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anti-Human PRKACA antibody for Western Blotting

Recommended PRKACA Antibody (supplied by: Log in to see )

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • PKCD
  • Pkaca
  • PKACA
  • PKA
  • Calpha
  • Cs-PKA
  • PKCA1
  • PRKAR1
  • protein kinase, cAMP dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, regulatory, type I, beta
  • Prkaca
  • PRKACA
  • PRKAR1B
Epitope
N-Term
12
12
7
7
6
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
91
27
22
2
1
1
Host
Rabbit
58
29
4
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
76
25
13
13
9
8
5
5
2
2
1
1
1
Supplier
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Catalog No. ABIN359056
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0741873 ABIN453298 EIA WB Rabbit N-Term Log in to see Polyclonal 1
3.0741873 ABIN238374 WB Mouse IgG1 Log in to see 6D2-1 5
3.0741873 ABIN2787416 WB Rabbit N-Term Log in to see Polyclonal
3.0741873 ABIN2787415 WB Rabbit N-Term Log in to see Polyclonal
3.0741873 ABIN390798 FACS IHC (p) WB Rabbit Ig AA 67-94, N-Term Log in to see Polyclonal
3.0741873 ABIN408069 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
3.0741873 ABIN2855997 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.0741873 ABIN2856513 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
3.0741873 ABIN4904855 WB Rabbit IgG Log in to see Polyclonal
3.0741873 ABIN407415 WB Mouse IgG1 kappa Log in to see 3B2
3.0741873 ABIN4345785 ELISA WB Mouse IgG1 Log in to see 7H3A4
3.0741873 ABIN633167 WB Rabbit N-Term Log in to see Polyclonal
3.0741873 ABIN4902742 ELISA WB Goat Internal Region Log in to see Polyclonal
3.0741873 ABIN4902743 ELISA WB Goat Internal Region Log in to see Polyclonal
3.0741873 ABIN4345784 IP WB Rabbit Log in to see Polyclonal
3.0741873 ABIN519174 WB Rabbit AA 1-351, full length Log in to see Polyclonal
3.0741873 ABIN929010 WB Rabbit N-Term Log in to see Polyclonal
3.0741873 ABIN5078848 ICC IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN127012 IP WB Rabbit Catalytic Subunit Log in to see Polyclonal
1 ABIN562366 ELISA WB Mouse AA 1-120, partial Log in to see Polyclonal 1

Top referenced anti-PRKACA antibody for Western Blotting

  • Human Polyclonal PRKACA Primary Antibody for ICC, IF - ABIN408069 (1 Publications): Enns, Morton, Mangalindan, McKnight, Schwartz, Kaeberlein, Kennedy, Rabinovitch, Ladiges: Attenuation of age-related metabolic dysfunction in mice with a targeted disruption of the Cbeta subunit of protein kinase A. in The journals of gerontology. Series A, Biological sciences and medical sciences 2009 (PubMed)

  • Human Polyclonal PRKACA Primary Antibody for ELISA, WB - ABIN562366 (1 Publications): Adachi, Kano, Saido, Murata: Visual screening and analysis for kinase-regulated membrane trafficking pathways that are involved in extensive beta-amyloid secretion. in Genes to cells : devoted to molecular & cellular mechanisms 2009 (PubMed)

Similar anti-PRKACA Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 3 Antibodies 25 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 5 Antibodies
Immunofluorescence (IF) 6 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 16 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Epitope N-Term
(12), (12), (7), (7), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(91), (27), (22), (2), (1), (1)
Host Rabbit
(58), (29), (4), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(76), (25), (13), (13), (9), (8), (5), (5), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling References for anti-PRKACA Antibody (ABIN359056) Images
Specificity This antibody reacts to PKA C-alpha/PKA C-beta.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PKA/C.
Isotype Ig

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling References for anti-PRKACA Antibody (ABIN359056) Images back to top
Antigen
Alternative Name PRKACA (PRKACA Antibody Abstract)
Background CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by the PRKACA gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase.Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha
Gene ID 5566, 9606
UniProt P17612
Research Area Cancer, Kinases/Phosphatases
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling References for anti-PRKACA Antibody (ABIN359056) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details References for anti-PRKACA Antibody (ABIN359056) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PRKACA Antibody (ABIN359056)

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling Images back to top
Background publications

Tateyama, Rivolta, Clancy, Kass: "Modulation of cardiac sodium channel gating by protein kinase A can be altered by disease-linked mutation." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46718-26, 2003

Cartier, Hemonnot, Gay, Bardy, Sanchiz, Devaux, Briant: "Active cAMP-dependent protein kinase incorporated within highly purified HIV-1 particles is required for viral infectivity and interacts with viral capsid protein." in: The Journal of biological chemistry, Vol. 278, Issue 37, pp. 35211-9, 2003

Libersan, Rousseau, Merhi: "Differential regulation of P-selectin expression by protein kinase A and protein kinase G in thrombin-stimulated human platelets." in: Thrombosis and haemostasis, Vol. 89, Issue 2, pp. 310-7, 2003

Yasuda, Barth, Stellwagen, Malenka: "A developmental switch in the signaling cascades for LTP induction." in: Nature neuroscience, Vol. 6, Issue 1, pp. 15-6, 2002

Kaneider, Egger, Wiedermann, Ritter, Wöll, Wiedermann: "Involvement of cyclic adenosine monophosphate-dependent protein kinase A and pertussis toxin-sensitive G proteins in the migratory response of human CD14+ mononuclear cells to katacalcin." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 17, Issue 10, pp. 1872-82, 2002

Images

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling References for anti-PRKACA Antibody (ABIN359056) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PRKACA antibody (Protein Kinase A, alpha) (N-Term) (ABIN359056) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-PRKACA antibody (Protein Kinase A, alpha) (N-Term) (ABIN359056) The anti-PKA/C alpha/beta Pab is used in Western blot to detect PKA/C alpha in Jurkat...