
6
6
5
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
502
170
121
38
23
20
16
11
11
10
8
7
6
3
3
2
2
1
Host
Rabbit
398
333
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
49
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
498
402
147
123
112
70
64
46
31
28
24
21
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN453702
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN1107772 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3A6 2
0.0008870071 ABIN112300 ELISA (Detection) EIA Mouse IgM Log in to see 5C10
0.0008870071 ABIN114529 EIA WB Mouse IgG1 Log in to see S1H12
0.0008870071 ABIN264349 EIA Biotin Mouse IgG1 Log in to see AS57
0.0008870071 ABIN264346 EIA Func IHC (fro) IP Mouse IgG1 Log in to see AS10
0.0008870071 ABIN453901 EIA Mouse IgG1 Log in to see S1B7
0.0008870071 ABIN453904 EIA Mouse IgG1 Log in to see S3F12
0.0008870071 ABIN116862 EIA Func IHC (fro) IHC (p) IP RIA WB Rabbit Log in to see Polyclonal 3
0.0008870071 ABIN264358 EIA Mouse IgG1 Log in to see AS56
0.0008870071 ABIN116861 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal 2
0.0008870071 ABIN1107771 EIA Mouse IgG1 Log in to see 464
0.0008870071 ABIN1803663 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal
0.0008870071 ABIN1803662 EIA RIA Rabbit IgG Log in to see Polyclonal
0.0008870071 ABIN2723571 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL1B antibody for Enzyme Immunoassay

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Application / Reactivity Human
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ELISA 222 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 97 Antibodies
Functional Studies (Func) 8 Antibodies
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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope Middle Region
(33), (26), (20), (18), (17), (16), (13), (12), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(502), (170), (121), (38), (23), (20), (16), (11), (11), (10), (8), (7), (6), (3), (3), (2), (2), (1)
Host Rabbit
(398), (333), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(100), (49), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(498), (402), (147), (123), (112), (70), (64), (46), (31), (28), (24), (21), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN453702) Images
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide selected from the Center region of human IL1B

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN453702) Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 3553
NCBI Accession NP_000567
UniProt P01584
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN453702) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN453702) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL1B Antibody (ABIN453702)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Ito, Mukaiyama, Itoh, Nagase, Thogersen, Enghild, Sasaguri, Mori: "Degradation of interleukin 1beta by matrix metalloproteinases." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14657-60, 1996

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN453702) back to top
Supplier Images
Western Blotting (WB) image for anti-IL1B antibody (Interleukin 1, beta) (Middle Region) (ABIN453702) Western blot analysis of IL1B Antibody (Center) in NCI-H460 cell line lysates (35ug/l...