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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies||2 Antibodies|
|ELISA||58 Antibodies||60 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||21 Antibodies||22 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||18 Antibodies|
|Immunofluorescence (IF)||50 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||27 Antibodies|
|Immunohistochemistry (IHC)||97 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||59 Antibodies|
|Immunoprecipitation (IP)||27 Antibodies|
|Simple Western (SimWes)||2 Antibodies|
|Western Blotting (WB)||165 Antibodies|
|Antigen||Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This NFKBIA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-NFKBIA AntibodyTarget Details NFKBIA Application Details Handling Images
|Specificity||This antibody detects endogenous levels of IkappaB-alpha protein. (region surrounding Leu26)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details NFKBIAProduct Details anti-NFKBIA Antibody Application Details Handling Images back to top
|Alternative Name||NFKBIA / IKBA (NFKBIA Antibody Abstract)|
|Background||The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkappaB-alpha and specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation Serines 32 and 36. IKKalpha appears to be critical for NFkappaB activation in response to proinflammatory cytokines. Phosphorylation of IkappaB by IKKalpha is stimulated by the NFkappaB inducing kinase (NIK), which itself is a central regulator for NFkappaB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI|
|Molecular Weight||approx. 36 kDa|
|Pathways||NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-NFKBIA Antibody Target Details NFKBIA Handling Images back to top
ELISA: 1: 10000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-NFKBIA Antibody Target Details NFKBIA Application Details Handling back to top