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Rat (Rattus) Insulin ELISA Kit

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Antigen
Insulin (INS) ELISA Kits
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • insulin-like
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • Ins2
  • LOC100060077
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
62
28
26
16
15
12
11
11
11
9
7
6
4
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
5-300 μIU/mL
Minimum Detection Limit
5 IU/mL
Application
ELISA
Options
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Catalog No. ABIN1979556
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
11.926027 ABIN416266 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 123.5-10000 pg/mL 123.5 pg/mL Log in to see 12
11.926027 ABIN366470 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 15.6-1000 nlU/mL 15.6 nlU/mL Log in to see 11
11.926027 ABIN366486 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 1.56-100 pg/mL 1.56 pg/mL Log in to see 1
11.926027 ABIN1116659 Sandwich ELISA Serum, Plasma, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
11.926027 ABIN2703271 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4-300 μIU/mL 4 IU/mL Log in to see
11.926027 ABIN2703269 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.5-300 μIU/mL 4.5 IU/mL Log in to see
1 ABIN1028897 Competition ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 111.11-9000 pg/mL 111.11 pg/mL Log in to see
1 ABIN771959 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN1873118 Competition ELISA Plasma, Cell Lysate, Cell Culture Supernatant, Serum, Tissue Homogenate, Biological Fluids 78.12-20000 pg/mL 78.12 pg/mL Log in to see
1 ABIN1057579 Sandwich ELISA Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma 5.0-100 pM/L 5.0 pM/L Log in to see
1 ABIN1813713 Sandwich ELISA Body Fluids, Tissue Lysate, Cell Culture Supernatant, Serum Log in to see 3
1 ABIN772198 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
1 ABIN1774778 Serum, Plasma 0-100 μIU/mL 0 μIU/mL Log in to see 2
1 ABIN4993536 Sandwich ELISA Plasma, Serum, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN2684755 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 0-140 μIU/mL Log in to see
1 ABIN1846977 Serum, Plasma, Biological Fluids 0-140 μIU/mL 0 μIU/mL Log in to see
1 ABIN2535031 Competition ELISA Serum, Plasma, Biological Fluids 111.11-9000 pg/mL 111.11 pg/mL Log in to see
1 ABIN2948503 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN2950654 0.313-20 ng/mL 0.313 ng/mL Log in to see
1 ABIN4970136 Sandwich ELISA Plasma, Serum, Biological Fluids 0.2-6.9 ng/mL 0.2 ng/mL Log in to see

Top referenced Insulin ELISA Kit for ELISA

Similar Insulin ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 28 ELISA Kits

General

Antigen Insulin (INS) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(62), (28), (26), (16), (15), (12), (11), (11), (11), (9), (7), (6), (4), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 5-300 μIU/mL
Minimum Detection Limit 5 IU/mL
Application ELISA
Pubmed 5 references available
Supplier Log in to see

Product Details Insulin ELISA Kit

Target details Application Details Handling References for Insulin Kit (ABIN1979556) Images
Purpose Rat Insulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Serum, Plasma, Cell Culture Supernatant
Analytical Method Quantitative
Specificity This ELISA antibody pair detects rat, mouse, human, porcine and bovine Insulin and Proinsulin.
Sensitivity 5 μIU/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details Insulin ELISA Kit Application Details Handling References for Insulin Kit (ABIN1979556) Images back to top
Antigen
Alternative Name Insulin (INS ELISA Kit Abstract)
Gene ID 24506
UniProt P01323
Research Area Neurology, Diabetes, Hormones
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour

Application Details

Product Details Insulin ELISA Kit Target details Handling References for Insulin Kit (ABIN1979556) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. 2. Sample dilution: If your samples need to be diluted, Assay Diluent C (Item L) should be used for dilution of serum/plasma/culture supernatants/urine. Suggested dilution for normal serum/plasma: 2 fold*. The Rat Insulin ELISA Kit Protocol 3 * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator. 3. Assay Diluent B (Item E) should be diluted 5-fold with deionized or distilled water before use. 4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL Assay Diluent C (Item L) into Item C vial to prepare a 1,400 µIU/ml standard solution. Dissolve the powder thoroughly by a gentle mix. Add 150 µL Insulin standard (1,400 µIU/ml) from the vial of Item C, into a tube with 550 µL Assay Diluent C to prepare a 300 µIU/ml standard solution. Pipette 250myl Assay Diluent C into each tube. Use the 300 µIU/ml standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Assay Diluent C serves as the zero standard (0 µIU/ml). 250 µL 250 µL 250 µL 250 µL 250myl 180 µL Standard + 450 µL 250 µL 300 150 75 37.5 18.75 9.38 4.69 0 µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml µIU/ml 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 mL of Wash Buffer Concentrate into deionized or distilled water to yield 400 mL of 1x Wash Buffer. The Rat Insulin ELISA Kit Protocol 4 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B (Item E) into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 400-fold with 1x Assay Diluent B (Item E). For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 30 µL of HRP-Streptavidin concentrate into a tube with 12 mL 1x Assay Diluent B to prepare a 400-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking. 3. Discard remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Assay Precision Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only

Handling

Product Details Insulin ELISA Kit Target details Application Details References for Insulin Kit (ABIN1979556) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for Insulin Kit (ABIN1979556)

Product Details Insulin ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Ding, Tong, Feng, Chen, Wang, Wang, Li, Zhu, Zhou: "Adipose afferent reflex response to insulin is mediated by melanocortin 4 type receptors in the paraventricular nucleus in insulin resistance rats." in: Acta physiologica (Oxford, England), Vol. 214, Issue 4, pp. 450-66, 2015

Shihabudeen, Roy, James, Thirumurugan: "Chenodeoxycholic acid, an endogenous FXR ligand alters adipokines and reverses insulin resistance." in: Molecular and cellular endocrinology, Vol. 414, pp. 19-28, 2015

Lee, Seo, Ryu, Kwon: "The effect of swimming exercise and powdered-Salicornia herbacea L. ingestion on glucose metabolism in STZ-induced diabetic rats." in: Journal of exercise nutrition & biochemistry, Vol. 19, Issue 3, pp. 235-45, 2015

Al-Gayyar, Alyoussef, Hamdan, Abbas, Darweish, El-Hawwary: "Cod liver oil ameliorates sodium nitrite-induced insulin resistance and degradation of rat hepatic glycogen through inhibition of cAMP/PKA pathway." in: Life sciences, Vol. 120, pp. 13-21, 2014

Defranoux, Jutisz, Jerome, Job: "[Study of the effects of human growth hormone in a patient with pituitary dwarfism]." in: Annales d'endocrinologie, Vol. 26, Issue 4, pp. 501-12, 1970

Images

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Supplier Images
ELISA image for Insulin ELISA Kit (INS) (ABIN1979556) Insulin (INS) ELISA Kit