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anti-Rat (Rattus) IL1B antibody for Blocking Antibody

Recommended IL1B Antibody (supplied by: Log in to see )

Antigen
Interleukin 1, beta (IL1B) Antibodies
  • il1-b
  • zgc:111873
  • IL-1beta
  • Il-1b
  • IL1B
  • IL-1
  • IL1-BETA
  • IL1F2
  • IL-1BETA
  • IL1beta
  • IL-1B
  • interleukin 1, beta
  • interleukin 1 beta
  • il1b
  • Il1b
  • IL-1B
  • IL1B
Reactivity
Rat (Rattus)
533
179
127
39
31
22
18
17
12
11
8
7
7
4
3
2
2
2
1
1
Host
Rabbit
408
351
17
16
8
8
6
3
2
Clonality
Polyclonal
Conjugate
This IL1B antibody is un-conjugated
100
48
35
22
13
12
11
10
8
8
8
8
8
8
8
8
8
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
539
433
176
126
110
74
59
58
38
29
25
24
14
14
13
7
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1002569
$ 374.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN289475 Inhibition ELISA WB Rabbit Log in to see Polyclonal
1 ABIN346634 Inhibition Rabbit Log in to see Polyclonal

Similar anti-IL1B Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Neutralization (Neut) 15 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Western Blotting (WB) 103 Antibodies

General

Antigen Interleukin 1, beta (IL1B) Antibodies
Reactivity Rat (Rattus)
(533), (179), (127), (39), (31), (22), (18), (17), (12), (11), (8), (7), (7), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(408), (351), (17), (16), (8), (8), (6), (3), (2)
Clonality
Polyclonal
Conjugate This IL1B antibody is un-conjugated
(100), (48), (35), (22), (13), (12), (11), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), ELISA, Western Blotting (WB)
(539), (433), (176), (126), (110), (74), (59), (58), (38), (29), (25), (24), (14), (14), (13), (7), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-IL1B Antibody

Target Details IL1B Application Details Handling Images
Purification Antigen affinity purified polyclol antibody
Sterility Sterile filtered
Immunogen recombint rIL-1beta (rat IL-1beta)
Isotype IgG

Target Details IL1B

Product Details anti-IL1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 1-beta (IL1B Antibody Abstract)
Background Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rIL-1beta (rat IL-1beta). Anti-Rat IL-1beta specific antibody was purified by affinity chromatography employing immobilized rIL-1beta matrix.
Pathways NF-kappaB Signaling, TCR Signaling, Interferon-gamma Pathway, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-IL1B Antibody Target Details IL1B Handling Images back to top
Application Notes To yield one-half maximal inhibition [ND₅₀] of the biological activity of Rat IL-1beta (0.20 ng/µl), a concentration of 0.09 - 0.14 µg/µl of this antibody is required. To detect rat IL-1beta by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat IL-1beta. To detect Rat IL1beta by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Rat IL1beta (PK-AB815-61120RB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL1beta. To detect Rat IL1beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL1beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunihostochemistry: NA. To detect Rat IL1beta by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PromoKine's Biotinylated Anti-Rat IL1beta (PK-AB815-61120RB) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL1beta. To detect Rat IL1beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL1beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL1B Antibody Target Details IL1B Application Details Images back to top
Format Lyophilized
Concentration To yield one-half maximal inhibition [ND₅₀] of the biological activity of Rat IL-1beta (0.20 ng/µl), a concentration of 0.09 - 0.14 µg/µl of this antibody is required.
Buffer A sterile-filtered antibody solution was lyophilized from PBS, pH 7.2.
Handling Advice The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening. Reconstitute in sterile water at a concentration of 0.1-1.0 mg/ml.
Storage -20 °C
Expiry Date 24 months