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anti-Human IL1B antibody for ELISA

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Epitope
AA 170-269
23
20
18
17
16
13
12
10
9
9
7
7
6
6
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
338
145
102
38
20
20
16
11
10
8
8
7
6
3
3
2
2
1
Host
Mouse
376
183
19
14
8
8
6
4
3
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
90
45
25
13
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
377
350
123
91
63
45
34
29
29
28
24
18
14
13
12
7
5
4
4
4
3
3
1
1
1
1
1
1
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Catalog No. ABIN395309
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31757757 ABIN969215 ICC IHC ELISA WB Mouse IgG1 Log in to see 3A6 2
0.31757757 ABIN516996 IF IP ELISA WB Mouse IgG2b kappa AA 170-269, partial Log in to see 2A8 1
0.31757757 ABIN263126 ELISA Biotin Mouse IgG2b kappa Log in to see JK1B-2
0.31757757 ABIN2467945 ELISA WB Chicken AA 118-269 Log in to see Polyclonal
0.31757757 ABIN263124 ICC FACS IF ELISA Mouse IgG1 Log in to see JK1B-1
0.31757757 ABIN263127 ELISA Mouse IgG2b kappa Log in to see JK1B-2
0.31757757 ABIN516997 ELISA WB Mouse IgG2a kappa AA 170-269, partial Log in to see 1G8
0.31757757 ABIN516998 IF ELISA WB Mouse IgG1 kappa AA 1-269, full length Log in to see 2E8
0.31757757 ABIN1845349 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
0.0012704609 ABIN1582289 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 3
0.0012704609 ABIN964768 FACS IP IHC Neut RIA ELISA WB HRP Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0012704609 ABIN1536090 IHC ELISA WB Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
0.0012704609 ABIN1536091 IHC ELISA Rabbit IgG Cleaved-Asp210 Log in to see Polyclonal
0.0012704609 ABIN964752 FACS IP IHC Neut RIA ELISA WB Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0012704609 ABIN288401 IHC ELISA WB Biotin Goat IgG Log in to see Polyclonal
0.0012704609 ABIN1498871 ICC IF IHC ELISA WB Mouse IgG1 Log in to see 3A6
0.0012704609 ABIN964777 FACS IP IHC Neut RIA ELISA WB Biotin Rabbit IgG N-Term, Cleavage Site Log in to see Polyclonal
0.0012704609 ABIN256252 ELISA Biotin Mouse IgG1 kappa Log in to see H1b-98
0.0012704609 ABIN284570 FACS IHC ELISA WB Mouse IgG1 Log in to see 9627
0.0012704609 ABIN1102516 FACS IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-IL1B antibody for ELISA

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Application / Reactivity Human
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Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA 179 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 67 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 99 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Epitope AA 170-269
(23), (20), (18), (17), (16), (13), (12), (10), (9), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(338), (145), (102), (38), (20), (20), (16), (11), (10), (8), (8), (7), (6), (3), (3), (2), (2), (1)
Host Mouse
(376), (183), (19), (14), (8), (8), (6), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(90), (45), (25), (13), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(377), (350), (123), (91), (63), (45), (34), (29), (29), (28), (24), (18), (14), (13), (12), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN395309) Images
Specificity IL1B (AAH08678, 170 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen IL1B (AAH08678, 170 a.a. ~ 269 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2A8
Isotype IgG2b kappa

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN395309) Images back to top
Antigen
Alternative Name IL1B (IL1B Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 30748 DA
Gene ID 12406
Research Area Cell Cycle, Cell Structure
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN395309) Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN395309) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-IL1B Antibody (ABIN395309)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

De Iudicibus, Stocco, Martelossi, Londero, Ebner, Pontillo, Lionetti, Barabino, Bartoli, Ventura, Decorti: "Genetic predictors of glucocorticoid response in pediatric patients with inflammatory bowel diseases." in: Journal of clinical gastroenterology, Vol. 45, Issue 1, pp. e1-7, 2010

Romero, Friel, Velez Edwards, Kusanovic, Hassan, Mazaki-Tovi, Vaisbuch, Kim, Erez, Chaiworapongsa, Pearce, Bartlett, Salisbury, Anant, Vovis, Lee, Gomez, Behnke, Oyarzun, Tromp, Williams, Menon: "A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM)." in: American journal of obstetrics and gynecology, Vol. 203, Issue 4, pp. 361.e1-361.e30, 2010

Liu, Chen, Wu, Liao, Chen, Tsai, Tsai, Wan, Tsai: "Association of interleukin-1beta (IL1B) polymorphisms with Graves' ophthalmopathy in Taiwan Chinese patients." in: Investigative ophthalmology & visual science, Vol. 51, Issue 12, pp. 6238-46, 2010

Cheng, Chang, Wang, Wang: "Interleukin-1beta and -10 polymorphisms influence erosive reflux esophagitis and gastritis in Taiwanese patients." in: Journal of gastroenterology and hepatology, Vol. 25, Issue 8, pp. 1443-51, 2010

Chen, Cheng: "[Correlation of IL-1beta-511 and IL-1RN VNTR gene polymorphism to coronary heart disease: a meta-analysis]." in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University, Vol. 30, Issue 7, pp. 1635-8, 2010

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN395309) back to top
Supplier Images
ELISA image for anti-IL1B antibody (Interleukin 1, beta) (AA 170-269) (ABIN395309) Detection limit for recombinant GST tagged IL1B is approximately 10 ng/mL as a captur...