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anti-Mouse (Murine) IL1B antibody for Immunohistochemistry (Frozen Sections)

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • IL-1
  • IL-1B
  • Il-1b
  • IL-1beta
  • IL-1BETA
  • il1-b
  • IL1-BETA
  • IL1B
  • IL1beta
  • IL1F2
  • zgc:111873
Reactivity
Mouse (Murine), Rat (Rattus)
339
144
101
38
20
20
16
11
10
8
8
7
6
3
3
2
2
1
Host
Rabbit
375
184
19
14
8
8
6
4
3
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
90
45
25
13
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Radioimmunoassay (RIA), Western Blotting (WB)
377
351
123
91
62
44
33
29
28
27
24
17
14
12
12
7
5
4
4
4
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN616079
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN1859383 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 118-269 Log in to see Polyclonal
0.00088051456 ABIN1173157 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 118-269 Log in to see Polyclonal
0.00088051456 ABIN2474899 Func IHC (fro) ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.00088051456 ABIN1980439 ICC IHC (fro) IHC (p) ELISA WB Mouse AA 118-269 Log in to see

Top referenced anti-IL1B antibody for Immunohistochemistry (Frozen Sections)

Similar anti-IL1B Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies 2 Antibodies
ELISA 57 Antibodies 75 Antibodies
ELISA (Capture) 2 Antibodies 2 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Neutralization (Neut) 16 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 71 Antibodies
ELISA (Detection) 1 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(339), (144), (101), (38), (20), (20), (16), (11), (10), (8), (8), (7), (6), (3), (3), (2), (2), (1)
Host Rabbit
(375), (184), (19), (14), (8), (8), (6), (4), (3)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(90), (45), (25), (13), (5), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Radioimmunoassay (RIA), Western Blotting (WB)
(377), (351), (123), (91), (62), (44), (33), (29), (28), (27), (24), (17), (14), (12), (12), (7), (5), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling References for anti-IL1B Antibody (ABIN616079) Images
Specificity This antibody is primarily directed against mature, 17,000 MW Mouse IL1-beta and is useful in determining its presence in various assays. The antibody does not recognize Human IL1-beta or Mouse IL1-alpha. In ELISA formats and other immunoreactive assays, reactivity occurs with Rat IL1-beta. This antibody will recognize 10 % of the non-denatured (native) precursor 31,000 MW Mouse IL1-beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in Immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL1-beta precursor molecule. Denatured 31,000 precursor IL1-beta will be recognized by this antibody.
Purification DEAE Fractionation
Immunogen Recombinant Mouse IL-1 beta produced in E. coli Genename: IL1b Remarks: The MW of the recombinant Mouse IL-1 beta is 17 kDa

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling References for anti-IL1B Antibody (ABIN616079) Images back to top
Antigen
Alternative Name Interleukin-1 beta / IL-1B (IL1B Antibody Abstract)
Background IL-1 beta (also known as Interleukin-1 beta, IL1-beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2
Gene ID 16176
NCBI Accession NP_032387
UniProt P10749
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling References for anti-IL1B Antibody (ABIN616079) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details References for anti-IL1B Antibody (ABIN616079) Images back to top
Reconstitution Restore with 0.1 mL of deionized water (or equivalent)
Concentration 1.0 mg/mL (UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, None, None
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C or -20 °C. Following reconstitution store at -20 °C.

References for anti-IL1B Antibody (ABIN616079)

Product Details anti-IL1B Antibody Target Details IL1B Application Details Handling Images back to top
Background publications

Huang, Newton, Rutledge, Horuk, Matthew, Covington, Lin: "Characterization of murine IL-1 beta. Isolation, expression, and purification." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3838-43, 1988

Telford, Macchia, Massone, Carinci, Palla, Melli: "The murine interleukin 1 beta gene: structure and evolution." in: Nucleic acids research, Vol. 14, Issue 24, pp. 9955-63, 1987

Gray, Glaister, Chen, Goeddel, Pennica: "Two interleukin 1 genes in the mouse: cloning and expression of the cDNA for murine interleukin 1 beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 11, pp. 3644-8, 1986