We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Mouse (Murine)||Human|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||24 Antibodies||77 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies||8 Antibodies|
|Flow Cytometry (FACS)||25 Antibodies||70 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||25 Antibodies|
|Immunofluorescence (IF)||28 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|SDS-PAGE (SDS)||1 Antibodies|
|Western Blotting (WB)||91 Antibodies|
|Antigen||Myeloid Differentiation Primary Response Gene (88) (MYD88) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This MYD88 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MYD88 AntibodyTarget Details MYD88 Application Details Handling References for anti-MYD88 Antibody (ABIN401364) Images
|Specificity||This antibody reacts to MYD88.|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to a region near the carboxy terminus of human MyD88 protein|
Target Details MYD88Product Details anti-MYD88 Antibody Application Details Handling References for anti-MYD88 Antibody (ABIN401364) Images back to top
|Alternative Name||MYD88 (MYD88 Antibody Abstract)|
|Background||MyD88 (Myeloid differentiation primary response protein). The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kappaΒ activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), both members of the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to the bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.Synonyms: Myeloid differentiation primary response protein MyD88|
|Pathways||NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MYD88 Antibody Target Details MYD88 Handling References for anti-MYD88 Antibody (ABIN401364) Images back to top
ELISA: 1/5000 - 1/20000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MYD88 Antibody Target Details MYD88 Application Details References for anti-MYD88 Antibody (ABIN401364) Images back to top
|Concentration||0.5 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.02 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-MYD88 Antibody (ABIN401364)Product Details anti-MYD88 Antibody Target Details MYD88 Application Details Handling Images back to top
Kawai, Adachi, Ogawa, Takeda, Akira: "Unresponsiveness of MyD88-deficient mice to endotoxin." in: Immunity, Vol. 11, Issue 1, pp. 115-22, 1999
Adachi, Kawai, Takeda, Matsumoto, Tsutsui, Sakagami, Nakanishi, Akira: "Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function." in: Immunity, Vol. 9, Issue 1, pp. 143-50, 1998
Medzhitov, Preston-Hurlburt, Kopp, Stadlen, Chen, Ghosh, Janeway: "MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways." in: Molecular cell, Vol. 2, Issue 2, pp. 253-8, 1998
Bonnert, Garka, Parnet, Sonoda, Testa, Sims: "The cloning and characterization of human MyD88: a member of an IL-1 receptor related family." in: FEBS letters, Vol. 402, Issue 1, pp. 81-4, 1997
Muzio, Ni, Feng, Dixit: "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1612-5, 1997
ImagesProduct Details anti-MYD88 Antibody Target Details MYD88 Application Details Handling References for anti-MYD88 Antibody (ABIN401364) back to top