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|Application / Reactivity||Rat (Rattus)|
|ELISA||25 ELISA Kits|
|Antigen||Insulin (INS) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Competition ELISA|
|Detection Range||123.46-10000 pg/mL|
|Minimum Detection Limit||123.46 pg/mL|
|12 references available|
|Supplier||Log in to see|
Product Details Insulin ELISA KitTarget details Application Details Handling References for Insulin Kit (ABIN416266) Images
|Purpose||The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of INS in Serum,Plasma,Biological Fluids|
|Sample Type||Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids|
|Specificity||This assay has high sensitivity and excellent specificity for detection of Insulin (INS).|
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.|
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by subtracting two standard deviations - the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
|Material not included||
Target detailsProduct Details Insulin ELISA Kit Application Details Handling References for Insulin Kit (ABIN416266) Images back to top
|Alternative Name||INS (INS ELISA Kit Abstract)|
|Pathways||NF-kappaB Signaling, RTK Signaling|
Application DetailsProduct Details Insulin ELISA Kit Target details Handling References for Insulin Kit (ABIN416266) Images back to top
Information on standard material:
|Sample Volume||50 μL|
|Assay Time||2.5 h|
|Plate||Pre-coated,Strips (12 x 8)|
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to INS has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled INS and unlabeled INS (Standards or samples) with the pre-coated antibody specific to INS. After incubation the unbound conjugate is washed off.
Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of INS in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of INS in the sample.
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between INS concentration in the sample and the assay signal intensity. Average the duplicate readings for each standard, control, and samples. Create a standard curve on log-log or semi-log graph paper, with the log of INS concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.
Intra-assay Precision (precision within an assay): 3 samples with low, middle and high level INS were tested 20 times on one plate, respectively.
Inter-assay Precision (precision between assays): 3 samples with low, middle and high level INS were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/mean X 100
|Restrictions||For Research Use only|
HandlingProduct Details Insulin ELISA Kit Target details Application Details References for Insulin Kit (ABIN416266) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
|Handling Advice||To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.|
|Storage||4 °C/-20 °C|
|Expiry Date||6 months|
References for Insulin Kit (ABIN416266)Product Details Insulin ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Wang, Zhou, Quach, Lu, Gao, Xu, Zhu: "Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model." in: Obesity surgery, Vol. 26, Issue 4, pp. 797-804, 2016
Zhou, Guo, Huang, Zhu, Fan, Wang, Wang, Zhu, Xu, Wu, Lu, Wang: "The dynamic three-dimensional culture of islet-like clusters in decellularized liver scaffolds." in: Cell and tissue research, Vol. 365, Issue 1, pp. 157-71, 2016
Olatunji, Omolekulo, Usman, Kim: "Improvement of oral contraceptive-induced glucose dysregulation and dyslipidemia by valproic acid is independent of circulating corticosterone." in: Archives of physiology and biochemistry, Vol. 122, Issue 3, pp. 123-9, 2016
Fang, Yu, He, Guo, Huang, Kong, Shi, Zhu, Bo, Zhang: "Central injection of GALR1 agonist M617 attenuates diabetic rat skeletal muscle insulin resistance through the Akt/AS160/GLUT4 pathway." in: Mechanisms of ageing and development, 2016
Ali, El-Sayyad, Moftah, Chilibeck: "Structural and functional abnormalities of hepatic tissues in male Wistar rats fed hyperwhey and super amino anabolic protein." in: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 32, Issue 7-8, pp. 840-8, 2016
Lavet, Martin, Linossier, Vanden Bossche, Laroche, Thomas, Gerbaix, Ammann, Fraissenon, Lafage-Proust, Courteix, Vico: "Fat and Sucrose Intake Induces Obesity-Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 31, Issue 1, pp. 98-115, 2016
Olatunji, Usman, Seok, Kim: "Activation of cardiac renin-angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder." in: Archives of physiology and biochemistry, pp. 1-8, 2016
Abu, Samat, Kamarapani, Nor Hussein, Wan Ismail, Hassan: "Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 985042, 2015
Nascimento da Silva, Azevedo de Jesuz, De Salvo Castro, Soares da Costa, Teles Boaventura, Blondet de Azeredo: "Effect of the “protein diet” and bone tissue." in: Nutrición hospitalaria, Vol. 29, Issue 1, pp. 140-5, 2014
Yang, Luo, Zhou, Lv, Liu, Zhang, Gao, Chen, Xia, Luo, Cheng, Li: "Rosiglitazone inhibits expression and secretion of PEDF in adipose tissue and liver of male SD rats via a PPAR-? independent mechanism." in: Endocrinology, Vol. 155, Issue 3, pp. 941-50, 2014
Mohammadi, Gholamhoseinian, Fallah: "Zataria multiflora increases insulin sensitivity and PPAR? gene expression in high fructose fed insulin resistant rats." in: Iranian journal of basic medical sciences, Vol. 17, Issue 4, pp. 263-70, 2014
Tinkov, Popova, Polyakova, Nikonorov: "Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 27, Issue 2, pp. 293-303, 2014
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