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anti-Mouse (Murine) IL1B antibody for Enzyme Immunoassay

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Mouse (Murine)
512
170
122
38
24
20
17
12
11
10
8
7
6
3
3
2
2
2
1
1
Host
Rabbit
399
341
20
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
101
50
35
21
13
10
10
9
8
8
8
8
8
8
8
8
8
8
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
500
411
151
126
115
70
66
47
35
28
25
23
14
13
12
7
7
5
4
4
4
3
3
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN115865
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN616079 EIA IHC (fro) IF IHC (p) IP Neut RIA WB Rabbit Log in to see Polyclonal 3
0.0008867729 ABIN115863 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008867729 ABIN115864 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008867729 ABIN115866 EIA Func WB Rabbit Log in to see Polyclonal

Top referenced anti-IL1B antibody for Enzyme Immunoassay

Similar anti-IL1B Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 84 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Intracellular Staining (ICS) 6 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine)
(512), (170), (122), (38), (24), (20), (17), (12), (11), (10), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(399), (341), (20), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(101), (50), (35), (21), (13), (10), (10), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(500), (411), (151), (126), (115), (70), (66), (47), (35), (28), (25), (23), (14), (13), (12), (7), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN115865) Images
Purification Affinity chromatography.
Immunogen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-beta.

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN115865) Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 16176
NCBI Accession NP_032387
UniProt P10749
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN115865) Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofmIL-1-beta (50 pg/mL), a concentration of 100 - 150 ng/mL of this antibody is required. Elisa: To detect mIL-1-beta by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant mIL-1-beta. Western Blot: To detect mIL-1-beta by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-1-beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Centrifuge vial prior to opening.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN115865) Images back to top
Reconstitution Restore with sterile water to a concentration of 0.1-1.0 mg/mL.
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized antibody can be stored for one year from despatch at -20 °C. Reconstituted antibody is stable for two weeks at 2-8 °C, frozen aliquots are stable for six months at -20 °C.
Expiry Date 12 months

References for anti-IL1B Antibody (ABIN115865)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Yao, Karabasil, Purwanti, Li, Akamatsu, Kanamori, Hosoi: "Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1beta in the submandibular gland of mice." in: The Journal of biological chemistry, Vol. 281, Issue 12, pp. 7968-76, 2006

Gonzalez-Rey, Chorny, Robledo, Delgado: "Cortistatin, a new antiinflammatory peptide with therapeutic effect on lethal endotoxemia." in: The Journal of experimental medicine, Vol. 203, Issue 3, pp. 563-71, 2006