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anti-Human IL1B antibody for ELISpot

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Human
513
172
123
38
24
20
17
12
11
10
8
7
6
3
3
2
2
2
1
1
Host
Mouse
401
341
20
14
8
8
6
4
3
Clonality
Monoclonal
Conjugate
This IL1B antibody is un-conjugated
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA (Capture), ELISpot, Neutralization (Neut)
503
412
153
126
116
69
66
47
36
29
25
24
14
13
12
7
6
5
4
4
3
3
3
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN282501
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008707584 ABIN1724560 ELISpot Func FACS ELISA Mouse IgG1 Log in to see B-A15
0.0008707584 ABIN279961 ELISA (Detection) ELISpot Biotin Mouse IgG2b Log in to see CRM57
0.0008707584 ABIN1448853 ELISpot Log in to see

Similar anti-IL1B Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA 221 Antibodies
ELISA (Capture) 5 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 95 Antibodies
Functional Studies (Func) 8 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Human
(513), (172), (123), (38), (24), (20), (17), (12), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Mouse
(401), (341), (20), (14), (8), (8), (6), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL1B antibody is un-conjugated
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Capture), ELISpot, Neutralization (Neut)
(503), (412), (153), (126), (116), (69), (66), (47), (36), (29), (25), (24), (14), (13), (12), (7), (6), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Specificity The CRM56 antibody reacts with human and baboon interleukin-1 beta, (IL-1 beta). IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.
Characteristics The CRM56 antibody reacts with human and baboon interleukin-1 beta, (IL-1 beta). IL-1 beta is a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.
Synonyms: Synonyms: Interleukin-1 beta
Purification Affinity Purified
Endotoxin Level <0.001 ng endotoxin/μg
Grade Functional Grade
Clone CRM56
Isotype IgG1

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-1b (IL1B Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes Applications reported: The CRM56 antibody has been reported for use in cytokine neutralization, and ELISA. Fluorochrome conjugated CRM56 is recommended for use in intracellular staining for flow cytometry. Functional Grade purified CRM56 antibody, cat. 16-7018, is recommended for use in functional assays.

Applications tested: The CRM56 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interleukin-1ß (IL-1ß) in combination with the biotin CRM57 (13-7016) antibody for detection and recombinant human IL-1ß (14-8018) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1 - 4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The Functional Grade Purified CRM56 antibody has been tested by LAL assay to verify low endotoxin and by bioassay to verify neutralization of IL- 1ß bioactivity. For in vitro neutralization, the CRM56 antibody at 2.5 μg/mL has been found to neutralize by 50 % the effect of 1 ng/mL human IL-1ß bioactivity in a D10.G4.1 cell proliferation bioassay.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Concentration 1 mg/mL
Buffer aqueous buffer, no sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Use in sterile environment.
Storage 4 °C