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anti-Human IL1B antibody for ELISA (Capture)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Human
511
171
122
38
24
20
17
12
11
10
8
7
6
3
3
2
2
2
1
1
Host
Mouse
400
340
20
14
8
8
6
4
3
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture), Flow Cytometry (FACS), Intracellular Staining (ICS), Neutralization (Neut)
501
411
151
125
115
69
66
47
35
29
25
24
13
13
12
7
6
5
4
4
4
3
3
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN281605
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN191246 ELISA (Capture) ELISA Mouse IgG2b Log in to see 1E6 2
0.0008709465 ABIN191248 ELISA (Capture) ELISA Mouse IgG2a Log in to see 6E10 2
0.0008709465 ABIN282501 ELISA (Capture) ELISpot Neut Mouse IgG1 Log in to see CRM56
0.0008709465 ABIN2772611 ELISA (Capture) IF ELISA WB Mouse IgG1, kappa Log in to see

Top referenced anti-IL1B antibody for ELISA (Capture)

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General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human
(511), (171), (122), (38), (24), (20), (17), (12), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(400), (340), (20), (14), (8), (8), (6), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), Flow Cytometry (FACS), Intracellular Staining (ICS), Neutralization (Neut)
(501), (411), (151), (125), (115), (69), (66), (47), (35), (29), (25), (24), (13), (13), (12), (7), (6), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity The CRM56 antibody reacts with human and baboon interleukin-1 beta, (IL-1 beta). IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.
Characteristics The CRM56 antibody reacts with human and baboon interleukin-1 beta, (IL-1 beta). IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.
Synonyms: Synonyms: Interleukin-1 beta
Purification Affinity Purified
Clone CRM56
Isotype IgG1

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-1b (IL1B Antibody Abstract)
Background IL-1beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Applications reported: The CRM56 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry. Functional Grade purified CRM56 is recommended for use in functional assays.

Applications tested: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1 beta (IL-1 beta) in combination with the biotin CRM57 (13-7016) antibody for detection and recombinant human IL-1 beta (14-8018) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The Functional Grade Purified CRM56 antibody has been tested for neutralization of IL-1 beta bioactivity.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C

Images

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling back to top
Supplier Images
 image for anti-IL1B antibody (Interleukin 1, beta) (ABIN281605) Human peripheral blood mononuclear cells were stimulated with LPS in the presence of ...
 image for anti-IL1B antibody (Interleukin 1, beta) (ABIN281605) anti-Interleukin 1, beta (IL1B) antibody (Image 2)