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anti-Human NFKBIA antibody for Immunofluorescence

Recommended NFKBIA Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
  • AI462015
  • ECI-6/IKBA
  • IKBA
  • MAD-3
  • NFKBI
  • Nfkbi
  • RL/IF-1
Epitope
pSer32, pSer36
68
52
49
37
23
22
22
22
18
15
14
13
13
13
7
5
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
478
216
178
37
31
23
9
6
2
Host
Rabbit
330
153
5
1
Clonality
Polyclonal
Conjugate
This NFKBIA antibody is un-conjugated
19
14
13
13
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
367
187
145
86
86
78
73
35
34
19
6
4
4
3
2
2
1
1
1
1
1
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Catalog No. ABIN1531880
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.24714619 ABIN968112 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5
0.24714619 ABIN2856763 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.24714619 ABIN2707586 IC IF IHC WB Rabbit N-Term, pSer32, pSer36 Log in to see Polyclonal
0.24714619 ABIN2707587 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
0.24714619 ABIN2707588 IC IF IP IHC WB Rabbit N-Term Log in to see Polyclonal
0.24714619 ABIN1999491 IF ELISA Mouse IgG2b AA 2-317 Log in to see 2
0.24714619 ABIN1999494 FACS IF FITC Mouse IgG2b AA 2-317 Log in to see 2
0.24714619 ABIN2448340 ICC IF IHC (fp) WB Rabbit IgG Log in to see Polyclonal
0.24714619 ABIN2424901 IF IHC WB Rabbit IgG pSer32, pSer36 Log in to see Polyclonal
0.24714619 ABIN1326940 IF ELISA WB Mouse IgG2a kappa AA 1-317, full length Log in to see 3H4
0.24714619 ABIN2404044 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.24714619 ABIN4324166 ICC IF IHC IHC (p) WB Rabbit pSer32, pSer36 Log in to see Polyclonal
0.24714619 ABIN4324216 IF IHC WB Mouse IgG1 Log in to see 2F5
0.24714619 ABIN3032048 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 12-41 Log in to see Polyclonal
0.24714619 ABIN4324170 ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.0010816128 ABIN652417 FACS IF IHC (p) WB Rabbit Ig AA 12-41, Ser32, Ser36 Log in to see Polyclonal 2
0.0010816128 ABIN252471 FACS ICC IF IHC IHC (p) IP SimWes WB Mouse IgG1 kappa AA 32-291 Log in to see 6A920 32
0.0010816128 ABIN196874 IF IHC (p) WB Rabbit pSer32, pSer36 Log in to see Polyclonal 5
0.0010816128 ABIN197304 IF IHC (p) WB Rabbit Ser32, Ser36 Log in to see Polyclonal 5
0.0010816128 ABIN968113 IF IP IHC WB Mouse IgG1 AA 145-302 Log in to see 25-IkBa-MAD 5

Top referenced anti-NFKBIA antibody for Immunofluorescence

Similar anti-NFKBIA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 45 Antibodies 46 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 19 Antibodies 21 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 22 Antibodies 25 Antibodies
Immunofluorescence (IF) 44 Antibodies 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 134 Antibodies

General

Antigen Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies
Epitope pSer32, pSer36
(68), (52), (49), (37), (23), (22), (22), (22), (18), (15), (14), (13), (13), (13), (7), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(478), (216), (178), (37), (31), (23), (9), (6), (2)
Host Rabbit
(330), (153), (5), (1)
Clonality
Polyclonal
Conjugate This NFKBIA antibody is un-conjugated
(19), (14), (13), (13), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(367), (187), (145), (86), (86), (78), (73), (35), (34), (19), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFKBIA Antibody

Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1531880) Images
Specificity IkappaB-alpha (Phospho-Ser32/Ser36) Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at Ser32/Ser36.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Ser32/Ser36.
Isotype IgG

Target Details NFKBIA

Product Details anti-NFKBIA Antibody Application Details Handling References for anti-NFKBIA Antibody (ABIN1531880) Images back to top
Antigen
Alternative Name IkappaB-alpha (NFKBIA Antibody Abstract)
Background Molecular weight of target protein: 35 kDa
Synonyms: I-kappa-B-alpha, IkappaBalpha, IKBA, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappaB inhibitor alpha, NFKBI, NFKBIA, RL/IF-1
Molecular Weight 35 kDa
Gene ID 4792
OMIM 164008
UniProt P25963
Research Area Transcription Factors
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFKBIA Antibody Target Details NFKBIA Handling References for anti-NFKBIA Antibody (ABIN1531880) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.81328 (NCBI Gene Symbol: NFKBIA)

Restrictions For Research Use only

Handling

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details References for anti-NFKBIA Antibody (ABIN1531880) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-NFKBIA Antibody (ABIN1531880)

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling Images back to top
Background publications

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991

Images

Product Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling References for anti-NFKBIA Antibody (ABIN1531880) back to top
Supplier Images
Western Blotting (WB) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pSer32) (ABIN1531880) Western blot analysis of extracts from COS7 cells, using IkappaB-alpha (Phospho-Ser32...
Immunohistochemistry (IHC) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pSer32) (ABIN1531880) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Ikap...
Immunofluorescence (IF) image for anti-NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pSer32) (ABIN1531880) Immunofluorescence analysis of LOVO cells, using IkappaB-alpha (Phospho-Ser32/Ser36) ...